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Anti-AIF (aa517-531) Antibody

Rabbit Anti-Human Polyclonal Antibody

  • WB - Anti-AIF (aa517-531) Antibody  ABD10064
    Western blot analysis of whole cell lysates from K562 (A), rat heart (B) and mouse heart (C) probed with Rabbit anti Human AIF
  • IHC-P - Anti-AIF (aa517-531) Antibody  ABD10064
    Staining of human retina with Rabbit anti Human AIF at 10 µg/ml. Formalin fixed paraffin processed tissue
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O95831
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 66901 Da
Additional Information
Other Species Rat,M
Purification Antisera to AIF were raised by repeated immunisation of rabbit with highly purified antigen. Purified IgG was prepared from whole serum by ion exchange chromatography.
Immunogen Peptide corresponding to amino acids 517 - 531 of human AIF.
Shelf Life 18 months from date of despatch.
Gene ID 9131
Other Names Apoptosis-inducing factor 1, mitochondrial, 1.1.1.-, Programmed cell death protein 8, AIFM1, AIF, PDCD8
Target/Specificity Rabbit anti-Human AIF antibody recognizes human Apoptosis-inducing factor 1, mitochondrial, also known as Programmed cell death protein 8 or apoptosis inducing factor (AIF). AIF is a 613 amino acid member of the FAD-dependent oxidoreductase family.Rabbit anti-Human AIF antibody reacts with an internal region of AIF (within the region aa 517-531. AIF is widely expressed in human tissues and localizes in the mitochondria. AIF translocates to the nucleus upon induction of apoptosis. Multiple isoforms of AIF are generated by alternative splicing. Rabbit anti-Human AIF antibody recognizes isoforms 1, 2,3 and 5. It is not expected to recognize either isoforms 4 or 6 as they lack the immunogen sequence.Mutations in the AIFM1 can result in various disease conditions including COXPD6andCowchock syndrome.
Preservative & Stabilisers 0.02% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-AIF (aa517-531) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Susin. S.A. et al. (1999) Molecular characterization of mitochondrial apoptosis - inducing factor.
Nature. 397: 441-6.

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Cat# ABD10064
(40 western blots)
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