|Application ||WB, IHC-P|
|Calculated MW||31608 Da|
|Purification||Antisera to human Caspase-3 were raised by repeated immunisation of rabbits with highly purified antigen.|
|Immunogen||Recombinant human caspase-3.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Caspase-3, CASP-3, 22.214.171.124, Apopain, Cysteine protease CPP32, CPP-32, Protein Yama, SREBP cleavage activity 1, SCA-1, Caspase-3 subunit p17, Caspase-3 subunit p12, CASP3, CPP32|
|Target/Specificity||Rabbit anti-Human Caspase-3 antibody recognizes the Caspase-3 cell death protease, also known as CPP32, YAMA and apopain. Caspase-3 is a ~32 kDa protein, activated during apoptosis by cleavage into 17 kDa and 11 kDa fragments. Rabbit anti-Human Caspase-3 antibody recognizes both intact Caspase-3 and the 17 kDa fragment, but not the 11 kDa fragment.In immunohistochemical studies Caspase-3 expression has been shown to be widespread, but not present in all cell types. Differences in the level of Caspase-3 have been reported in cells of short-lived nature (e.g. germinal centre B cells) and those that are long lived (e.g. mantle zone B cells).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human Caspase-3 Antibody (Serum) is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Krajewski, M. et al. (1997) Immunohistochemical analysis of in vivo patterns of expression of CPP32 (Caspase-3), a cell death protease.Cancer Res. 57: 1605-1613. 2. Gabdoulkhakova, A. et al. (2007) High rate of mutation reporter gene inactivation during human T cell proliferation.Immunogenetics. 59: 135-43. 3. Smith, K. et al. (2011) Mono- and tri-cationic porphyrin-monoclonal antibody conjugates: photodynamic activity and mechanism of action.Immunology. 132: 256-65. 4. Nix, R.N. et al. (2007) Hemophagocytic macrophages harbor Salmonella enterica during persistent infection.PLoS Pathog. 3: e193. 5. Sallmyr, A. et al. (2004) Expression of DNA-dependent protein kinase in human granulocytes.Cell Res. 14: 331-40. 6. Sohn, J.H. et al. (2000) Caspase-3/CPP32 immunoreactivity and its correlation with frequency of apoptotic bodies in human prostatic carcinomas and benign nodular hyperplasias.Histopathology. 37: 555-60. 7. Sallmyr, A. et al. (2002) An inducible Ku86-degrading serine protease in human cells.Biochim Biophys Acta. 1593: 57-68. 8. Gaymes, T.J. et al. (2006) Histone deacetylase inhibitors (HDI) cause DNA damage in leukemia cells: a mechanism for leukemia-specific HDI-dependent apoptosis?Mol Cancer Res. 4 (8): 563-73.
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