|Application ||WB, IHC-F|
|Calculated MW||23295 Da|
|Purification||Antiserum to BAK (NT) was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by ion exchange chromatography.|
|Immunogen||A peptide corresponding to 13 amino acids near the amino terminus of human BAK.|
|Shelf Life||18 months from the date of despatch.|
|Other Names||Bcl-2 homologous antagonist/killer, Apoptosis regulator BAK, Bak1, Bak|
|Target/Specificity||Rabbit anti-BAK (N-Terminal) antibody recognizes the Bcl-2 homologous antagonist/killer (Bak), an oligomeric protein localised to the outer mitochondrial membrane and the endoplasmic reticulum of healthy cells. Bak is a pro-apoptotic Bcl-2 family member similar to Bax with which it is thought to share significant homology. Mcl-1 and Bcl-xL interact with Bak and prevent the activation of Bak in healthy cells. Upon cytotoxic signalling, Mcl-1- and Bcl-xL-binding proteins (such as Noxa and Bad) displace Bak and apoptosis is induced. When activated, Bak (and Bax) permeabilises the outer membrane of the mitochondria or the ER to release proapoptogenic factors such as cytochrome c.|
|Preservative & Stabilisers||0.02% Sodium Azide (NaN3)|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-BAK (N-Terminal) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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