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Anti-Caspase-7 (C-Terminal) Antibody

Rabbit Anti-Human Polyclonal Antibody

  • IHC - Anti-Caspase-7 (C-Terminal) Antibody  ABD10079
    Immunohistochemical staining of human skeletal muscle with Rabbit anti Human caspase-7
  • WB - Anti-Caspase-7 (C-Terminal) Antibody  ABD10079
    Western blot analysis of human skeletal muscle probed with Rabbit anti Human caspase-7 at 0.5(A), 1(B) and 2(C) ug/ml
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P55210
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 34277 Da
Additional Information
Other Species M,Rat
Purification Antisera to human Caspase-7 (CT) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Immunogen A 16 amino acid peptide from human Caspase-7 carboxy-terminus
Shelf Life 18 months from date of despatch.
Gene ID 840
Other Names Caspase-7, CASP-7,, Apoptotic protease Mch-3, CMH-1, ICE-like apoptotic protease 3, ICE-LAP3, Caspase-7 subunit p20, Caspase-7 subunit p11, CASP7, MCH3
Target/Specificity ABD10079 recognises an epitope within the C-terminal region (CT) of Caspase-7, a 35kDa cysteine protease, otherwise known as ICE-like Apoptotic Protease 3 (ICE-LAP3). Caspase-7, a member of the ICE/Ced-3 subfamily, is an executioner caspase which undergoes proteolytic cleavage of its precursor to form active p12 and p20 subunits. Evidence that activation of Caspase-7 occurs during cell death induced by the cytokine death receptors Fas/APO-1 and the receptor of tumour necrosis factor (TNFR-1), coupled with the fact that granzyme-B activated Caspase-7 cleaves the nuclear enzyme poly (ADP-ribose) polymerase (PARP), suggests an important role for Caspase-7 in both cytokine-mediated and Granzyme-B mediated apoptosis pathways.
Preservative & Stabilisers 0.02% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Caspase-7 (C-Terminal) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Duan, H. et al. (1996) ICE-LAP3, a novel mammalian homologue of the Caenorhabditis elegans cell death protein Ced-3 is activated during Fas- and tumor necrosis factor-induced apoptosis.
J. Biol Chem. 271: 1621 - 1625.2. Juan, T.S. et al. (1997) Identification and mapping of Casp7, a cysteine protease resembling CPP32 beta, interleukin-1 beta converting enzyme, and CED-3.
Genomics. 40: 86-93.3. Cohen, G.M. (1997) Caspases: the executioners of apoptosis.
J. Biochem. 326: 1 - 16.

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Cat# ABD10079
(40 western blots)
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