|Application ||WB, IHC-P|
|Calculated MW||141840 Da|
|Purification||Antisera to human Apaf-1 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.|
|Immunogen||Synthetic peptide corresponding to amino acids 12-28 of human Apaf1|
|Shelf Life||18 months from date of despatch.|
|Other Names||Apoptotic protease-activating factor 1, APAF-1, APAF1, KIAA0413|
|Target/Specificity||Apaf1 (Apoptosis protease activating factor 1) is the mammalian homologue of the CED-4 cell death protein in C. Elegans. Apaf1 binds to cytochrome C and caspase-9 leading to caspase-9 activation, resulting in the beginning of the proteolytic cascade characteristic of apoptosis. Apaf1 can also associate with caspase-4 and caspase-8. ABD10052 recognises a 130kD band in Western blots.|
|Preservative & Stabilisers||0.02% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-APAF1 (aa12-28) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Zou, H. et al. (1997) Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3.Cell 90: 405-413.2. Cecconi, F. et al. (1998) Apaf-1 (CED-4 homolog) regulates programmed cell death in mammalian development.Cell 94: 727-737.3. Hu, Y. et al. (1998) Bcl-XL interacts with Apaf-1 and inhibits Apaf-1 dependent caspase-9 activation.Proc. Natl. Acad. Sci. USA. 95: 4386-4391.
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