|Application ||IHC-P, IHC-F, IP, IH|
|Calculated MW||55233 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G|
|Immunogen||Synthetic peptide, CSRPSTSSRRRAISE, corresponding to amino acids 154-167 of human MDM2|
|Shelf Life||18 months from date of despatch.|
|Other Names||E3 ubiquitin-protein ligase Mdm2, 6.3.2.-, Double minute 2 protein, Hdm2, Oncoprotein Mdm2, p53-binding protein Mdm2, MDM2|
|Target/Specificity||Mouse anti-MDM2 antibody, clone SMP14 recognizes the 90kDa MDM2 protein, which forms a complex with p53 leading to p53 inhibition.MDM2 also acts to stimulate cell proliferation via its induction of transcription factors such as E2F1 and DPI.MDM2 is over expressed in a range of human malignancies, including soft tissue sarcomas, breast cancer and bladder cancer.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-MDM2 Antibody, clone SMP14 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Picksley, S.M. et al. (1994) Immunochemical analysis of the interaction of p53 with MDM2; - fine mapping of the MDM2 binding site on p53 using synthetic peptides.Oncogene 9: 2523-2529. 2. Martin, K. et al. (1995) Stimulation of E2F1/DP1 transcriptional activity by MDM2 oncoprotein.Nature 375: 691-694. 3. Gueven, N. et al. (2006) Defective p53 response and apoptosis associated with an ataxia-telangiectasia-like phenotype. Cancer Res. 66: 2907-12.
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