|Application ||IHC-P, IHC-F|
|Calculated MW||115378 Da|
|Purification||Purified IgG prepared by hydrophobic interaction chromatography from tissue culture supernatant|
|Immunogen||Triton X-100-insoluble rat brain protein.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Neurofilament heavy polypeptide, NF-H, 200 kDa neurofilament protein, Neurofilament triplet H protein, Nefh, Nfh|
|Target/Specificity||Mouse anti-Rat Neurofilament 200 kDa antibody, clone RT97 recognizes the 200 kDa neurofilament protein in a range of species. Mouse anti-Rat Neurofilament 200 kDa antibody, clone RT97 stains various tumors including phaeochromocytoma, paraganglioma and ganglioneuroblastoma.Mouse anti-Rat Neurofilament 200k Da antibody, clone RT97 also recognizes a phosphorylation dependent epitope on fetal tau, Alzheimer's paired helical filament-tau and on microtubule associated protein 1B (MAP1B) by western blotting, however similar reactivity was not apparent in immunohistochemical studies (Johnstoneet al.1997).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Rat Neurofilament 200kDa Antibody, clone RT97 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Weber, K. et al. (1983) Neurofilaments, a subclass of intermediate filaments: structure and expression. C.S.H. Symp. Quant. Biol. 48: 717-729. 2. Anderton, B. H. et al. (1982) Monoclonal antibodies show that neurofibrillary tangles and neurofilaments share antigenic determinants. Nature 298: 84-86. 3. Johnstone, M. et al. (1997) The neurofilament antibody RT97 recognises a developmentally regulated phosphorylation epitope on microtubule associated protein 1B.J. Anat. 191: 229-244. 4. Tonge, D. A. et al. (1996) Expression of a developmentally regulated phosphorylated form of microtulate - associated protein B in sprouting and regenerating axons in vitro.Neuroscience. 73: 541 - 551. 5. Sann, H. et al. (1995) RT97: a marker for capsaicin-insensitive sensory endings in the rat skin.Cell Tissue Res. 282: 155 - 161. 6. Veeranna, et al. (2008) Neurofilament tail phosphorylation: identity of the RT-97 phosphoepitope and regulation in neurons by cross-talk among proline-directed kinases.J Neurochem. 2008 Oct;107(1): 35-49. 7. Logan, A. et al. (2006) Neurotrophic factor synergy is required for neuronal survival and disinhibited axon regeneration after CNS injury.Brain. 129: 490-502. 8. Lorber, B. et al. (2004) Stimulated regeneration of the crushed adult rat optic nerve correlates with attenuated expression of the protein tyrosine phosphatases RPTPalpha, STEP, and LAR.Mol Cell Neurosci. 27: 404-16. 9. Reynolds, J. et al. (2005) Age-dependent changes in Fibroblast growth factor 2 (FGF-2) expression in mouse cerebellar neurons.J Cell Mol Med. 9: 398-406. 10. Shin, D.H. et al. (2003) The correspondence between the labeling patterns of antibody RT97, neurofilaments, microtubule associated protein 1B and tau varies with cell types and development stages of chicken retina.Neurosci Lett. 342: 167-70. 11. Connolly ,A.A. et al. (1987) A comparative study of a silver stain and monoclonal antibody reactions on Alzheimer's neurofibrillary tangles.J Neurol Neurosurg Psychiatry. 50: 1221-4. 12. Doering, L.C. (1991) Transplantation of fetal CNS tissue into the peripheral nervous system: a model to study aberrant changes in the neuronal cytoskeleton.J Neural Transplant Plast. 2: 193-205. 13. McCarthy, P.W. et al. (1992) RT97- and calcitonin gene-related peptide-like immunoreactivity in lumbar intervertebral discs and adjacent tissue from the rat.J Anat. 180: 15-24. 14. Murphy, A. et al. (1993) Neurofilament expression in human T lymphocytes.Immunology. 79: 167-70. 15. Yabe, J.T. et al. (2001) Neurofilaments consist of distinct populations that can be distinguished by C-terminal phosphorylation, bundling, and axonal transport rate in growing axonal neurites.J Neurosci. 21: 2195-205. 16. Wang, S. et al. (2000) Progressive optic axon dystrophy and vacuslar changes in rd mice.Invest Ophthalmol Vis Sci. 41: 537-45. 17. Kuwamura, M. et al. (2004) Cerebral ganglioneuroblastoma in a golden retriever dog.Vet Pathol. 41: 282-4.
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