|Application ||WB, IHC-P|
|Calculated MW||34061 Da|
|Purification||Antiserum to human Caspase-7 (NT) was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.|
|Immunogen||A 16 amino acid peptide from human Caspase-7 amino-terminus.|
|Shelf Life||18 months from the date of despatch.|
|Other Names||Caspase-7, CASP-7, 188.8.131.52, Apoptotic protease Mch-3, Cysteine protease LICE2, Caspase-7 subunit p20, Caspase-7 subunit p11, Caspase-7, N-terminally processed, Casp7, Lice2, Mch3|
|Target/Specificity||Rabbit anti-Caspase-7 (N-Terminal) antibody recognizes an epitope within the N-Terminal region (NT) of Caspase-7, a 35kDa cysteine protease, otherwise known as ICE-like Apoptotic Protease 3 (ICE-LAP3).Caspase-7, a member of the ICE/Ced-3 subfamily, is an executioner caspase which undergoes proteolytic cleavage of its precursor to form active p12 and p20 subunits. Evidence that activation of Caspase-7 occurs during cell death induced by the cytokine death receptors Fas/APO-1 and the receptor of tumour necrosis factor (TNFR-1), coupled with the fact that granzyme-B activated Caspase-7 cleaves the nuclear enzyme poly (ADP-ribose) polymerase (PARP), suggests an important role for Caspase-7 in both cytokine-mediated and Granzyme-B mediated apoptosis pathways.|
|Preservative & Stabilisers||0.02% Sodium Azide (NaN3)|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Caspase-7 (N-Terminal) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Duan, H. et al. (1996) ICE-LAP3, a novel mammalian homologue of the Caenorhabditis elegans cell death protein Ced-3 is activated during Fas- and tumor necrosis factor-induced apoptosis.J. Biol Chem. 271: 1621 - 1625.2. Juan, T.S. et al. (1997) Identification and mapping of Casp7, a cysteine protease resembling CPP32 beta, interleukin-1 beta converting enzyme, and CED-3.Genomics. 40: 86-93.3. Cohen, G.M. (1997) Caspases: the executioners of apoptosis.J. Biochem. 326: 1 - 16.
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