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Anti-BAK (N-Terminal) Antibody

Rabbit Anti-Human Polyclonal Antibody

  • ICC - Anti-BAK (N-Terminal) Antibody  ABD10035
    Immunocytochemical staining of L1210 cells with Rabbit anti Human Bak
  • WB - Anti-BAK (N-Terminal) Antibody  ABD10035
    Western blot analysis of Whole cell lysates from L1210 mouse lymphocytic leukemia cells probed with Rabbit anti Human Bak at 1(A) and 2(B) ug/ml
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O08734
Other Accession Q16611
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 23295 Da
Additional Information
Other Species M
Purification Antiserum to BAK (NT) was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by ion exchange chromatography.
Immunogen A peptide corresponding to 13 amino acids near the amino terminus of human BAK.
Shelf Life 18 months from the date of despatch.
Gene ID 12018
Other Names Bcl-2 homologous antagonist/killer, Apoptosis regulator BAK, Bak1, Bak
Target/Specificity Rabbit anti-BAK (N-Terminal) antibody recognizes the Bcl-2 homologous antagonist/killer (Bak), an oligomeric protein localised to the outer mitochondrial membrane and the endoplasmic reticulum of healthy cells. Bak is a pro-apoptotic Bcl-2 family member similar to Bax with which it is thought to share significant homology. Mcl-1 and Bcl-xL interact with Bak and prevent the activation of Bak in healthy cells. Upon cytotoxic signalling, Mcl-1- and Bcl-xL-binding proteins (such as Noxa and Bad) displace Bak and apoptosis is induced. When activated, Bak (and Bax) permeabilises the outer membrane of the mitochondria or the ER to release proapoptogenic factors such as cytochrome c.
Preservative & Stabilisers 0.02% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-BAK (N-Terminal) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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Cat# ABD10035
(40 western blots)
Availability: Inquire
Bulk Size
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