|Application ||IHC-F, IF, FC, IP|
|Purification||Purified IgG prepared by affinity chromatography on Protein G|
|Immunogen||Balb/c macrophage precursor cell hybrids|
|Shelf Life||12 months from date of reconstitution.|
|Target/Specificity||Rat anti-Mouse ER-MP58 antibody, clone ER-MP58 recognizes the murine antigen ER-MP58, which is expressed by all bone marrow-derived M-CSF- and GM-CSF-responsive myeloid blood cell precursors.The expression of ER-MP58 remains at a high level throughout the precursor/monocyte stage and is down-regulated upon maturation into mature macrophages. The ER-MP58 antigen is used to distinguish between early myeloid-committed cells and other haematopoietic progenitors cells in the BM. The antigen has been used as a marker of murine macrophage development in the BM.ER-MP58 is suitable for the identification of myeloid haemopoietic islands in various organs, and for embryonal studies.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Mouse ER-MP58 Antibody, clone ER-MP58 is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Leenen, P.J. et al. (1990) Murine macrophage precursor characterization II.Monoclonal antibodies against macrophage precursor antigens. Eur. J. Immunol. 20: 27–34. 2. Henkel, G.W. et al. (1999) Commitment to the monocytic lineage occurs in the absence of the transcription factor PU.1Blood. 93: 2849–2858. 3. Nikolic, T. et al. (2003) Developmental stages of myeloid dendritic cells in mouse bone marrow.Int. Immunol.15: 515-524. 4. Geutskens, S.B. et al. (2005) Macrophages in the murine pancreas and their involvement in fetal endocrine development in vitro.J Leukoc Biol. 78: 845-52. 5. Sunderkötter, C. et al. (2004) Subpopulations of mouse blood monocytes differ in maturation stage and inflammatory response.J Immunol. 172: 4410-7. 6. Chan, J. et al., (1998) Macrophage lineage cells in inflammation: characterization by colony-stimulating factor-1 (CSF-1) receptor (c-Fms), ER-MP58, and ER-MP20 (Ly-6C) expression.Blood. 1998 Aug 92: 1423-31. 7. Sunderkötter, C. et al. (2004) Subpopulations of mouse blood monocytes differ in maturation stage and inflammatory response.J Immunol. 172: 4410-7. 8. Wynn, A.A. et al. (2001) Role of granulocyte/macrophage colony-stimulating factor in zymocel-induced hepatic granuloma formation.Am J Pathol. 158: 131-45. 9. Rössner, S. et al. (2005) Myeloid dendritic cell precursors generated from bone marrow suppress T cell responses via cell contact and nitric oxide production in vitro.Eur J Immunol. 35: 3533-44. 10. Goossens, P. et al. (2011) Myeloid I?Ba deficiency promotes atherogenesis by enhancing leukocyte recruitment to the plaques.PLoS One. 6: e22327. 11. Iwasaki, Y. et al. (2011) In situ proliferation and differentiation of macrophages in dental pulp.Cell Tissue Res. 346: 99-109. 12. Mossink, M.H. et al. (2003) Unimpaired dendritic cell functions in MVP/LRP knockout mice.Immunology. 110: 58-65. 13. Oliveira, M.A. et al. (2003) Immature macrophages derived from mouse bone marrow produce large amounts of IL-12p40 after LPS stimulation.J Leukoc Biol. 74: 857-67. 14. de Bruijn, M.F. et al. (1996) High-level expression of the ER-MP58 antigen on mouse bone marrow hematopoietic progenitor cells marks commitment to the myeloid lineage.Eur J Immunol. 26: 2850-8. 15. Oomen, S.P. et al. (2002) Somatostatin is a selective chemoattractant for primitive (CD34(+)) hematopoietic progenitor cells.Exp Hematol. 30: 116-25. 16. Welzen-Coppens, J.M. et al. (2012) Abnormalities of dendritic cell precursors in the pancreas of the NOD mouse model of diabetes.Eur J Immunol. 42: 186-94. 17. Hoeksema, M.A. et al. (2014) Targeting macrophage Histone deacetylase 3 stabilizes atherosclerotic lesions.EMBO Mol Med. pii: e201404170.
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