|Calculated MW||59216 Da|
|Immunogen||Synthetic peptide derived from the C terminus of the human estrogen receptor beta 2 isomer, CGMKMETLLPEATMEQ.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Estrogen receptor beta, ER-beta, Nuclear receptor subfamily 3 group A member 2, ESR2, ESTRB, NR3A2|
|Target/Specificity||Mouse anti-Human Estrogen Receptor beta 2 antibody, clone 57/3 recognizes the estrogen receptor beta 2 isoform, expressed in cell nuclei of a range of human tissues including the ovary, placenta, testis and vas deferens. No cross-reactivity is seen with estrogen receptor beta 1 isoform. The detection of estrogen (ER) and progesterone (PR) receptors using immunohistochemical staining of formal fixed paraffin embedded (FFPE) tissue, has gradually replaced ligand binding assays (LBA), to become the most common method for the determination of the ER/PR status of breast tumors. Approximately 75% to 80% of breast tumors have estrogen and/or progesterone receptors, and the presence of these receptors helps determine both the patient’s prognosis and the effectiveness of hormonal therapy|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human Estrogen Receptor beta 2 Antibody, clone 57/3 is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Saunders P. et al. (2002) ERbeta1 and the ERbeta2 splice variant (ERbetacx/beta2) are expressed in distinct cell populations in the adult human testis.J. Clin. Endocrinol. Metab. 87: 2706-2715. 2. Critchley T. et al. (2002) Wild-type estrogen receptor (ERbeta1) and the splice variant (ERbetacx/beta2) are both expressed within the human endometrium throughout the normal menstrual cycle.J. Clin. Endocrinol. Metab. 87: 5265-5273. 3. Gaskell T. et al. (2003) Differential expression of two estrogen receptor beta isoforms in the human fetal testis during the second trimester of pregnancy.J. Clin. Endocrinol. Metab. 88: 424-432. 4. Scobie G. et al. (2002) Human oestrogen receptors: differential expression of ER alpha and beta and the identification of ER beta variants.Steroids 67: 985-995. 5. Wong, N. A. C. S. et al. (2005) ERbeta isoform expression in colorectal carcinoma: an in vivoandin vitro study of clinicopathological and molecular correlates.J. Pathol. 207: 53-60. 6. Collins, F. et al. (2009) Expression of oestrogen receptors, ERalpha, ERbeta, and ERbeta variants, in endometrial cancers and evidence that prostaglandin F may play a role in regulating expression of ERalpha.BMC Cancer. 16: 330-342. 7. Carpino, A. et al. (2007) Detection of aromatase and estrogen receptors (ERalpha, ERbeta1, ERbeta2) in human Leydig cell tumor.Eur. J. Endocrinol. 157: 239-244. 8. Miller, W.R. et al. (2006) Oestrogen receptor beta and neoadjuvant therapy with tamoxifen: prediction of response and effects of treatment.Br J Cancer. 94: 1333-8. 9. Rago, V. et al. (2009) Identification of ERbeta1 and ERbeta2 in human seminoma, in embryonal carcinoma and in their adjacent intratubular germ cell neoplasia.Reprod Biol Endocrinol. 7: 56. 10. Skliris, G.P. et al. (2006) Expression of oestrogen receptor-beta in oestrogen receptor-alpha negative human breast tumours.Br J Cancer. 95: 616-26. 11. Critchley, H.O. et al. (2002) Wild-type estrogen receptor (ERbeta1) and the splice variant (ERbetacx/beta2) are both expressed within the human endometrium throughout the normal menstrual cycle.J. Clin. Endocrinol. Metab. 87: 5265-73. 12. Ciucci, A. et al. (2014) Gender effect in experimental models of human medulloblastoma: does the estrogen receptor ß signaling play a role?PLoS One. 9 (7): e101623. 13. Ciucci, A. et al. (2015) Mitochondrial estrogen receptor β2 drives antiapoptotic pathways in advanced serous ovarian cancer.Hum Pathol. pii: S0046-8177(15)00120-3. 14. Zannoni, G.F. et al. (2015) Sexual dimorphism in medulloblastoma features.Histopathology. 2015 Jul 1. [Epub ahead of print]
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