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Anti-Mouse CD90 Antibody, clone F7D5

Mouse Anti-Mouse Monoclonal Antibody

  • FC - Anti-Mouse CD90 Antibody, clone F7D5  ABD10086
    Published customer image:Mouse anti Mouse CD90 antibody, clone F7D5 used for T cell depletion of bone marrow and subsequent evaluation of chimerism by flow cytometry.Image caption:Donor chimerism and alloreactivity. a Percentage donor T cell chimerism in peripheral blood lymphocytes, determined by Thy1.1 (recipient) and Thy1.2 (donor) positivity. b percentage alloreactive CD3+Vß6+ T cells. Results are mean ± SEM from 5 animals in BMT+SPL+SRI and BMT only group and from 4 animals in BMT+SPL+vehicle group. Statistically significant differences are depicted with * (one-tailed p
  • FC - Anti-Mouse CD90 Antibody, clone F7D5  ABD10086
    Staining of mouse thymus cells with Mouse anti Mouse CD90 (Thy 1.2)
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P01831
Reactivity Mouse
Host Mouse
Clonality Monoclonal
Isotype IgM
Clone Names F7D5
Calculated MW 18080 Da
Additional Information
Purification IgM fraction prepared by ammonium sulphate precipitation
Shelf Life 18 months from date of despatch.
Gene ID 21838
Other Names Thy-1 membrane glycoprotein, Thy-1 antigen, CD90, Thy1, Thy-1
Target/Specificity Mouse anti-Mouse CD90 antibody, clone F7D5 recognizes the mouse Thy1.2 alloantigen, expressed by thymocytes and peripheral T lymphocytes. Clone F7D5 reacts with Thy1.2 mice such as CBA and BALB/C, but not with Thy1.1 mice eg. AKR and FUB.The antibody is particularly useful for removal of T lymphocytes from cell populations by complement mediated cytotoxicity (Lake, P et al.1979).Mouse anti-Mouse CD90 antibody, clone F7D5 is routinely tested in flow cytometry using mouse thymocytes.
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Mouse CD90 Antibody, clone F7D5 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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1. Lake, P. et al. (1979) Production and characterization of cytotoxic Thy-1 antibody-secreting hybrid cell lines. Detection of T cell subsets.
Eur J Immunol. 9 (11): 875-86. 2. Yoshida, K. et al. (2002) Evidence for shared recognition of a peptide ligand by a diverse panel of non-obese diabetic mice-derived, islet-specific, diabetogenic T cell clones.
Int Immunol. 14 (12): 1439-47. 3. de Vries-van der Zwan, A. et al. (1997) Specific tolerance induction and transplantation: a single-day protocol.
Blood. 89(7):2596-2601 4. Winzeler, A.M. et al. (2011) The lipid sulfatide is a novel myelin-associated inhibitor of CNS axon outgrowth.
J Neurosci. 31: 6481-92. 5. Unterlauft, J.D. et al. (2014) Enhanced survival of retinal ganglion cells is mediated by Müller glial cell-derived PEDF.
Exp Eye Res. pii: S0014-4835(14)00218-8. 6. Brown, R.L. et al. (2015) TRPM3 Expression in Mouse Retina.
PLoS One. 10: e0117615. 7. Hanafusa, T. et al. (1988) Induction of insulitis by adoptive transfer with L3T4+Lyt2- T-lymphocytes in T-lymphocyte-depleted NOD mice.
Diabetes. 37: 204-8. 8. Billiau, A.D. et al. (2003) Transient expansion of Mac1+Ly6-G+Ly6-C+ early myeloid cells with suppressor activity in spleens of murine radiation marrow chimeras: possible implications for the graft-versus-host and graft-versus-leukemia reactivity of donor lymphocyte infusions.
Blood. 102: 740-8. 9. Raeber, A.J. et al. (1999) PrP-dependent association of prions with splenic but not circulating lymphocytes of scrapie-infected mice.
EMBO J. 18: 2702-6. 10. Wang, X. et al. (2001) Functional soluble CD100/Sema4D released from activated lymphocytes: possible role in normal and pathologic immune responses
Blood. 2001 Jun 1;97(: 3498-504. 11. Billiau AD et al. (2002) Crucial role of timing of donor lymphocyte infusion in generating dissociated graft-versus-host and graft-versus-leukemia responses in mice receiving allogeneic bone marrow transplants.
Blood. 100 (5): 1894-902. 12. Ishikawa N et al. (1998) Early cytokine responses during intestinal parasitic infections.
Immunology. 93 (2): 257-63. 13. Ishigaki H et al. (2006) Preparation and functional analysis of tumor-infiltrating stroma cells using bone marrow chimera mice.
Microbiol Immunol. 50 (8): 655-62. 14. Logan GJ et al. (2004) CD4 expression on EL4 cells as an epiphenomenon of retroviral transduction and selection.
Immunol Cell Biol. 82 (2): 132-5. 15. Gobin V et al. (2013) Fluoxetine reduces murine graft-versus-host disease by induction of T cell immunosuppression.
J Neuroimmune Pharmacol. 8 (4): 934-43. 16. van Pel M et al. (2003) Towards a myeloablative regimen with clinical potential: I. Treosulfan conditioning and bone marrow transplantation allow induction of donor-specific tolerance for skin grafts across full MHC barriers.
Bone Marrow Transplant. 32 (1): 15-22. 17. Oosterwegel MA et al. (1999) The role of CTLA-4 in regulating Th2 differentiation.
J Immunol. 163 (5): 2634-9. 18. Vadivelu S et al. (2015) NG2+ Progenitors Derived From Embryonic Stem Cells Penetrate Glial Scar and Promote Axonal Outgrowth Into White Matter After Spinal Cord Injury.
Stem Cells Transl Med. pii: sctm.2014-0107.

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Cat# ABD10086
(40 western blots)
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