|Application ||WB, IHC-P, IHC-F|
|Calculated MW||66216 Da|
|Immunogen||Recombinant human estrogen receptor (alpha form)|
|Shelf Life||18 months from date of despatch.|
|Other Names||Estrogen receptor, ER, ER-alpha, Estradiol receptor, Nuclear receptor subfamily 3 group A member 1, ESR1, ESR, NR3A1|
|Target/Specificity||Mouse anti-Human estrogen alpha antibody, clone 6F11 recognizes the human estrogen receptor alpha chain (ERα), also known as the estradiol receptor or nuclear receptor subfamily 3 group A member 1. ERα is a ~65kDa steroid hormone receptor containing an N-terminal AF-1).ligand independent transactivation domain, a DNA binding domain and a C-terminal ligand binding domain which overlaps with an AF-2 domain. ERα binds to DNA as a homodimer (Klinge 2001) and can also form heterodimers with Estrogen receptor beta (ERβ) The detection of estrogen (ER) and progesterone (PR) receptors using immunohistochemical staining of formalin fixed, paraffin embedded (FFPE) tissue, has gradually replaced ligand binding assays, to become the most common method for the determination of the ER/PR status of breast tumors (Yazijiet al.2008). Approximately 75% to 80% of breast tumors have estrogen and/or progesterone receptors, and the presence of these receptors helps determine both the patients prognosis and the effectiveness of hormonal therapy (Bhargavaet al.2012).Mouse anti-human estrogen alpha antibody, clone 6F11 has been used successfully for identification of ERα on breast cancer cell lysates using Western blotting and for the immunohistochemical detection of ERα in breast cancer tissues (Ambroiseet al.2011).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Storage at 4℃ or at -20℃.|
|Precautions||Anti-Human Estrogen Receptor alpha Antibody, clone 6F11 is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Bevitt, D.J. et al. (1997) New monoclonal antibodies to oestrogen and progesterone receptors effective for paraffin section immunohistochemistry.J. Pathol. 183: 228-232. 2. Jongen, V. et al. (2009) Expression of estrogen receptor-alpha and -beta and progesterone receptor-A and -B in a large cohort of patients with endometrioid endometrial cancer.Gynecol Oncol. 112: 537-42. 3. Ambroise, M. et al. (2011) Immunohistochemical profile of breast cancer patients at a tertiary care hospital in South India.Asian Pac J Cancer Prev. 12: 625-9.
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