|Application ||WB, IHC-P, E|
|Calculated MW||33845 Da|
|Purification||Purified IgM prepared by gel filtration from tissue culture supernatant|
|Immunogen||Crude human synaptic immunoprecipitate.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Synaptophysin, Major synaptic vesicle protein p38, SYP|
|Target/Specificity||Mouse anti-Human Synaptophysin antibody, clone SP15 recognizes human synaptophysin, also known as Major synaptic vesicle protein p38. Synaptophysin is a 313 amino acid synaptic vesicle associated protein of ~38kDa containing a single marvel domain. Synaptophysin expression is ubiquitous in synaptic vesicle membranes where it is the most abundant protein and is involved in the regulation of endocytosis (Kwonet al.2011) Loss and atypical expression of synaptophysin is noted in conditions such as Huntindon's disease (Goto and Hirano 1990) and Alzheimer's disease (Masliahet al.1989). Mutations in the synaptophysin gene have been identified and lead to a condition, Mental retardation, X-linked 96 (Tarpeyet al.2009).Mouse anti-human synaptophysin antibody, clone SP15 has been used for the detection of synaptophysin by immunofluorescence, immunohistochemistry and western blotting on murine (Vitryet al.2009), marmoset (Ramirezet al.2001), rat (Barret al.2004), lapine (Roheret al.2000) and human tissues (Beachet al.1997).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human Synaptophysin Antibody, clone SP15 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Honer, W.G. et al. (1994) Hippocampal synaptic pathology in patients with temporal lobe epilepsy.Acta Neuropathol. 87: 202-210. 2. Honer, W.G. et al. (1992) Regional synaptic pathology in Alzheimer's disease.Neurobiol. Aging. 13: 375-382. 3. Honer, W.G. et al. (1993) Human synaptic proteins with a heterogeneous distribution in cerebellum and visual cortex.Brain Res. 609: 9-20. 4. Honer, W.G. et al. (1992) Characterization of a synaptic antigen of interest in neuropsychiatric illness.Biol. Psychiatry 31: 147-158. 5. Honer, W.G. et al. (1989) Monoclonal antibodies to study the brain in schizophrenia.Brain Res. 500: 379-383. 6. Masliah, E. et al. (1994) Topographical distribution of synaptic-associated proteins in the neuritic plaques of Alzheimer's disease hippocampus.Acta Neuropathol. 87: 135-142. 7. Simpson, I.A. et al. (1994) Decreased concentrations of GLUT1 and GLUT3 glucose transporters in the brains of patients with Alzheimer's disease.Ann Neurol. 35: 546-551. 8. Wakabayashi, K. et al. (1994) Synapse alterations in the hippocampalentorhinal formation in Alzheimer's disease with and without Lewy body disease. Brain Research 667: 24-32. 9. Dickson, D.W. et al. (1994) Hippocampal sclerosis: a common pathological feature of dementia in very old (> or = 80 years of age) humans. Acta Neuropathologica 88: 212-221. 10. Dickson, D.W. et al. (1995) Correlations of synaptic and pathological markers with cognition of the elderly.Neurobiol Aging 16:285-98. 11. Vitry, S. et al. (2010) Enhanced degradation of synaptophysin by the proteasome in mucopolysaccharidosis type IIIB.Mol Cell Neurosci. 41: 8-18. 12. Khan, O.H. et al. (2006) Brain damage in neonatal rats following kaolin induction of hydrocephalus.Exp Neurol. 200 (2): 311-20. 13. Del Bigio, M.R. et al. (2003) Chronic hydrocephalus in rats and humans: white matter loss and behavior changes.Ann Neurol. 53 (3): 337-46.
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