|Calculated MW||36053 Da|
|Purification||Purified IgG prepared by affinity chromatography.|
|Immunogen||A 16 amino acid peptide located near human GAPDH carboxy-terminus.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Glyceraldehyde-3-phosphate dehydrogenase, GAPDH, 18.104.22.168, Peptidyl-cysteine S-nitrosylase GAPDH, 2.6.99.-, GAPDH, GAPD|
|Target/Specificity||Rabbit anti-GAPDH (C-Terminal) antibody recognizes an epitope within the C-Terminal region (CT) of Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a glycolytic enzyme which plays a key role in energy production, and has also been implicated in numerous cellular processes.GAPDH is a homotetramer molecule consisting of four 36kDa subunits, constitutively expressed in most cells and tissues, and is responsible for the generation of energy during carbohydrate metabolism, catalysing the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate.The role of GAPDH as a multifunctional protein has emerged from many studies, confirming its involvement in critical nuclear pathways, apoptosis, membrane transport and fusion, DNA replication and repair and phosphotransferase activity. Furthermore, the ability of GAPDH to bind with high affinity to ß-amyloid precursor protein (Alzheimers disease) and selectively with CAG mutated proteins, including huntingtin (Huntingdons disease) and the androgen receptor (spinobulbar muscular atrophy), has focused many studies towards the role of GAPDH in the pathogenesis of neurodegenerative diseases (De Santo et al.2010).|
|Preservative & Stabilisers||0.02% Sodium Azide (NaN3)|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-GAPDH (C-Terminal) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. De Santo, C. et al. (2010) Invariant NKT cells modulate the suppressive activity of IL-10-secreting neutrophils differentiated with serum amyloid A.Nat Immunol. 11 (11): 1039-46. 2. Weeke-Klimp, A. et al. (2010) Epicardium-derived cells enhance proliferation, cellular maturation and alignment of cardiomyocytes.J Mol Cell Cardiol. 49: 606-16. 3. Deacon, K. and Knox, A.J. (2010) Endothelin-1 (ET-1) increases the expression of remodeling genes in vascular smooth muscle through linked calcium and cAMP pathways: role of a phospholipase A(2)(cPLA(2))/cyclooxygenase-2 (COX-2)/prostacyclin receptor-dependent autocrine loop.J Biol Chem. 285: 25913-27. 1. Mazzola, J.L. and Sirover, M.A (2001) Reduction of glyceraldehyde-3-phosphate dehydrogenase activity in Alzheimer’s disease and in Huntington’s disease fibroblasts.J. Neurochem. 76: 442-449.2. Sirover, M.A. (2005) New nuclear functions of the glycolytic protein, glyceraldehyde-3-phosphate dehydrogenase, in mammalian cells. J. Cell Biochem. 95: 45-52.
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