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Anti-Endonuclease G Antibody

Rabbit Anti-Human Polyclonal Antibody

  • WB - Anti-Endonuclease G Antibody  ABD10069
    Western blot analysis of whole cell lysates from mouse 3T3 (M) and Human HepG2 (H) cells probed with Rabbit anti endonuclease G
  • IHC-P - Anti-Endonuclease G Antibody  ABD10069
    Staining of formalin fixed, paraffin processed human pancreas with Rabbit anti Human EndoG at 15 µg/ml.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q14249
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 32620 Da
Additional Information
Purification Antisera to endonuclease G were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Immunogen Synthetic peptide GGPRGPGELAKYGLP corresponding to amino acids 55 - 70 of endonuclease G.
Shelf Life 18 months from date of despatch.
Gene ID 2021
Other Names Endonuclease G, mitochondrial, Endo G, 3.1.30.-, ENDOG
Target/Specificity Rabbit anti-Endonuclease G antibody recognizes human endonuclease G, also known as endoG. Endo G is a 297 amino acid, including a 48 amino acid transit peptide ~36 kDa mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis.EndoG is reported to act independently of caspases and may represent a caspase independent apoptotic pathway initiated from the mitochondria (Liet al.2001).
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Endonuclease G Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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1. Colado, E. et al. (2011) Zalypsis has in vitro activity in acute myeloid blasts and leukemic progenitor cells through the induction of a DNA damage response.
Haematologica. 96: 687-95.1. Li, L.Y. et al. (2001) Endonuclease G is an apoptotic DNase when released from mitochondria.
Nature. 412: 95 - 99.

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Cat# ABD10069
(40 western blots)
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