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>   home   >   Products   >   Primary Antibodies   >   Immunology   >   Anti-Human CD16 Antibody, clone 2H7    

Anti-Human CD16 Antibody, clone 2H7

Mouse Anti-Human Monoclonal Antibody

     
  • IHC - Anti-Human CD16 Antibody, clone 2H7  ABD10121
    Published customer image:Mouse anti Human CD16 antibody, clone 2H7 used for the demonstration of CD16 expressing cells in epithelia by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.Image caption:Examples of co-localization of CD16+ cells (top row) and gp340 expression (bottom row) in columnar epithelia of the colon/rectum. Serial 5μm sections were stained by IHC for gp340 and CD16, as described in Materials and Methods. The three CD16/gp340 pairs of images represent samples from three different subjects, and each pair is from the same site of the specimen. Gp340 and CD16 are stained brown (some examples marked with arrows), and cell nuclei are blue. Note intraepithelial CD16+ cells and brown granular staining of gp340 throughout the non-mucous columnar cells at the same sites.From: Patyka M, Malamud D, Weissman D, Abrams WR, Kurago Z Periluminal Distribution of HIV-Binding Target Cells and Gp340 in the Oral, Cervical and Sigmoid/Rectal Mucosae: A Mapping Study.PLoS ONE 10: e0132942.
  • IHC-P - Anti-Human CD16 Antibody, clone 2H7  ABD10121
    Published customer image:Mouse anti Human CD16 antibody, clone 2H7 used for the demonstration of CD16 expressing cells in epithelia by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.Image caption:Distribution of CD4+ and CD16+ cells in squamous epithelia of the oral and ectocervical mucosae and in simple columnar epithelia of the rectum/sigmoid and endocervix. Tissue sections were stained by standard IHC for CD4 (Panels A,C,E,G) or CD16 (Panels B,D,F,H), as described in Materials and Methods. Representative images are shown. Receptor-positive cells appear brown and cell nuclei appear blue. Arrows indicate a few examples of positive cells located within the epithelium. Small round CD4bright cells (black arrows) are T cells, while antigen-presenting cells are larger and CD4dim (yellow arrows). Note that CD4+ cells are typically present several cell layers deep within the stratified squamous epithelium and lamina propria of oral (A) and ectocervical mucosae (C), similar to CD16+ APC (B and D, respectively). Small round CD4bright lymphocytes, CD4dim APC and CD16+ APC are also present within the simple columnar epithelia (E-H). Note the horizontal orientation of CD16+ APC at the base of columnar cells in the endocervix (F). Also note that some CD16+ APC in the rectal epithelium are oriented vertically with dendritic processes reaching the luminal surface (insert, H). Bar = 100 μm.From: Patyka M, Malamud D, Weissman D, Abrams WR, Kurago Z Periluminal Distribution of HIV-Binding Target Cells and Gp340 in the Oral, Cervical and Sigmoid/Rectal Mucosae: A Mapping Study.PLoS ONE 10(7): e0132942.
  • IHC-P - Anti-Human CD16 Antibody, clone 2H7  ABD10121
    Immunoperoxidase staining of formalin fixed, paraffin embedded human lung with Mouse anti Human CD16 antibody following heat mediated antigen retrieval using citrate buffer, pH6.0. High power
  • IHC-P - Anti-Human CD16 Antibody, clone 2H7  ABD10121
    Published customer image:Mouse anti Human CD16 antibody, clone 2H7 used for the demonstration of CD16 expressing cells in epithelia by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.Image caption:Interface between the epithelium and the underlying lamina propria is identifiable on the basis of morphology. Sections of colon/rectum (A and B) and of endocervix (C and D) were stained by standard H&E (top panels) or by IHC for CD16. The interface between the epithelium and the lamina propria is indicated with black arrows. CD16+ cells are stained brown. Note many intra-epithelial CD16+ cells (above the basement membrane).From: Patyka M, Malamud D, Weissman D, Abrams WR, Kurago Z Periluminal Distribution of HIV-Binding Target Cells and Gp340 in the Oral, Cervical and Sigmoid/Rectal Mucosae: A Mapping Study.PLoS ONE 10(7): e0132942.
  • IHC-P - Anti-Human CD16 Antibody, clone 2H7  ABD10121
    Immunoperoxidase staining of formalin fixed, paraffin embedded human lung with Mouse anti Human CD16 antibody following heat mediated antigen retrieval using citrate buffer, pH6.0. Low power
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P
Primary Accession O75015
Other Accession P08637
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG2a
Clone Names 2H7
Calculated MW 26216 Da
Additional Information
Immunogen Recombinant protein corresponding to the external domain of the CD16 molecule, common to both the transmembrane form and the GPI-linked form
Shelf Life 18 months from date of despatch.
Gene ID 2215
Other Names Low affinity immunoglobulin gamma Fc region receptor III-B, Fc-gamma RIII-beta, Fc-gamma RIII, Fc-gamma RIIIb, FcRIII, FcRIIIb, FcR-10, IgG Fc receptor III-1, CD16b, FCGR3B, CD16B, FCG3, FCGR3, IGFR3
Target/Specificity Mouse anti-Human CD16 antibody, clone 2H7 recognizes the human Low affinity immunoglobulin gamma Fc region receptor III, also known as CD16. CD 16 exists as both a transmembrane (CD16a) and a GPI-anchored (CD16b) cell surface antigen expressed by granulocytes, NK cells, macrophages and a subset of T-cells. Mouse anti-Human CD16 antibody, clone 2H7 recognizes both forms of the CD16 molecule.
Preservative & Stabilisers 0.09% Sodium Azide
Storage Storage at 4℃ or at -20℃.
PrecautionsAnti-Human CD16 Antibody, clone 2H7 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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References

1. Espinosa, I. et al . (2009) Coordinate expression of colony-stimulating factor-1 and colony-stimulating factor-1-related proteins is associated with poor prognosis in gynecological and nongynecological leiomyosarcoma.
Am J Pathol. 174: 2347-2356. 2. Webster, J.A. et al. (2010) Variations in stromal signatures in breast and colorectal cancer metastases.
J Pathol. 222: 158-65. 3. Wu, J.M. et al. (2011) Endogenous versus tumor-specific host response to breast carcinoma: a study of stromal response in synchronous breast primaries and biopsy site changes.
Clin Cancer Res. 17: 437-46. 4. Beck, A.H. et al. (2009) The macrophage colony-stimulating factor 1 response signature in breast carcinoma.
Clin Cancer Res. 15: 778-87. 5. Tuckerman, E. et al. (2010) Uterine natural killer cells in peri-implantation endometrium from women with repeated implantation failure after IVF.
J Reprod Immunol. 87: 60-6. 6. Ordi, J. et al. (2006) Uterine (CD56+) natural killer cells recruitment: association with decidual reaction rather than embryo implantation.
Am J Reprod Immunol. 55: 369-77. 7. Uccini, S. et al. (2003) In situ study of chemokine and chemokine-receptor expression in Kaposi sarcoma.
Am J Dermatopathol. 25 (5): 377-83. 8. Patyka, M. et al. (2015) Periluminal Distribution of HIV-Binding Target Cells and Gp340 in the Oral, Cervical and Sigmoid/Rectal Mucosae: A Mapping Study.
PLoS One. 10 (7): e0132942.

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