|Application ||FC, CHIP|
|Calculated MW||18080 Da|
|Purification||IgM fraction prepared by ammonium sulphate precipitation|
|Shelf Life||18 months from date of despatch.|
|Other Names||Thy-1 membrane glycoprotein, Thy-1 antigen, CD90, Thy1, Thy-1|
|Target/Specificity||Mouse anti-Mouse CD90 antibody, clone F7D5 recognizes the mouse Thy1.2 alloantigen, expressed by thymocytes and peripheral T lymphocytes. Clone F7D5 reacts with Thy1.2 mice such as CBA and BALB/C, but not with Thy1.1 mice eg. AKR and FUB.The antibody is particularly useful for removal of T lymphocytes from cell populations by complement mediated cytotoxicity (Lake, P et al.1979).Mouse anti-Mouse CD90 antibody, clone F7D5 is routinely tested in flow cytometry using mouse thymocytes.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Mouse CD90 Antibody, clone F7D5 is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Lake, P. et al. (1979) Production and characterization of cytotoxic Thy-1 antibody-secreting hybrid cell lines. Detection of T cell subsets.Eur J Immunol. 9 (11): 875-86. 2. Yoshida, K. et al. (2002) Evidence for shared recognition of a peptide ligand by a diverse panel of non-obese diabetic mice-derived, islet-specific, diabetogenic T cell clones.Int Immunol. 14 (12): 1439-47. 3. de Vries-van der Zwan, A. et al. (1997) Specific tolerance induction and transplantation: a single-day protocol.Blood. 89(7):2596-2601 4. Winzeler, A.M. et al. (2011) The lipid sulfatide is a novel myelin-associated inhibitor of CNS axon outgrowth.J Neurosci. 31: 6481-92. 5. Unterlauft, J.D. et al. (2014) Enhanced survival of retinal ganglion cells is mediated by Müller glial cell-derived PEDF.Exp Eye Res. pii: S0014-4835(14)00218-8. 6. Brown, R.L. et al. (2015) TRPM3 Expression in Mouse Retina.PLoS One. 10: e0117615. 7. Hanafusa, T. et al. (1988) Induction of insulitis by adoptive transfer with L3T4+Lyt2- T-lymphocytes in T-lymphocyte-depleted NOD mice.Diabetes. 37: 204-8. 8. Billiau, A.D. et al. (2003) Transient expansion of Mac1+Ly6-G+Ly6-C+ early myeloid cells with suppressor activity in spleens of murine radiation marrow chimeras: possible implications for the graft-versus-host and graft-versus-leukemia reactivity of donor lymphocyte infusions.Blood. 102: 740-8. 9. Raeber, A.J. et al. (1999) PrP-dependent association of prions with splenic but not circulating lymphocytes of scrapie-infected mice.EMBO J. 18: 2702-6. 10. Wang, X. et al. (2001) Functional soluble CD100/Sema4D released from activated lymphocytes: possible role in normal and pathologic immune responsesBlood. 2001 Jun 1;97(: 3498-504. 11. Billiau AD et al. (2002) Crucial role of timing of donor lymphocyte infusion in generating dissociated graft-versus-host and graft-versus-leukemia responses in mice receiving allogeneic bone marrow transplants.Blood. 100 (5): 1894-902. 12. Ishikawa N et al. (1998) Early cytokine responses during intestinal parasitic infections.Immunology. 93 (2): 257-63. 13. Ishigaki H et al. (2006) Preparation and functional analysis of tumor-infiltrating stroma cells using bone marrow chimera mice.Microbiol Immunol. 50 (8): 655-62. 14. Logan GJ et al. (2004) CD4 expression on EL4 cells as an epiphenomenon of retroviral transduction and selection.Immunol Cell Biol. 82 (2): 132-5. 15. Gobin V et al. (2013) Fluoxetine reduces murine graft-versus-host disease by induction of T cell immunosuppression.J Neuroimmune Pharmacol. 8 (4): 934-43. 16. van Pel M et al. (2003) Towards a myeloablative regimen with clinical potential: I. Treosulfan conditioning and bone marrow transplantation allow induction of donor-specific tolerance for skin grafts across full MHC barriers.Bone Marrow Transplant. 32 (1): 15-22. 17. Oosterwegel MA et al. (1999) The role of CTLA-4 in regulating Th2 differentiation.J Immunol. 163 (5): 2634-9. 18. Vadivelu S et al. (2015) NG2+ Progenitors Derived From Embryonic Stem Cells Penetrate Glial Scar and Promote Axonal Outgrowth Into White Matter After Spinal Cord Injury.Stem Cells Transl Med. pii: sctm.2014-0107.
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