|Application ||WB, IHC-P, IHC-F, IF, FC, E|
|Calculated MW||125451 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||Leucocytes harvested from the pleural cavity of patients with idiopathic spontaneous pneumothorax|
|Shelf Life||18 months from date of despatch.|
|Other Names||Scavenger receptor cysteine-rich type 1 protein M130, Hemoglobin scavenger receptor, CD163, Soluble CD163, sCD163, CD163, M130|
|Target/Specificity||Mouse anti-Human CD163 antibody, clone EDHu-1 recognizes the human CD163 cell surface antigen, a 130-140 kDa glycoprotein expressed by tissue macrophages. CD163 is not expressed by resting peripheral blood leucocytes, but expression may be induced on monocytes by culture in dexamethasone.Clone EDHu-1 is reported to inhibit the binding of haptoglobin/hemoglobin to CD163 (Madsenet al.2004).Truncation mutation analysis demonstrates binding of EDHu-1 occurs via the N-terminal region of CD163 containing the first three scavenger receptor, Cysteine-rich, SRCR domains the third domain being critical as, cleavage of this domain at the major cleavage site ASP-265 abrogates binding to the N-terminal fragment.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human CD163 Antibody, clone EDHu-1 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Kristiansen, M. et al. (2001) Identification of the haemoglobin scavenger receptor.Nature. 409 (6817): 198-201. 2. Madsen, M. et al. (2004) Molecular characterization of the haptoglobin.hemoglobin receptor CD163. Ligand binding properties of the scavenger receptor cysteine-rich domain region.J Biol Chem. 279 (49): 51561-7. 3. Kim, W.K. et al. (2006) CD163 identifies perivascular macrophages in normal and viral encephalitic brains and potential precursors to perivascular macrophages in blood.Am J Pathol. 168 (3): 822-34. 4. Moreno JA et al. (2010) Peripheral artery disease is associated with a high CD163/TWEAK plasma ratio.Arterioscler Thromb Vasc Biol. 30 (6): 1253-62. 5. Herrmann-Hoesing, L.M. (2010) Ovine progressive pneumonia virus capsid antigen as found in CD163- and CD172a-positive alveolar macrophages of persistently infected sheep.Vet Pathol. 47: 518-28. 6. Asleh, R. et al. (2003) Genetically determined heterogeneity in hemoglobin scavenging and susceptibility to diabetic cardiovascular disease.Circ Res. 92: 1193-200. 7. Fabriek, B.O. et al. (2007) The macrophage CD163 surface glycoprotein is an erythroblast adhesion receptor.Blood. 109: 5223-9. 8. Jensen, T.O. et al. (2009) Macrophage markers in serum and tumor have prognostic impact in American Joint Committee on Cancer stage I/II melanoma.J Clin Oncol. 27: 3330-7. 9. Montes de Oca, M. et al. (2005) Skeletal muscle inflammation and nitric oxide in patients with COPD.Eur Respir J. 26: 390-7. 10. Taus, N.S. et al. (2010) Sheep (Ovis aries) airway epithelial cells support ovine herpesvirus 2 lytic replication in vivo.Vet Microbiol. 145: 47-53. 11. Vinet-Oliphant, H. et al. (2010) Neurokinin-1 receptor (NK1-R) expression in the brains of SIV-infected rhesus macaques: implications for substance P in NK1-R immune cell trafficking into the CNS.Am J Pathol. 177: 1286-97. 12. Wang, X. et al. (2006) Monocyte/macrophage and T-cell infiltrates in peritoneum of patients with ovarian cancer or benign pelvic disease.J Transl Med. 4: 30. 13. Grund, S. et al (2009) The microglial/macrophagic response at the tumour-brain border of invasive meningiomas.Neuropathol Appl Neurobiol. 35: 82-8. 14. Jorgensen, J.M. et al (2009) Expression level, tissue distribution pattern, and prognostic impact of vascular endothelial growth factors VEGF and VEGF-C and their receptors Flt-1, KDR, and Flt-4 in different subtypes of non-Hodgkin lymphomas.Leuk Lymphoma. 50: 1647-60. 15. Moreno, J.A. et al. (2009) The CD163-expressing macrophages recognize and internalize TWEAK: potential consequences in atherosclerosis.Atherosclerosis. 207: 103-10. 16. Tang, Z. et al. (2013) Glucocorticoids Enhance CD163 Expression in Placental Hofbauer Cells.Endocrinology. 154: 471-82. 17. Boyle, J.J. et al. (2009) Coronary intraplaque hemorrhage evokes a novel atheroprotective macrophage phenotype.Am J Pathol. 174: 1097-108. 18. Zhang, W. et al. (2013) Myeloid clusters are associated with a pro-metastatic environment and poor prognosis in smoking-related early stage non-small cell lung cancer.PLoS One. 8: e65121. 19. Seeboth, J. et al. (2012) The fungal T-2 toxin alters the activation of primary macrophages induced by TLR-agonists resulting in a decrease of the inflammatory response in the pig.Vet Res. 43: 35. 20. Berglin, L. et al.(2014)In situ characterization of intrahepatic non-parenchymal cells in PSC reveals phenotypic patterns associated with disease severity.PLoS One. 9: e105375. 21. Liu, J. et al. (2014) Evidence for mTOR pathway activation in a spectrum of epilepsy-associated pathologies.Acta Neuropathol Commun. 2: 71. 22. Baek, J.H. et al. (2014) Extracellular Hb enhances cardiac toxicity in endotoxemic guinea pigs: protective role of haptoglobin.Toxins (Basel). 6: 1244-59. 23. Micci, L, et al. (2014) CD4 depletion in SIV-infected macaques results in macrophage and microglia infection with rapid turnover of infected cells.PLoS Pathog. 10: e1004467. 24. Pirilä E et al. (2015) Macrophages modulate migration and invasion of human tongue squamous cell carcinoma.PLoS One. 10 (3): e0120895. 25. Arranz-Solís D et al. (2016) Systemic and local immune responses in sheep after Neospora caninum experimental infection at early, mid and late gestation.Vet Res. 47 (1): 2.
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