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Anti-Rat Calcitonin Receptor Antibody

Rabbit Anti-Rat Polyclonal Antibody

  • IF - Anti-Rat Calcitonin Receptor Antibody  ABD10067
    Published customer image:Rabbit anti Rat Calcitonin Receptor antibody used for the detection of calcitonin receptor (CTR) on murine myofibres by immunofluorescence.Image caption:Caveolin-1, integrin α7 and CTR are expressed by Pax7+ve quiescent satellite cells in their niche on the myofibre. Satellite cells in their niche on the surface of freshly isolated EDL myofibres were co-immunostained for Pax7 (green) and either caveolin-1 (a - red), integrin α7 (b - red) or CTR (c - red). All three proteins were expressed in the plasma membrane of Pax7+ve satellite cells (arrowed), clearly revealing their bi-polar morphology. Myofibres were also examined from the masseter of the jaw, and again Pax7+ve satellite cells expressed all three markers (caveolin-1 shown as an example in d). All nuclei were counterstained with DAPI (blue), revealing the presence of the myonuclei of the myofibres on which the satellite cells were located. Scale bar represents 20 μm.From: Gnocchi VF, White RB, Ono Y, Ellis JA, Zammit PS Further Characterisation of the Molecular Signature of Quiescent and Activated Mouse Muscle Satellite Cells.PLoS ONE 4: e5205.
  • IF - Anti-Rat Calcitonin Receptor Antibody  ABD10067
    Published customer image:Rabbit anti Rat Calcitonin Receptor antibody used for the detection of calcitonin receptor on murine myofibres by immunofluorescence.Image caption:Jagged-1 is induced when satellite cells are activated. EDL myofibres were cultured in suspension for 48 hrs in plating medium to elicit the activation and proliferation of satellite cells . Activated and proliferating satellite cells were identified by immunostaining for MyoD , and Caveolin-1 (a - red), integrin α7 (b - red) and CTR (c - red) remained expressed in the plasma membrane of activated satellite cells. The continued presence of both emerin (red, d) and lamin A/C (green, e) in the nuclear envelope of activated satellite cells expressing MyoD (green in d, red in e) was also noted. Moreover, we found that the Notch ligand Jagged-1 was induced upon activation, in MyoD+ve satellite cells (f). Nuclei were counterstained with DAPI . Scale bar represents 20 μm.From: Gnocchi VF, White RB, Ono Y, Ellis JA, Zammit PS Further Characterisation of the Molecular Signature of Quiescent and Activated Mouse Muscle Satellite Cells.PLoS ONE 4(4): e5205.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P32214
Reactivity Rat
Host Rabbit
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 60292 Da
Additional Information
Other Species M,H,Fish,Atlantic Stingray
Purification Antisera to calcitonin receptor were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography on Protein A.
Immunogen Synthetic peptide derived from a C-terminal sequence of rat calcitonin receptor.
Shelf Life 12 months from date of reconstitution.
Gene ID 116506
Other Names Calcitonin receptor, CT-R, C1A/C1B, Calcr
Target/Specificity Rabbit anti-Rat Calcitonin Receptor antibody recognizes the calcitonin receptor in human, mouse and rat. The calcitonin receptor is a seven transmembrane domain cell surface protein which is coupled to G protein messenger systems. Rabbit anti-Rat Calcitonin Receptor antibody recognizes an epitope within the cytoplasmic domain that is common to both C1a and C1b rat isoforms.Calcitonin receptor is expressed in kidney, central and peripheral nervous systems and in osteoclasts. It is also expressed in a number of tissues during the development of the embryo and may be important in foetal morphogenesis.
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Rat Calcitonin Receptor Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)

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1. Wookey, P. J. et al. (2008) Calcitonin receptor immunoreactivity associated with specific cell types in diseased radial and internal mammary arteries.
Histopathology 52: 605-612. 2. Tolcos, M. et al. (2003) Ontogeny of calcitonin receptor mRNA and protein in the developing central nervous system of the rat.
J. Comp. Neurol. 456: 29 - 38. 3. Tikellis, C. et al. (2003) Calcitonin receptor isoforms expressed in the developing rat kidney.
Kidney Int. 63: 416-26. 4. Gnocchi, V.F. et al. (2009) Further characterisation of the molecular signature of quiescent and activated mouse muscle satellite cells.
PLoS One. 4(4):e5205. 5. Becskei, C. et al. (2004) Immunohistochemical mapping of calcitonin receptors in the adult rat brain.
Brain Res. 1030: 221-33. 6. Gooi, J.H. et al. (2010) Calcitonin impairs the anabolic effect of PTH in young rats and stimulates expression of sclerostin by osteocytes.
Bone. 46: 1486-97. 7. Gelsleichter, J.J. (2006) MCAA: Development of laboratory skills in molecular biology to examine patterns of calcitonin and calcitonin receptor gene expression in cartilaginous fishes.
Mote technical report 1392. 8. Wookey, P.J. et al. (2012) Transient expression of the calcitonin receptor by enteric neurons of the embryonic and early post-natal mouse.
Cell Tissue Res. 347: 311-7. 9. Mourikis, P. et al. (2012) Cell-autonomous Notch activity maintains the temporal specification potential of skeletal muscle stem cells.
Development. 139: 4536-48. 10. Park, J.K. et al. (2012) Increased generation of TRAP expressing multinucleated giant cells in patients with granulomatosis with polyangiitis.
PLoS One. 7: e42659. 11. Le Roux, I. et al. (2015) Numb is required to prevent p53-dependent senescence following skeletal muscle injury.
Nat Commun. 6: 8528. 12. Almeida, C. et al. (2016) Muscle satellite cells: exploring the basic biology to rule them
Stem Cells Int. 24 Jan [Epub ahead of print] 13. Yamaguchi, M. et al. (2015) Calcitonin Receptor Signaling Inhibits Muscle Stem Cells from Escaping the Quiescent State and the Niche.
Cell Rep. 13 (2): 302-14. 14. Wookey, P.J. (2009) A Review of Calcitonin Receptor Expression in Embryonic, Foetal and Adult Tissues, with an Hypothesis on the Connection Between Expression During Foetal Development and Disease
The Open Zoology Journal 2: 53-61.

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$ 527.00
$ 88.00
Cat# ABD10067
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