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>   home   >   Products   >   Primary Antibodies   >   Cardiovascular   >   Anti-Human CD162 Antibody, clone 3E2.25.5 (PL1)    

Anti-Human CD162 Antibody, clone 3E2.25.5 (PL1)

Mouse Anti-Human Monoclonal Antibody

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q14242
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names 3E2.25.5 (PL1)
Calculated MW 43201 Da
Additional Information
Purification Purified IgG prepared from ascites or tissue culture supernatant
Immunogen Human neutrophils.
Shelf Life 18 months from date of despatch.
Gene ID 6404
Other Names P-selectin glycoprotein ligand 1, PSGL-1, Selectin P ligand, CD162, SELPLG
Target/Specificity Mouse anti-Human CD162, clone 3E2.25.5 recognises the human CD162 cell surface glycoprotein, a 120 kDa molecule also known as P-Selectin Glycoprotein Ligand I (PSGL-1).CD162 is the major counter receptor for P-selectin and is involved in neutrophil attachment and rolling to endothelium. In peripheral blood CD162 is expressed by monocytes, granulocytes, T cells and some B cells.Mouse anti-Human CD162, clone 3E2.25.5 is a blocking antibody, inhibiting PSGL-1 and P-selectin interaction. Removal of the preservative is recommended prior to use in functional assays - The use of Slide-A-Lyser® dialysis cassette (EQU003) is recommended for this purpose.
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human CD162 Antibody, clone 3E2.25.5 (PL1) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Moore, K. et al. (1995) P-selectin glycoprotein ligand-1 mediates rolling of human neutrophils on P-selectin.
J. Cell. Biol. 128: 661-671. 2. Norman, K.E. et al. (1995) Leukocyte rolling in vivo is mediated by P-selectin glycoprotein ligand-1.
Blood 86: 4417-4421. 3. Laszik, Z. et al. (1996) P-selectin glycoprotein ligand-1 is broadly expressed in cells of myeloid, lymphoid, and dendritic lineage and in some nonhematopoietic cells.
Blood 88: 3010-3021. 4. Bournazos, S. et al. (2008) Monocyte functional responsiveness after PSGL-1-mediated platelet adhesion is dependent on platelet activation status.
Arterioscler Thromb Vasc Biol. 28: 1491-8. 5. Hayhoe, R.P. et al. (2006) Annexin 1 and its bioactive peptide inhibit neutrophil-endothelium interactions under flow: indication of distinct receptor involvement.
Blood. 107: 2123-30.

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Cat# ABD10103
(40 western blots)
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