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Anti-Human TGN46 Antibody (Serum)

Sheep Anti-Human Polyclonal Antibody

  • IF - Anti-Human TGN46 Antibody (Serum) ABD10057
    Published customer image:Sheep anti Human TGN46 antibody used for the localization of the trans-golgi network in M10 cells by immunofluorescenceImage caption:Cellular distribution of LAPTM5. Transfected M10 cells co-expressing EYFP-LAPTM5 and CD1e were fixed, permeabilized and co-stained with the anti-CD1e mAb 20.6, revealed using Cy3-conjugated antibodies (A) and antibodies specific for TGN46, revealed by Cy5-conjugated antibodies (B, left panel, represented using red pseudo-color), or stained with anti-EEA1, -CD63, -Lamp1 (B) or -HLA-DR Abs (C). Untreated cells (upper panel) were compared with cells treated for 4 hours with bafilomycin (lower panel) (A). D) M10 cells co-expressing CD1e and EYFP-LAPTM5 were fixed and processed for immunolabeling of cryosections with 10 nm anti-GFP and 15 nm anti-CD1e conjugated gold particles. EYFP-LAPTM5 and CD1e were detected in the endoplasmic reticulum (ER), in Golgi compartments (Golgi) and in multivesicular (MVs) and multilamellar endosomes (MLs). E, F) Characterization of two anti-LAPTM5 mAbs. M10 cells co-expressing CD1e and EYFP-LAPTM5 were fixed, permeabilized and stained with an anti-LAPTM5 mAb (2.16.1 or 47.15.6), which was revealed with Cy3-conjugated secondary Abs (E). M10 cells expressing CD1e were fixed, permeabilized and double-labeled with 2.16.1 and either anti-TGN46, anti-EEA1, anti-CD63 or anti-HLA-DR Abs (F). Scale bars, confocal micrographs, 10 µM; electron microscopy micrographs, 100 nM.From: Angénieux C, Waharte F, Gidon A, Signorino-Gelo F, Wurtz V, Hojeij R, et al. Lysosomal-Associated Transmembrane Protein 5 Is a Molecular Partner of CD1e.PLoS ONE 7: e42634.
  • IF - Anti-Human TGN46 Antibody (Serum) ABD10057
    Published customer image:Sheep anti Human TGN46 antibody used to localize the trans-golgi network in HeLa cells by immunofluorescence.Image caption:C11ORF24 is present on RAB6 positive transport carriers. (A) HeLa cells expressing GFP-C11ORF24 were imaged every second for 5 minutes by spinning disk microscopy. The red arrow indicates a small transport carrier whereas the green arrow point to a long tube connected to the Golgi apparatus. A magnified view of these structures is shown in line 2 and 3. (B) HeLa cells were fixed and co-stained with TGN46, C11ORF24 and GTP-RAB6. Colocalization is very strong between TGN46, C11ORF24 and GTP-RAB6. A line profile of the C11ORF24 , TGN46 and GTP-RAB6 fluorescence intensities from the lines in the magnified views are shown in column 7. (C) HeLa cells expressing GFP-C11ORF24 and mCherry-RAB6 were imaged every second for 5 minutes by spinning disk microscopy. The arrow indicates a transport carrier positive for GFP-C11ORF24 and for mCherry-RAB6 . A magnified view of this structure is shown lines 4-6. (D) Transport carriers were visualized using a kymograph that was made along the line drawn on the merge image. All the dynamic elements positive for GFP-C11ORF24 (left and merge green) were positive for mCherry-RAB6 (middle and merge red).From: Fraisier V, Kasri A, Miserey-Lenkei S, Sibarita J-B, Nair D, Mayeux A, et al. C11ORF24 Is a Novel Type I Membrane Protein That Cycles between the Golgi Apparatus and the Plasma Membrane in Rab6-Positive Vesicles.PLoS ONE 8: e82223.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O43493
Reactivity Human
Host Sheep
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 51113 Da
Additional Information
Other Species Primate
Purification Antisera to human TGN46 were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography.
Immunogen Recombinant human TGN46
Shelf Life 18 months from date of despatch.
Gene ID 10618
Other Names Trans-Golgi network integral membrane protein 2, TGN38 homolog, TGN46, TGN48, Trans-Golgi network protein TGN51, TGOLN2, TGN46, TGN51
Target/Specificity Sheep anti-Human TGN46 antibody recognizes Trans-Golgi network integral membrane protein 2 (TGOLN2), also known as TGN38 homolog, TGN46, TGN48 or Trans-Golgi network protein TGN51. TGN46 is a 437 amino acid glycoprotein localized to the Trans-Golgi network. TGN46 has been reported as being the best available marker for human trans-Golgi network.TGN46 is a heavily glycosylated protein of around 110-120 kDa. Multiple isoforms of TGN46 are generated by alternative splicing difffering in sequence at the C-terminal protion. Sheep anti-Human TGN46 antibody is expeced to recognize all identified isoforms.
Preservative & Stabilisers 0.02% Sodium Azide (NaN3); 1% Bovine Serum Albumin; 25% Glycerol
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human TGN46 Antibody (Serum) is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)

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1. Prescott, A.R. et al. (1997) Distinct compartmentalization of TGN46 and beta 1, 4-galactosyltransferase in HeLa cells.
Eur. J. Cell Biol. 72: 238-246. 3. Vuillier, F. et al. (2005) Lower levels of surface B-cell-receptor expression in chronic lymphocytic leukemia are associated with glycosylation and folding defects of the mu and CD79a chains.
Blood. 105: 2933-2940. 4. Edwards, T.L. et al. (2009) Endogenous spartan (SPG20) is recruited to endosomes and lipid droplets and interacts with the ubiquitin E3 ligases AIP4 and AIP5.
Biochem. J. 423: 31-39. 5. Roberts, R.C. et al. (2010) Mistargeting of SH3TC2 away from the recycling endosome causes Charcot-Marie-Tooth disease type 4C.
Hum Mol Genet.19: 1009-18. 6. Hauser, H. et al. (2010) HIV-1 Vpu and HIV-2 Env counteract BST-2/tetherin by sequestration in a perinuclear compartment.
Retrovirology. 7: 51. 7. Oliver, S.L. et al. (2011) Mutagenesis of varicella-zoster virus glycoprotein I (gI) identifies a cysteine residue critical for gE/gI heterodimer formation, gI structure, and virulence in skin cells.
J Virol. 85 (9): 4095-110. 8. Berarducci, B. et al. (2006) Essential functions of the unique N-terminal region of the varicella-zoster virus glycoprotein E ectodomain in viral replication and in the pathogenesis of skin infection.
J Virol. 80: 9481-96. 9. Sadaoka, T. et al. (2010) Characterization of the varicella-zoster virus ORF50 gene, which encodes glycoprotein M.
J Virol. 84: 3488-502. 10. Vleck, S.E. et al. (2010) Anti-glycoprotein H antibody impairs the pathogenicity of varicella-zoster virus in skin xenografts in the SCID mouse model.
J Virol. 84: 141-52. 11. Esk, C. et al. (2010) The clathrin heavy chain isoform CHC22 functions in a novel endosomal sorting step.
J Cell Biol. 188: 131-44. 12. Cheng, S.B. et al. (2011) Down-modulation of the G-protein-coupled Estrogen Receptor, GPER, from the Cell Surface Occurs via a trans-Golgi-Proteasome Pathway.
J Biol Chem. 286: 22441-55. 13. Fairn, G.D. et al. (2011) High-resolution mapping reveals topologically distinct cellular pools of phosphatidylserine.
J Cell Biol. 194 (2): 257-75. 14. Kawabata, A. et al. (2011) Analysis of a Neutralizing Antibody for Human Herpesvirus 6B Reveals a Role for Glycoprotein Q1 in Viral Entry.
J Virol. 85: 12962-71. 15. Cornfine, S. et al. (2011) The kinesin KIF9 and reggie/flotillin proteins regulate matrix degradation by macrophage podosomes.
Mol Biol Cell. 22: 202-15. 16. Luo, S. et al. (2015) Contribution of N-linked glycans on HSV-2 gB to cell-cell fusion and viral entry.
Virology. 483: 72-82. 17. El Kasmi, I. & Lippé, R. (2015) Herpes simplex virus 1 gN partners with gM to modulate the viral fusion machinery.
J Virol. 89 (4): 2313-23. 18. Chia, R. et al. (2014) Phosphorylation of LRRK2 by casein kinase 1a regulates trans-Golgi clustering via differential interaction with ARHGEF7.
Nat Commun. 5: 5827. 19. Vorobyeva, A.G. et al. (2014) Cyclopamine modulates ?-secretase-mediated cleavage of amyloid precursor protein by altering its subcellular trafficking and lysosomal degradation.
J Biol Chem. 289 (48): 33258-74. 20. Wang Z et al. (2014) A newly identified myomegalin isoform functions in Golgi microtubule organization and ER-Golgi transport.
J Cell Sci. 127 (22): 4904-17. 21. Ikawa Y et al.(2015)In vitro functional correction of Hermansky-Pudlak Syndrome type-1 by lentiviral-mediated gene transfer.
Mol Genet Metab. 114 (1): 62-5. 22. Ioannou, M.S. et al. (2015) DENND2B activates Rab13 at the leading edge of migrating cells and promotes metastatic behavior.
J Cell Biol. 208 (5): 629-48. 23. Gottschalk, E.Y. & Meneses, P.I. (2015) A dual role for the non-receptor tyrosine kinase Pyk2 during the intracellular trafficking of human papillomavirus 16.
J Virol. pii: JVI.01183-15.1. Ponnambalam, S. et al. (1996) Primate homologues of rat TGN38: primary structure, expression and functional implications.
J. Cell Sci. 109: 675-685.

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Cat# ABD10057
(40 western blots)
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