|Application ||WB, IHC-F, IF, EM|
|Purification||Antisera to rat TGN38 were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography.|
|Immunogen||Recombinant fusion protein corresponding to extracellular domain of TGN38.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Trans-Golgi network integral membrane protein TGN38, Ttgn1|
|Target/Specificity||Sheep anti-Rat TGN38 antibody recognizes rat TGN38, a 357 amino acid single pass trans membrane glycoprotein found primarily in the Trans-golgi network, and acts as an excellent marker for this cellular organelle (Humphreyet al.1993).TGN38 is likely to have a role in intracellular transport (McNamaraet al.2004) and plays a role in morphological maintenance (Girotti and Banting 1996). It is the homologue of human TGN46 and macaque TGN47 (Ponnambalamet al.1996).|
|Preservative & Stabilisers||0.09% Sodium Azide; 1% Bovine Serum Albumin;|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Rat TGN38 Antibody (Serum) is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Vo, Y.P. et al. (2004) Recycling of the dense-core vesicle membrane protein phogrin in Min6 beta-cells.Biochem Biophys Res Commun. 324: 1004-10. 2. Prabhu, Y. et al. (2014) Defective Transport of the Obesity Mutant PC1/3 N222D Contributes to Loss of Function.Endocrinology. 155: 2391-401. 3. Ni-Komatsu, L. et al. (2008) Identification of quinolines that inhibit melanogenesis by altering tyrosinase family trafficking. Mol Pharmacol. 74:1576-86. 4. Mathews, P.M. et al. (2002) Alzheimer's disease-related overexpression of the cation-dependent mannose 6-phosphate receptor increases Abeta secretion: role for altered lysosomal hydrolase distribution in beta-amyloidogenesis.J Biol Chem. 277: 5299-307. 5. Phillips, S.E. et al. (2006) Specific and nonspecific membrane-binding determinants cooperate in targeting phosphatidylinositol transfer protein beta-isoform to the mammalian trans-Golgi network.Mol Biol Cell. 17: 2498-512. 6. Waugh, M.G. et al. (2011) Detergent-free isolation and characterization of cholesterol-rich membrane domains from trans-Golgi network vesicles.J Lipid Res. 52: 582-9. 7. Farah,. C.A. et al. (2006) Tau interacts with Golgi membranes and mediates their association with microtubules.Cell Motil Cytoskeleton. 63: 710-24. 8. Hesse, D. et al. (2010) Altered GLUT4 trafficking in adipocytes in the absence of the GTPase Arfrp1.Biochem Biophys Res Commun. 394: 896-903. 9. Miranda-Saksena, M. et al. (2002) In rat dorsal root ganglion neurons, herpes simplex virus type 1 tegument forms in the cytoplasm of the cell body.J Virol. 76: 9934-51. 10. Procino, G. et al. (2006) Adipocytes support cAMP-dependent translocation of aquaporin-2 from intracellular sites distinct from the insulin-responsive GLUT4 storage compartment.Am J Physiol Renal Physiol. 290: F985-94. 11. Han, L. et al. (2008) A large form of secretogranin III functions as a sorting receptor for chromogranin A aggregates in PC12 cells.Mol Endocrinol. 22: 1935-49. 12. Probst, O.C. et al. (2006) The 46-kDa mannose 6-phosphate receptor does not depend on endosomal acidification for delivery of hydrolases to lysosomes.J Cell Sci. 2006 Dec 119: 4935-43. 13. Wasmeier, C. et al. (2006) Rab38 and Rab32 control post-Golgi trafficking of melanogenic enzymes.J Cell Biol. 175: 271-81. 14. Hou, J.C. et al. (2006) A specific dileucine motif is required for the GGA-dependent entry of newly synthesized insulin-responsive aminopeptidase into the insulin-responsive compartment.J Biol Chem. 281: 33457-66. 15. Fortin, M.E. et al. (2006) Modulation of GJA1 turnover and intercellular communication by proinflammatory cytokines in the anterior pituitary folliculostellate cell line TtT/GF.Biol Reprod. 74: 2-12. 16. Vasile, E. et al. (2006) IntraGolgi distribution of the Conserved Oligomeric Golgi (COG) complex.Exp Cell Res. 312: 3132-41. 17. Acquatella-Tran Van Ba, I. et al. (2011) Reg-1a is a new neuronal secreted factor that stimulates neurite outgrowth via the Exostosin tumor-like 3 (EXTL3) receptor.J Biol Chem. 287: 4726-39. 18. Watanabe, T. et al. (2012) A unique ball-shaped Golgi apparatus in the rat pituitary gonadotrope: its functional implications in relation to the arrangement of the microtubule network.J Histochem Cytochem. 60: 588-602.2. Luzio, J.P. et al. (1990) Identification, sequencing and expression of an integral membrane protein of the trans-Golgi network (TGN38).Biochem J. 270: 97-102.
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