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Anti-Human MAP1LC3A/B (N-Terminal) Antibody

Rabbit Anti-Human Polyclonal Antibody

  • WB - Anti-Human MAP1LC3A/B (N-Terminal) Antibody  ABD10044
    Western blotting analysis of LC3 expression on Atg5 knockout and wild type cells using Rabbit anti Human MAP1LC3A/B . Image courtesy of Dr. R. Kapito, Stanford University
  • WB - Anti-Human MAP1LC3A/B (N-Terminal) Antibody  ABD10044
    Published customer image:Rabbit anti Human MAP1LC3A/B antibody used for the detection of LC3-I and LC3-II in human mammary epitheial whole cell lysates by western blotting.Image caption:Cytoplasmic PELP1 and ERR? protect cells from Tam-induced autophagy. A, Cells in G1/G0 from cell cycle analysis of propidium iodide stained HMEC-LXSN and HMEC-Cyto cells treated with vehicle control or 1.0 µM Tam for 24 hours. A student’s T-Test was performed to determine statistical significance for changes in G1/G0 cell populations in response to Tam. P-values are given for the indicated comparisons. B, MTT assay of HMEC-hTERT cells treated with 0.5 or 1.0 µM Tam and ethanol control or 25 µM Z-VAD-FMK. A student’s T-Test was performed to determine statistical significance between control and Z-VAD-FMK treated cells in the presence of Tam. “ns” indicates no statistically significant differences were observed. C, Annexin V/7-AAD staining of HMEC-LXSN and HMEC-Cyto cells treated with 1.0 µM Tam for 24 hours. Student’s T-test was performed to determine statistical significance between Tam treated HMEC-LXSN and HMEC-cyto cells; p-values are shown for the indicated comparisons. D, Western blotting for LC3-I and LC3-II from HMEC-LXSN, HMEC-WT-1/WT-2, and HMEC-cyto lysates treated with ethanol control, 1 µM Tam, or 2 µM Tam for 48 hours. E, Western blot for LC3-II of whole cell lysates from HMECs expressing control or ERR? overexpression vector, treated with control or 0.5, 1, or 2 µM Tam for 24 hours. F, Western blot for LC3 from whole cell lysates of HMEC-LXSN and HMEC-cyto cells expressing either shControl or shERR?, treated with ethanol control or 1 µM Tam for 24 hours.From: Girard BJ, Regan Anderson TM, Welch SL, Nicely J, Seewaldt VL, et al. Cytoplasmic PELP1 and ERRgamma Protect Human Mammary Epithelial Cells from Tam-Induced Cell Death.PLoS ONE 10(3): e0121206.
  • WB - Anti-Human MAP1LC3A/B (N-Terminal) Antibody  ABD10044
    HeLa lysate probed with Rabbit anti Human MAP1LC3A/B (N-terminal) followed by Sheep anti Rabbit IgG:HRP
  • IF - Anti-Human MAP1LC3A/B (N-Terminal) Antibody  ABD10044
    HeLA cells were fixed with 3% PFA and permeabilized with 0.3% Triton X-100. Nuclei were stained with 1.5 µg/ml DAPI. Immunofluorescence was carried out using AHP2167, a Rabbit polyclonal anti-Human Microtubule associated protein 1LC3A/B (MAP1LC3A/B) at 1/25. STAR36D488GA, Sheep anti-Rabbit DyLight®488 was then applied at 1/50. Image was visualized on the Bio-Rad ZOE™ Fluorescent Cell Imager at 60x magnification.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9CQV6
Other Accession Q9GZQ8, Q9H492, Q91VR7
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 14617 Da
Additional Information
Other Species M
Purification Antiserum to human LC3A was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Immunogen Synthetic peptide sequence PSDRPFKQRRSFADC from the N-Terminal region of LC3A (NP_115903.1; NP_852610.1).
Shelf Life 18 months from date of despatch.
Gene ID 67443
Other Names Microtubule-associated proteins 1A/1B light chain 3B, Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1 light chain 3-like protein 2, MAP1A/MAP1B light chain 3 B, MAP1A/MAP1B LC3 B, Microtubule-associated protein 1 light chain 3 beta, Map1lc3b, Map1alc3, Map1lc3
Target/Specificity Rabbit anti-Human MAP1LC3A/B (N-Terminal) antibody specifically recognizes an epitope within the N-Terminal (NT) region of both MAP1LC3A (Microtubule-associated proteins 1A/1B light chain 3A/LC3A) and MAP1LC3B (Microtubule-associated proteins 1A/1B light chain 3B/LC3B), ubiquitin-like proteins and members of the MAP1LC3 family, which are widely used as reliable markers for the monitoring of autophagy.LC3-I is the cytosolic form of LC3, which is converted into the active, membrane-bound form LC3-II, during the autophagy process. Tracking the level of conversion of LC3-I to LC3-II provides an indicator of autophagic activity, and levels of LC3-II in particular, correlate with the extent of autophagosome formation, due to its association with the autophagosome membrane.Rabbit anti-Human MAP1LC3A/B (N-Terminal) antibody recognizes both the LC3-I and LC3-II forms of MAP1LC3A and MAP1LC3B.
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human MAP1LC3A/B (N-Terminal) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Iwata, A. et al. (2005) HDAC6 and microtubules are required for autophagic degradation of aggregated huntingtin.
J. Biol. Chem. 280: 40282-40292. 2. Riley, B.E. et al. (2010) Ubiquitin accumulation in autophagy-deficient mice is dependent on the Nrf2-mediated stress response pathway: a potential role for protein aggregation in autophagic substrate selection.
J. Cell. Biol. 191: 537-552. 3. Gjyshi, O. et al. (2015) Kaposi's Sarcoma-Associated Herpesvirus Induces Nrf2 Activation in Latently Infected Endothelial Cells through SQSTM1 Phosphorylation and Interaction with Polyubiquitinated Keap1.
J Virol. 89: 2268-86 4. Huang, L. et al. (2014) AKI after conditional and kidney-specific knockdown of stanniocalcin-1.
J Am Soc Nephrol. 25: 2303-15. 5. Girard, B.J. et al. (2015) Cytoplasmic PELP1 and ERRgamma Protect Human Mammary Epithelial Cells from Tam-Induced Cell Death.
PLoS One. 10 (3): e0121206.

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Cat# ABD10044
(40 western blots)
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