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>   home   >   Products   >   Primary Antibodies   >   Immunology   >   Anti-Human CD163 Antibody, clone EDHu-1    

Anti-Human CD163 Antibody, clone EDHu-1

Mouse Anti-Human Monoclonal Antibody

  • IF - Anti-Human CD163 Antibody, clone EDHu-1  ABD10125
    Immunofluorescence staining of a human tonsil cryosection with Mouse anti Human CD163 antibody, clone EDHu-1 , red in A and Mouse anti Human CD21 antibody, clone LB21 , green in B. C is the merged image with nuclei counterstained blue using DAPI. High power
  • IF - Anti-Human CD163 Antibody, clone EDHu-1  ABD10125
    Published customer image:Mouse anti Human CD163 antibody, clone EDHu-1 used for the detection of CD163 expressing cells in vitro by immunofluorescence.Image caption:Expression of Mf-markers and NF-?B subunits in co-cultures of HSC-3 cells and M1 or R848 Mfs. Vybrant CM-Dil labeled HSC-3 cells and unlabeled M1- or R848 Mfs were incubated with DMSO or 10 ng/ml TNF-a for 30 min where after cells were fixed for immunofluorescence with antibodies for CD68, CD163, NF-?B p50 and p65 subunits. Some samples were pre-incubated with 10 µM BAY 11-7082 prior to TNF-a activation. AlexaFluor488-conjugated secondary antibody was used for visualization. Samples were mounted with DAPI- mountain medium to visualize nuclei . Samples were photographed with a Leica Confocal microscope with 63x oil immersion objective. CD163 and NF-?B p65 immunofluorescence from HSC-3 cell and M1 Mf co-cultures (A). CD68 and NF-?B p50 immunofluorescence from HSC-3 cell and R848 Mf co-cultures (B). Red arrows indicate HSC-3 cells and green arrows Mfs. Scale bars 50 µm.From: Pirilä E, Väyrynen O, Sundquist E, Päkkilä K, Nyberg P, et al. Macrophages Modulate Migration and Invasion of Human Tongue Squamous Cell Carcinoma.PLoS ONE 10(3): e0120895.
  • IF - Anti-Human CD163 Antibody, clone EDHu-1  ABD10125
    Immunofluorescence staining of a human tonsil cryosection with Mouse anti Human CD163 antibody, clone EDHu-1 , red in A and Mouse anti Human CD21 antibody, clone LB21 , green in B. C is the merged image with nuclei counterstained blue using DAPI. Low power
  • FC - Anti-Human CD163 Antibody, clone EDHu-1  ABD10125
    Published customer image:Morphologic and phenotypic characterization of porcine alveolar macrophage cells by flow cytometry using SWC3, SWC1, CD163, CD14, CD16, MHCII and DC-sign staining. This figure is a representative of three independent experiments.From: Seeboth J, Solinhac R, Oswald IP, Guzylack-Piriou L. The fungal T-2 toxin alters the activation of primary macrophages induced by TLR-agonists resulting in a decrease of the inflammatory response in the pig. Vet Res. 2012 Apr 24;43:35..
  • IHC - Anti-Human CD163 Antibody, clone EDHu-1  ABD10125
    Published customer image:Tumor-promoting phenotype of the myeloid clusters. IHC staining demonstrating the expression of CD163, IL-6, IL-10, VEGF-A, MMP-9, SDF-1 and Bcl-xL by the myeloid cells associated with anthracosis. For each protein, representative images showing positive and negative staining areas were selected from the same slide. The quantification was performed by analyzing random images of myeloid cluster areas associated with anthracosis and other areas (10 images for each group) from 10 patients. Two-tailed Student's t-test was used for statistical analysis. Shown are means ± SEM, *** P
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q86VB7
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names EDHu-1
Calculated MW 125451 Da
Additional Information
Other Species Rh,Sh,Pig,Gp
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen Leucocytes harvested from the pleural cavity of patients with idiopathic spontaneous pneumothorax
Shelf Life 18 months from date of despatch.
Gene ID 9332
Other Names Scavenger receptor cysteine-rich type 1 protein M130, Hemoglobin scavenger receptor, CD163, Soluble CD163, sCD163, CD163, M130
Target/Specificity Mouse anti-Human CD163 antibody, clone EDHu-1 recognizes the human CD163 cell surface antigen, a 130-140 kDa glycoprotein expressed by tissue macrophages. CD163 is not expressed by resting peripheral blood leucocytes, but expression may be induced on monocytes by culture in dexamethasone.Clone EDHu-1 is reported to inhibit the binding of haptoglobin/hemoglobin to CD163 (Madsenet al.2004).Truncation mutation analysis demonstrates binding of EDHu-1 occurs via the N-terminal region of CD163 containing the first three scavenger receptor, Cysteine-rich, SRCR domains the third domain being critical as, cleavage of this domain at the major cleavage site ASP-265 abrogates binding to the N-terminal fragment.
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human CD163 Antibody, clone EDHu-1 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Kristiansen, M. et al. (2001) Identification of the haemoglobin scavenger receptor.
Nature. 409 (6817): 198-201. 2. Madsen, M. et al. (2004) Molecular characterization of the haptoglobin.hemoglobin receptor CD163. Ligand binding properties of the scavenger receptor cysteine-rich domain region.
J Biol Chem. 279 (49): 51561-7. 3. Kim, W.K. et al. (2006) CD163 identifies perivascular macrophages in normal and viral encephalitic brains and potential precursors to perivascular macrophages in blood.
Am J Pathol. 168 (3): 822-34. 4. Moreno JA et al. (2010) Peripheral artery disease is associated with a high CD163/TWEAK plasma ratio.
Arterioscler Thromb Vasc Biol. 30 (6): 1253-62. 5. Herrmann-Hoesing, L.M. (2010) Ovine progressive pneumonia virus capsid antigen as found in CD163- and CD172a-positive alveolar macrophages of persistently infected sheep.
Vet Pathol. 47: 518-28. 6. Asleh, R. et al. (2003) Genetically determined heterogeneity in hemoglobin scavenging and susceptibility to diabetic cardiovascular disease.
Circ Res. 92: 1193-200. 7. Fabriek, B.O. et al. (2007) The macrophage CD163 surface glycoprotein is an erythroblast adhesion receptor.
Blood. 109: 5223-9. 8. Jensen, T.O. et al. (2009) Macrophage markers in serum and tumor have prognostic impact in American Joint Committee on Cancer stage I/II melanoma.
J Clin Oncol. 27: 3330-7. 9. Montes de Oca, M. et al. (2005) Skeletal muscle inflammation and nitric oxide in patients with COPD.
Eur Respir J. 26: 390-7. 10. Taus, N.S. et al. (2010) Sheep (Ovis aries) airway epithelial cells support ovine herpesvirus 2 lytic replication in vivo.
Vet Microbiol. 145: 47-53. 11. Vinet-Oliphant, H. et al. (2010) Neurokinin-1 receptor (NK1-R) expression in the brains of SIV-infected rhesus macaques: implications for substance P in NK1-R immune cell trafficking into the CNS.
Am J Pathol. 177: 1286-97. 12. Wang, X. et al. (2006) Monocyte/macrophage and T-cell infiltrates in peritoneum of patients with ovarian cancer or benign pelvic disease.
J Transl Med. 4: 30. 13. Grund, S. et al (2009) The microglial/macrophagic response at the tumour-brain border of invasive meningiomas.
Neuropathol Appl Neurobiol. 35: 82-8. 14. Jorgensen, J.M. et al (2009) Expression level, tissue distribution pattern, and prognostic impact of vascular endothelial growth factors VEGF and VEGF-C and their receptors Flt-1, KDR, and Flt-4 in different subtypes of non-Hodgkin lymphomas.
Leuk Lymphoma. 50: 1647-60. 15. Moreno, J.A. et al. (2009) The CD163-expressing macrophages recognize and internalize TWEAK: potential consequences in atherosclerosis.
Atherosclerosis. 207: 103-10. 16. Tang, Z. et al. (2013) Glucocorticoids Enhance CD163 Expression in Placental Hofbauer Cells.
Endocrinology. 154: 471-82. 17. Boyle, J.J. et al. (2009) Coronary intraplaque hemorrhage evokes a novel atheroprotective macrophage phenotype.
Am J Pathol. 174: 1097-108. 18. Zhang, W. et al. (2013) Myeloid clusters are associated with a pro-metastatic environment and poor prognosis in smoking-related early stage non-small cell lung cancer.
PLoS One. 8: e65121. 19. Seeboth, J. et al. (2012) The fungal T-2 toxin alters the activation of primary macrophages induced by TLR-agonists resulting in a decrease of the inflammatory response in the pig.
Vet Res. 43: 35. 20. Berglin, L. et al.(2014)In situ characterization of intrahepatic non-parenchymal cells in PSC reveals phenotypic patterns associated with disease severity.
PLoS One. 9: e105375. 21. Liu, J. et al. (2014) Evidence for mTOR pathway activation in a spectrum of epilepsy-associated pathologies.
Acta Neuropathol Commun. 2: 71. 22. Baek, J.H. et al. (2014) Extracellular Hb enhances cardiac toxicity in endotoxemic guinea pigs: protective role of haptoglobin.
Toxins (Basel). 6: 1244-59. 23. Micci, L, et al. (2014) CD4 depletion in SIV-infected macaques results in macrophage and microglia infection with rapid turnover of infected cells.
PLoS Pathog. 10: e1004467. 24. Pirilä E et al. (2015) Macrophages modulate migration and invasion of human tongue squamous cell carcinoma.
PLoS One. 10 (3): e0120895. 25. Arranz-Solís D et al. (2016) Systemic and local immune responses in sheep after Neospora caninum experimental infection at early, mid and late gestation.
Vet Res. 47 (1): 2.

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Cat# ABD10125
(40 western blots)
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