|Application ||WB, IHC-F, IF|
|Calculated MW||51113 Da|
|Purification||Antisera to human TGN46 were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography.|
|Immunogen||Recombinant human TGN46|
|Shelf Life||18 months from date of despatch.|
|Other Names||Trans-Golgi network integral membrane protein 2, TGN38 homolog, TGN46, TGN48, Trans-Golgi network protein TGN51, TGOLN2, TGN46, TGN51|
|Target/Specificity||Sheep anti-Human TGN46 antibody recognizes Trans-Golgi network integral membrane protein 2 (TGOLN2), also known as TGN38 homolog, TGN46, TGN48 or Trans-Golgi network protein TGN51. TGN46 is a 437 amino acid glycoprotein localized to the Trans-Golgi network. TGN46 has been reported as being the best available marker for human trans-Golgi network.TGN46 is a heavily glycosylated protein of around 110-120 kDa. Multiple isoforms of TGN46 are generated by alternative splicing difffering in sequence at the C-terminal protion. Sheep anti-Human TGN46 antibody is expeced to recognize all identified isoforms.|
|Preservative & Stabilisers||0.02% Sodium Azide (NaN3); 1% Bovine Serum Albumin; 25% Glycerol|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human TGN46 Antibody (Serum) is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Prescott, A.R. et al. (1997) Distinct compartmentalization of TGN46 and beta 1, 4-galactosyltransferase in HeLa cells.Eur. J. Cell Biol. 72: 238-246. 3. Vuillier, F. et al. (2005) Lower levels of surface B-cell-receptor expression in chronic lymphocytic leukemia are associated with glycosylation and folding defects of the mu and CD79a chains.Blood. 105: 2933-2940. 4. Edwards, T.L. et al. (2009) Endogenous spartan (SPG20) is recruited to endosomes and lipid droplets and interacts with the ubiquitin E3 ligases AIP4 and AIP5.Biochem. J. 423: 31-39. 5. Roberts, R.C. et al. (2010) Mistargeting of SH3TC2 away from the recycling endosome causes Charcot-Marie-Tooth disease type 4C. Hum Mol Genet.19: 1009-18. 6. Hauser, H. et al. (2010) HIV-1 Vpu and HIV-2 Env counteract BST-2/tetherin by sequestration in a perinuclear compartment. Retrovirology. 7: 51. 7. Oliver, S.L. et al. (2011) Mutagenesis of varicella-zoster virus glycoprotein I (gI) identifies a cysteine residue critical for gE/gI heterodimer formation, gI structure, and virulence in skin cells.J Virol. 85 (9): 4095-110. 8. Berarducci, B. et al. (2006) Essential functions of the unique N-terminal region of the varicella-zoster virus glycoprotein E ectodomain in viral replication and in the pathogenesis of skin infection.J Virol. 80: 9481-96. 9. Sadaoka, T. et al. (2010) Characterization of the varicella-zoster virus ORF50 gene, which encodes glycoprotein M.J Virol. 84: 3488-502. 10. Vleck, S.E. et al. (2010) Anti-glycoprotein H antibody impairs the pathogenicity of varicella-zoster virus in skin xenografts in the SCID mouse model.J Virol. 84: 141-52. 11. Esk, C. et al. (2010) The clathrin heavy chain isoform CHC22 functions in a novel endosomal sorting step.J Cell Biol. 188: 131-44. 12. Cheng, S.B. et al. (2011) Down-modulation of the G-protein-coupled Estrogen Receptor, GPER, from the Cell Surface Occurs via a trans-Golgi-Proteasome Pathway.J Biol Chem. 286: 22441-55. 13. Fairn, G.D. et al. (2011) High-resolution mapping reveals topologically distinct cellular pools of phosphatidylserine.J Cell Biol. 194 (2): 257-75. 14. Kawabata, A. et al. (2011) Analysis of a Neutralizing Antibody for Human Herpesvirus 6B Reveals a Role for Glycoprotein Q1 in Viral Entry.J Virol. 85: 12962-71. 15. Cornfine, S. et al. (2011) The kinesin KIF9 and reggie/flotillin proteins regulate matrix degradation by macrophage podosomes.Mol Biol Cell. 22: 202-15. 16. Luo, S. et al. (2015) Contribution of N-linked glycans on HSV-2 gB to cell-cell fusion and viral entry.Virology. 483: 72-82. 17. El Kasmi, I. & Lippé, R. (2015) Herpes simplex virus 1 gN partners with gM to modulate the viral fusion machinery.J Virol. 89 (4): 2313-23. 18. Chia, R. et al. (2014) Phosphorylation of LRRK2 by casein kinase 1a regulates trans-Golgi clustering via differential interaction with ARHGEF7.Nat Commun. 5: 5827. 19. Vorobyeva, A.G. et al. (2014) Cyclopamine modulates ?-secretase-mediated cleavage of amyloid precursor protein by altering its subcellular trafficking and lysosomal degradation.J Biol Chem. 289 (48): 33258-74. 20. Wang Z et al. (2014) A newly identified myomegalin isoform functions in Golgi microtubule organization and ER-Golgi transport.J Cell Sci. 127 (22): 4904-17. 21. Ikawa Y et al.(2015)In vitro functional correction of Hermansky-Pudlak Syndrome type-1 by lentiviral-mediated gene transfer.Mol Genet Metab. 114 (1): 62-5. 22. Ioannou, M.S. et al. (2015) DENND2B activates Rab13 at the leading edge of migrating cells and promotes metastatic behavior.J Cell Biol. 208 (5): 629-48. 23. Gottschalk, E.Y. & Meneses, P.I. (2015) A dual role for the non-receptor tyrosine kinase Pyk2 during the intracellular trafficking of human papillomavirus 16.J Virol. pii: JVI.01183-15.1. Ponnambalam, S. et al. (1996) Primate homologues of rat TGN38: primary structure, expression and functional implications.J. Cell Sci. 109: 675-685.
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