|Application ||WB, IHC-F, IF, FC, E|
|Calculated MW||67405 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Shelf Life||18 months from date of despatch.|
|Other Names||Basal cell adhesion molecule, Auberger B antigen, B-CAM cell surface glycoprotein, F8/G253 antigen, Lutheran antigen, Lutheran blood group glycoprotein, CD239, BCAM, LU, MSK19|
|Target/Specificity||Mouse anti-Human CD239 antibody, clone BRIC221 recognizes human CD239, also known as Lutheran antigen or basal cell aghesion molecule.CD239 is a 597 amino acid, ~85 kDa single pass type I membrane glycoprotein. Clone BRIC221 recognizes a monomorphic determinant expresses on both the 85 and 78 kDa Lutheran (Lu) glycoforms (El Nemer et al.1998). BRIC 221 recognizes an epitope in the fourth extracellular domain of Lu glycoprotein (Parsonset al.1997). Lutheran glycoprotein is a member of the immunoglobulin superfamily and was designated CD239 (B-CAM) at the 7th leucocyte typing workshop. CD239 is expressed by erythrocytes in the peripheral blood.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human CD239 Antibody, clone BRIC221 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Parsons, S.F. et al. (1997) Use of Domain-deletion mutants to locate Lutheran Blood Groups Antigens to each of the five Immunoglobulin Superfamily Domains of the Lutheran Glycoprotein; Elucidation and the Aua/AubPolymorphisms.Blood 89: 4219-4225. 2. Sakai, E. et al. (2007) Construction of recombinant hemagglutinin derived from the gingipain-encoding gene of Porphyromonas gingivalis, identification of its target protein on erythrocytes, and inhibition of hemagglutination by an interdomain regional peptide.J Bacteriol. 189: 3977-86. 3. Bruce, L.J. et al. (2003) A band 3-based macrocomplex of integral and peripheral proteins in the RBC membrane.Blood. 101: 4180-8. 4. Kjellgren, D. et al. (2004) Laminin isoforms in human extraocular muscles.Invest Ophthalmol Vis Sci. 45: 4233-9. 5. Vainionpää, N. et al. (2006) Laminin-10 and Lutheran blood group glycoproteins in adhesion of human endothelial cells.Am J Physiol Cell Physiol. 290: C764-75. 6. Chen, J. et al. (2009) Expression of laminin isoforms, receptors, and binding proteins unique to nucleus pulposus cells of immature intervertebral disc.Connect Tissue Res. 50: 294-306. 7. Määttä, M. et al. (2005) Differential expression of laminin isoforms in ovarian epithelial carcinomas suggesting different origin and providing tools for differential diagnosis.J Histochem Cytochem. 53: 1293-300. 8. Virtanen, I. et al. (2003) Laminin isoforms in fetal and adult human adrenal cortex.J Clin Endocrinol Metab. 88: 4960-6. 9. Kikkawa, Y. et al. (2011) An antibody to the lutheran glycoprotein (Lu) recognizing the LU4 blood type variant inhibits cell adhesion to laminin α5.PLoS One. 6: e23329. 10. Takkunen, M. et al. (2008) Epithelial-mesenchymal transition downregulates laminin alpha5 chain and upregulates laminin alpha4 chain in oral squamous carcinoma cells.Histochem Cell Biol. 130: 509-25. 11. Hasenson, S. et al. (2005) The immortalized human corneal epithelial cells adhere to laminin-10 by using Lutheran glycoproteins and integrin alpha3beta1.Exp Eye Res. 81: 415-21. 12. Liu, J.X. et al. (2011) Different impact of ALS on laminin isoforms in human extraocular muscles versus limb muscles.Invest Ophthalmol Vis Sci. 52: 4842-52. 13. Vuoristo, S. et al. (2009) Laminin isoforms in human embryonic stem cells: synthesis, receptor usage and growth support.J Cell Mol Med. 13: 2622-33.
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