|Application ||WB, IHC-F|
|Calculated MW||46159 Da|
|Purification||Antiserum to SIGIRR (CT) was raised by repeated immunisation of with highly purified antigen. Purified IgG was prepared from whole serum by immunoaffinity chromatography.|
|Immunogen||A synthetic peptide corresponding to 15 amino acids near the carboxy terminus of mouse SIGIRR.|
|Shelf Life||18 months from the date of despatch.|
|Other Names||Single Ig IL-1-related receptor, Single Ig IL-1R-related molecule, Single immunoglobulin domain-containing IL1R-related protein, Toll/interleukin-1 receptor 8, TIR8, Sigirr, Tir8|
|Target/Specificity||Rabbit anti-SIGIRR (C-terminal) antibody recognizes the Single Ig IL-1-related receptor, also known as SIGIRR, Single immunoglobulin domain-containing IL1R-related protein, Toll/interleukin-1 receptor 8 or TIR8. SIGIRR is a 409 amino acid ~ 45-90 kDa single pass type III transmembrane glycoprotein, Multiple species of SIGIRR seen in western blotting are achieved through differential glycosylation at four potential glycosylation sites in the SIGIRR ectodomain, mutation at these sites to eliminate glycosylation yields a single band of the predicted size (44 kDa) for unmodified SIGIRR (Zhaoet al.2015).SIGIRR is a member of the Toll-like receptor-interleukin 1 receptor superfamily, defined by an intracellular Toll-IL-1R (TIR) domain. SIGIRR acts as a negative regulator of the Toll-like and IL-1R receptor signalling pathways. It interacts with TLR4, TLR5, TLR9 and IL-1R through the extracellular Ig domain, or the intracellular TIR domain. The Ig domain interferes with the heterodimerization of IL-1R while the intracellular TIR domain inhibits both IL-1 and LPS signalling by attenuating the recruitment of receptor-proximal signalling components to TLR4. SIGIRR is expressed in kidney, colon, small intestine, lung, spleen and liver. It is not expressed in brain or muscle.Rabbit anti-SIGIRR antibody has been successfully used for the detection of SIGIRR in both murine and human samples by western blotting (Zhaoet al.2015)|
|Preservative & Stabilisers||0.02% Sodium Azide (NaN3)|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-SIGIRR (C-Terminal) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Zhao, J. et al. (2015) Human Colon Tumors Express a Dominant-negative Form of SIGIRR That Promotes Inflammation and Colitis-associated Colon Cancer in Mice.Gastroenterology. 24 Aug. [Epub ahead of print]
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