|Application ||WB, IHC-P, IHC-F, IF, FC, E, IP, Func|
|Calculated MW||129295 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||Purified human beta 1 preparation from HT1080 fibrosarcoma cell extract.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Integrin alpha-2, CD49 antigen-like family member B, Collagen receptor, Platelet membrane glycoprotein Ia, GPIa, VLA-2 subunit alpha, CD49b, ITGA2, CD49B|
|Target/Specificity||Mouse anti-Human CD49b antibody, clone 16B4 recognizes human CD49b, also known as integrin α2, collagen receptor, Platelet membrane glycoprotein Ia or VLA-2 subunit alpha. CD49b is a 1181 amino acid ~160kDa single pass type-1 transmembrane glycoprotein posessing multiple FG-GAP repeats and a single VWFA domain. Mouse anti-human CD49b antibody, clone 16B4 is identified as capable of immunoprecipitating a non-reducible alpha subunit from I125 surface labelled Human cell line extract. It is confirmed as specific to the alpha 2 subunit by relative expression of antigen on various cell lines by FACS, and its recognition of affinity purified alpha 2 beta 1 in dot blots.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human CD49b Antibody, clone 16B4 is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Fitter, S. et al. (1999) Transmembrane 4 superfamily protein CD151 (PETA-3) associates with beta 1 and alpha IIb beta 3 integrins in haemopoietic cell lines and modulates cell-cell adhesion.Biochem J. 338 ( Pt 1): 61-70. 2. Sincock, P.M. et al. (1999) PETA-3/CD151, a member of the transmembrane 4 superfamily, is localised to the plasma membrane and endocytic system of endothelial cells, associates with multiple integrins and modulates cell function.J Cell Sci. 112 ( Pt 6): 833-44. 3. Eaton, C.L. et al. (2010) Evaluation of the frequency of putative prostate cancer stem cells in primary and metastatic prostate cancer.Prostate. 70 (8): 875-82. 4. Gassmann, P. et al. (2009) CXCR4 regulates the early extravasation of metastatic tumor cells in vivo.Neoplasia. 11: 651-61. 5. Gassmann, P. et al.(2010)In vivo tumor cell adhesion in the pulmonary microvasculature is exclusively mediated by tumor cell--endothelial cell interaction.BMC Cancer. 10: 177. 6. Ivaska, J. et al. (2005) PKCepsilon-mediated phosphorylation of vimentin controls integrin recycling and motility.EMBO J. 24: 3834-45. 7. Pellinen, T. et al. (2006) Small GTPase Rab21 regulates cell adhesion and controls endosomal traffic of beta1-integrins.J Cell Biol. 173: 767-80. 8. Upla, P. et al. (2004) Clustering induces a lateral redistribution of alpha 2 beta 1 integrin from membrane rafts to caveolae and subsequent protein kinase C-dependent internalization.Mol Biol Cell. 15: 625-36. 9. Jokinen, J. et al. (2010) Molecular mechanism of alpha2beta1 integrin interaction with human echovirus 1.EMBO J. 29: 196-208. 10. Offterdinger, M. et al. (2003) Heregulin and retinoids synergistically induce branching morphogenesis of breast cancer cells cultivated in 3D collagen gels.J Cell Physiol. 195: 260-75. 11. Connors, W.L. and Heino, J. (2005) A duplexed microsphere-based cellular adhesion assay.Anal Biochem. 337: 246-55. 12. Connors, W.L. et al. (2007) Two synergistic activation mechanisms of alpha2beta1 integrin-mediated collagen binding.J Biol Chem. 282: 14675-83. 13. Vuoriluoto, K. et al. (2008) Syndecan-1 supports integrin alpha2beta1-mediated adhesion to collagen.Exp Cell Res. 314: 3369-81. 14. Holleran, B.J. et al. (2003) Differential recruitment of alpha2beta1 and alpha4beta1 integrins to lipid rafts in Jurkat T lymphocytes exposed to collagen type IV and fibronectin.J Leukoc Biol. 73: 243-52. 15. Rintanen, N. et al. (2012) Calpains promote a2ß1 integrin turnover in nonrecycling integrin pathway.Mol Biol Cell. 23: 448-463. 16. Pietiäinen, V. et al. (2004) Echovirus 1 endocytosis into caveosomes requires lipid rafts, dynamin II, and signaling events.Mol Biol Cell. 15: 4911-25.
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