|Application ||WB, IHC-P, IP|
|Calculated MW||21184 Da|
|Purification||Antisera to human BAX was raised by repeated immunisation of rabbits with highly purified antigen.|
|Immunogen||Synthetic peptide, CPELALDPVPQDASTKKLSE, corresponding to amino acids 43-61 of human BAX.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Apoptosis regulator BAX, Bcl-2-like protein 4, Bcl2-L-4, BAX, BCL2L4|
|Target/Specificity||Rabbit anti-Human BAX antibody recognizes the human BAX protein, a proapoptotic member of the Bcl-2 family. BAX accelerates programmed cell death by binding to and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. It also induces the release of cytochrome C , activation of CASP3 and thereby apoptosis. Defects in BAX are found in some cell lines from hematopoietic malignancies as T-cell acute lymphoblastic leukemia, Burkitt lymphoma, and plasmacytoma.BAX migrates as a 21kD band in western blotting applications.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human BAX Antibody (Serum) is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Krajewski, S. et al. (1995) Reduced expression of proapoptotic gene BAX is associated with poor response rates to combination chemotherapy and shorter survival in women with metastatic breast adenocarcinoma.Cancer Res. 55: 4471-4478. 2. Condon, L.T. et al. (2002) Overexpression of Bcl-2 in squamous cell carcinoma of the larynx: a marker ofradioresistance.Int J Cancer. 100: 472-5. 3. Gonzalez-Campora, R. et al. (2007) BCL-2, TP53 and BAX protein expression in superficial urothelial bladder carcinoma.Cancer Lett. 250: 292-9. 4. Siu, W.P. et al. (2008) Bax-mediated mitochondrial outer membrane permeabilization (MOMP), distinct from the mitochondrial permeability transition, is a key mechanism in diclofenac-induced hepatocyte injury: Multiple protective roles of cyclosporin A.Toxicol Appl Pharmacol. 227: 451-61. 5. Leong, A.S. et al. (2008) Apoptosis is a major cause of so-called"caseous necrosis"in mycobacterial granulomas in HIV-infected patients.J Clin Pathol. 61: 366-72. 6. Wang, C.Y. et al. (2009) Glycogen synthase kinase-3 and Omi/HtrA2 induce annexin A2 cleavage followed by cell cycle inhibition and apoptosis.Mol Biol Cell. 20: 4153-61. 7. Feinmesser, M. et al. (2003) Differential expression of proliferation- and apoptosis-related markers in lentigo maligna and solar keratosis keratinocytes.Am J Dermatopathol. 25: 300-7.
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