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Anti-Human APOBEC3G (N-Terminal) Antibody

Rabbit Anti-Human Polyclonal Antibody

  • WB - Anti-Human APOBEC3G (N-Terminal) Antibody  ABD10083
    Western blot analysis of whole cell lysate from Caco-2 human colorectal carcinoma cell line probed with Rabbit anti Human APOBEC3G
  • IHC - Anti-Human APOBEC3G (N-Terminal) Antibody  ABD10083
    Immunohistochemical staining of human spleen with Rabbit anti Human AOPBEC3G
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9HC16
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 46408 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography.
Immunogen A 15 amino acid peptide located near human APOBEC3G amino-terminus.
Shelf Life 18 months from date of despatch.
Gene ID 60489
Other Names DNA dC->dU-editing enzyme APOBEC-3G, 3.5.4.-, APOBEC-related cytidine deaminase, APOBEC-related protein, ARCD, APOBEC-related protein 9, ARP-9, CEM-15, CEM15, Deoxycytidine deaminase, A3G, APOBEC3G
Target/Specificity ABD10083 recognises an epitope within the N-terminal region (NT) of APOBEC3G (apolipoprotein B mRNA-editing, enzyme-catalytic, polypeptide-like 3G), a 46kDa multi-isoform endogenous deoxycytidine deaminase, which plays a major role in the defence of T lymphocytes against retroviral infection.The incorporation of APOBEC3G into HIV-1 virion DNA, results in the deamination of dC to dU in minus-strand viral DNA during reverse transcription, thereby generating G to A hypermutations in the newly-synthesised viral DNA. The effect of APOBEC3G is counteracted by the HIV protein Vif, thought to induce the degradation of APOBEC3G through a ubiquitin-dependant proteasomal pathway.ABD10083 also detects the APOBEC3F isoform (apolipoprotein B mRNA-editing, enzyme-catalytic, polypeptide-like 3F).
Preservative & Stabilisers 0.02% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human APOBEC3G (N-Terminal) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Goncalves, J. and Santa-Marta, M. (2004) HIV-1 Vif and APOBEC3G: multiple roads to one goal..
Retrovirology. 28(1): 1.2. Chiu, Y-L. et al. (2005) Cellular APOBEC3G restricts HIV-1 infection in resting CD4+ T cells.
Nature. 435: 108-114.3. Zhang, H. et al. (2003) The cytidine deaminase CEM15 induces hypermutation in newly synthesized HIV-1 DNA.
Nature. 424: 94-98.

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Cat# ABD10083
(40 western blots)
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