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Anti-Human CD49e Antibody, clone JBS5

Mouse Anti-Human Monoclonal Antibody

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-F, FC, IP, Func
Primary Accession P08648
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names JBS5
Calculated MW 114536 Da
Additional Information
Other Species Dog
Purification Purified IgG prepared by affinity chromatography on Protein G.
Shelf Life 18 months from date of despatch.
Gene ID 3678
Other Names Integrin alpha-5, CD49 antigen-like family member E, Fibronectin receptor subunit alpha, Integrin alpha-F, VLA-5, CD49e, Integrin alpha-5 heavy chain, Integrin alpha-5 light chain, ITGA5, FNRA
Target/Specificity Mouse anti-Human CD49e antibody, clone JBS5 recognizes the Alpha 5 integrin, also known as CD49e, VLA-5, Fibronectin receptor subunit alpha or Integrin alpha-F. CD49e is a 1049 amino acid ~130kDa single pass type-1 transmembrane glycoprotein containing seven FG-GAP repeats. Mouse anti-human CD49e antibody, clone JBS5 been shown to stimulate homotypic cellular aggregation of alpha 5 beta 1 positive lymphoid cell lines (Shenet al.1991). It also inhibits the adherence of cells to fibronectin.Removal of sodium azide is recommended prior to use in functional assays.
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human CD49e Antibody, clone JBS5 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Shen, C. X. et al. (1991) Antibodies to different members of the beta 1 (CD29) Integrins induce homotypic and heterotypic cellular aggregation.
Cell Immunol. 138: 216-228. 2. McCarty, O.J. et al. (2004) Evaluation of the role of platelet integrins in fibronectin-dependent spreading and adhesion.
J Thromb Haemost. 2: 1823-1833. 3. Bax, D.V. et al. (2004) Integrin alpha5beta1 and ADAM-17 interact in vitro and co-localize in migrating HeLa cells.
J Biol Chem. 279: 22377-22386. 4. Gassmann, P. et al. (2009) CXCR4 regulates the early extravasation of metastatic tumor cells in vivo.
Neoplasia. 11: 651-61. 5. Yao, H. et al. (2010) Increased potency of the PHSCN dendrimer as an inhibitorof human prostate cancer cell invasion, extravasation, and lung colony formation.
Clin Exp Metastasis 27:173–84. 6. Gassmann, P. et al (2010) In vivo tumor cell adhesion in the pulmonary microvasculature is exclusively mediated by tumor cell--endothelial cell interaction.
BMC Cancer. 10: 177. 7. Orazizadeh, M. et al. (2008) CD47 associates with alpha 5 integrin and regulates responses of human articular chondrocytes to mechanical stimulation in an in vitro model.
Arthritis Res Ther. 10: R4.

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Cat# ABD10092
(40 western blots)
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