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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Anti-Human Cytokeratin 19 Antibody, clone BA17    

Anti-Human Cytokeratin 19 Antibody, clone BA17

Mouse Anti-Human Monoclonal Antibody

     
  • E - Anti-Human Cytokeratin 19 Antibody, clone BA17  ABD10152
    Sandwich ELISA analysis of human cytokeratin-19 expression using Mouse anti Human cytokeratin 19 antibody, clone BA19 as a capture reagent and biotinylated Mouse anti Human cytokeratin 19 antibody, clone A53-B/A2 as a detection reagent with purified human cytokeratin 19 (Bio-rad catalog; 34002301) as antigen to create the standard curve. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Normal serum (green), colorectal cancer serum (orange) and breast cancer serum (red) samples at 1:4 dilution are shown
  • IHC-P - Anti-Human Cytokeratin 19 Antibody, clone BA17  ABD10152
    Formalin fixed, paraffin embedded human breast cancer biopsy stained with Mouse anti Human cytokeratin 19 antibody followed by HRP polymer detection and DAB substrate development following heat mediated antigen retrieval using citrate buffer at pH6.2 (low power)
  • IHC-P - Anti-Human Cytokeratin 19 Antibody, clone BA17  ABD10152
    Formalin fixed, paraffin embedded human breast cancer biopsy stained with Mouse anti Human cytokeratin 19 antibody followed by HRP polymer detection and DAB substrate development following heat mediated antigen retrieval using citrate buffer at pH6.2 (high power)
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F
Primary Accession P08727
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names BA17
Calculated MW 44106 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein A
Immunogen Mammary organoids.
Shelf Life 18 months from date of despatch.
Gene ID 3880
Other Names Keratin, type I cytoskeletal 19, Cytokeratin-19, CK-19, Keratin-19, K19, KRT19
Target/Specificity Mouse anti-Human Cytokeratin 19 antibody, clone BA17 recognizes the 40kDa cytokeratin corresponding to cytokeratin 19.Cytokeratin 19 is expressed by many simple and non-keratinizing normal and neoplastic epithelia, including glandular and ductal epithelia.
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human Cytokeratin 19 Antibody, clone BA17 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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References

1. Bartek, J. et al. (1985) A subclass of luminal epithelial cells in the human mammary gland, defied by antibodies to cytokeratins.
J. Cell. Sci. 75: 17-33. 2. Bartek, J. et al. (1985) Patterns of expression of keratin 19 as detected with monoclonal antibodies in human breast tissues and tumours.
Int. J. Cancer. 36: 299-306. 3. Chen, Y.K. et al. (2006) Ductular proliferation in liver tissues with severe chronic hepatitis B: an immunohistochemical study.
World J Gastroenterol. 12: 1443-6.

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Cat# ABD10152
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