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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Anti-Human Estrogen Receptor alpha Antibody, clone 6F11    

Anti-Human Estrogen Receptor alpha Antibody, clone 6F11

Mouse Anti-Human Monoclonal Antibody

     
  • IHC - Anti-Human Estrogen Receptor alpha Antibody, clone 6F11  ABD10111
    Formalin fixed, paraffin embedded human breast cancer biopsy stained with Mouse anti Human estrogen receptor alpha antibody followed by HRP polymer detection and DAB substrate develoment following heat mediated antigen retrieval using citrate buffer at pH 6.2 (low power)
  • WB - Anti-Human Estrogen Receptor alpha Antibody, clone 6F11  ABD10111
    Western blot analysis of MCF-7 human breast adenocarcinoma whole cell lysate probed with Mouse anti Human estrogen receptor alpha antibody followed by HRP conjugated Goat anti Mouse IgG, visualized by chemiluminescence
  • IHC-P - Anti-Human Estrogen Receptor alpha Antibody, clone 6F11  ABD10111
    Formalin fixed, paraffin embedded human breast cancer biopsy stained with Mouse anti Human estrogen receptor alpha antibody followed by HRP polymer detection and DAB substrate develoment following heat mediated antigen retrieval using citrate buffer at pH 6.2 (high power)
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F
Primary Accession P03372
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names 6F11
Calculated MW 66216 Da
Additional Information
Immunogen Recombinant human estrogen receptor (alpha form)
Shelf Life 18 months from date of despatch.
Gene ID 2099
Other Names Estrogen receptor, ER, ER-alpha, Estradiol receptor, Nuclear receptor subfamily 3 group A member 1, ESR1, ESR, NR3A1
Target/Specificity Mouse anti-Human estrogen alpha antibody, clone 6F11 recognizes the human estrogen receptor alpha chain (ERα), also known as the estradiol receptor or nuclear receptor subfamily 3 group A member 1. ERα is a ~65kDa steroid hormone receptor containing an N-terminal AF-1).ligand independent transactivation domain, a DNA binding domain and a C-terminal ligand binding domain which overlaps with an AF-2 domain. ERα binds to DNA as a homodimer (Klinge 2001) and can also form heterodimers with Estrogen receptor beta (ERβ) The detection of estrogen (ER) and progesterone (PR) receptors using immunohistochemical staining of formalin fixed, paraffin embedded (FFPE) tissue, has gradually replaced ligand binding assays, to become the most common method for the determination of the ER/PR status of breast tumors (Yazijiet al.2008). Approximately 75% to 80% of breast tumors have estrogen and/or progesterone receptors, and the presence of these receptors helps determine both the patients prognosis and the effectiveness of hormonal therapy (Bhargavaet al.2012).Mouse anti-human estrogen alpha antibody, clone 6F11 has been used successfully for identification of ERα on breast cancer cell lysates using Western blotting and for the immunohistochemical detection of ERα in breast cancer tissues (Ambroiseet al.2011).
Preservative & Stabilisers 0.09% Sodium Azide
Storage Storage at 4℃ or at -20℃.
PrecautionsAnti-Human Estrogen Receptor alpha Antibody, clone 6F11 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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References

1. Bevitt, D.J. et al. (1997) New monoclonal antibodies to oestrogen and progesterone receptors effective for paraffin section immunohistochemistry.
J. Pathol. 183: 228-232. 2. Jongen, V. et al. (2009) Expression of estrogen receptor-alpha and -beta and progesterone receptor-A and -B in a large cohort of patients with endometrioid endometrial cancer.
Gynecol Oncol. 112: 537-42. 3. Ambroise, M. et al. (2011) Immunohistochemical profile of breast cancer patients at a tertiary care hospital in South India.
Asian Pac J Cancer Prev. 12: 625-9.

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Cat# ABD10111
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