|Application ||WB, IHC-P|
|Calculated MW||46408 Da|
|Purification||Purified IgG prepared by affinity chromatography.|
|Immunogen||A 15 amino acid peptide located near human APOBEC3G amino-terminus.|
|Shelf Life||18 months from date of despatch.|
|Other Names||DNA dC->dU-editing enzyme APOBEC-3G, 3.5.4.-, APOBEC-related cytidine deaminase, APOBEC-related protein, ARCD, APOBEC-related protein 9, ARP-9, CEM-15, CEM15, Deoxycytidine deaminase, A3G, APOBEC3G|
|Target/Specificity||ABD10083 recognises an epitope within the N-terminal region (NT) of APOBEC3G (apolipoprotein B mRNA-editing, enzyme-catalytic, polypeptide-like 3G), a 46kDa multi-isoform endogenous deoxycytidine deaminase, which plays a major role in the defence of T lymphocytes against retroviral infection.The incorporation of APOBEC3G into HIV-1 virion DNA, results in the deamination of dC to dU in minus-strand viral DNA during reverse transcription, thereby generating G to A hypermutations in the newly-synthesised viral DNA. The effect of APOBEC3G is counteracted by the HIV protein Vif, thought to induce the degradation of APOBEC3G through a ubiquitin-dependant proteasomal pathway.ABD10083 also detects the APOBEC3F isoform (apolipoprotein B mRNA-editing, enzyme-catalytic, polypeptide-like 3F).|
|Preservative & Stabilisers||0.02% Sodium Azide (NaN3)|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human APOBEC3G (N-Terminal) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Goncalves, J. and Santa-Marta, M. (2004) HIV-1 Vif and APOBEC3G: multiple roads to one goal..Retrovirology. 28(1): 1.2. Chiu, Y-L. et al. (2005) Cellular APOBEC3G restricts HIV-1 infection in resting CD4+ T cells.Nature. 435: 108-114.3. Zhang, H. et al. (2003) The cytidine deaminase CEM15 induces hypermutation in newly synthesized HIV-1 DNA.Nature. 424: 94-98.
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