|Application ||WB, IHC-P|
|Calculated MW||56528 Da|
|Purification||Antisera to human VISA were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.|
|Immunogen||A peptide corresponding to a 17 amino acid sequence from near the centre of Human VISA.|
|Shelf Life||18 months from the date of despatch.|
|Other Names||Mitochondrial antiviral-signaling protein, MAVS, CARD adapter inducing interferon beta, Cardif, Interferon beta promoter stimulator protein 1, IPS-1, Putative NF-kappa-B-activating protein 031N, Virus-induced-signaling adapter, VISA, MAVS, IPS1, KIAA1271, VISA|
|Target/Specificity||Rabbit anti-VISA antibody recognizes the human Mitochondrial antiviral-signaling protein (MAVS), also known as Virus-induced-signalling-adaptor (VISA), and KIAA1271, VISA is a 540 amino acid outer mitochondrial membrane protein containing a single CARD domain. VISA is an important protein required for innate immune defence against viruses (Huanget al.2014).VISA acts downstream of DDX58 and IFIH1/MDA5, which detect intracellular dsRNA produced during viral replication, to coordinate pathways leading to the activation of NF-kappa-B, IRF3 and IRF7, and to the subsequent induction of antiviral cytokines such as IFN-beta and RANTES (CCL5). VISA is believed to activate the same pathways following detection of extracellular dsRNA by TLR3 and it has been hypothosized that VISA is involved in the regulation of apoptosis following viral infection (Leiet al.2009).VISA is a ubiquitously expressed protein present in a range of cell types including T-cells, monocytes and epithelial cells, with highest levels in tissues of the heart, skeletal muscle, liver, placenta and in peripheral blood leukocytes. Multiple isoforms of VISA generated by alternative splicing have been identified. Rabbit anti-VISA antibody is reactive with full length VISA isoform 1. It does not react with isoforms 2,3,5 or 6, reactivity with isoform 4 is unknown.|
|Preservative & Stabilisers||0.02% Sodium Azide (NaN3)|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-VISA Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Seth, R.B., et al. (2006) Antiviral innate immunity pathways. Cell Res. 16:141-72. Xu LG, et al. (2005) VISA is an adapter protein required for virus-triggered IFN-beta signaling. Mol. Cell 19:727-40. 3. Meylan E, et al. (2005) Cardif is an adaptor protein in the RIG-I antiviral pathway and is targeted by hepatitis C virus. Nature 437(7062):1167-72.
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