|Application ||IHC-F, IF, FC|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||Adherent F1 (CBAxBL) bone marrow stormal cells.|
|Shelf Life||18 months from date of despatch.|
|Target/Specificity||Rat anti-Mouse ER-HR3:Biotin, clone ER-HR3 recognizes the murine antigen ER-HR3. ER-HR3 is a cell surface antigen which is expressed by Langerhans cells in epithelium, a subset of mature macrophages and dendritic cells that are located predominantly in haematopoietic and lymphoid organs. Initial studies of ER-HR3 demonstrated expression on peripheral blood monocytes but recent studies suggest that expression is actually very low.During foetal development, ER-HR3 positive cells are localised to haemapoietic islands and are often associated with erythroid progenitor cells. The functions of the ER-HR3 antigen have not been established but reports suggest that the antigen may be involved in adult erythropoiesis and in the regulation of the immune response.Clone ER-HR3 does not inhibit T cell proliferation in antigen-specific T-cell proliferation studies.Clone ER-HR3 recognizes two proteins of approximately 69kD and 55kD under non-reducing conditions.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Mouse ER-HR3 Antibody, clone ER-HR3 is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. de Jong, J.P. et al. (1994) A monoclonal antibody (ER-HR3) against murine macrophages. I. Ontogeny, distribution and enzyme histochemical characterization of ER-HR3-positive cells.Cell Tissue Res. 275: 567-76. 2. de Jong, J.P. et al. (1994) A monoclonal antibody (ER-HR3) against murine macrophages.II. Biochemical and functional aspects of the ER-HR3 antigen.Cell Tissue Res. 275:577 – 585. 3. Oliveira, M.A. et al. (2003) Immature macrophages derived from mouse bone marrow produce large amounts of IL-12p40 after LPS stimulation.J Leukoc Biol. 74: 857-67. 4. Throsby, M. et al. (2000) CD11c+ eosinophils in the murine thymus: developmental regulation and recruitment upon MHC class I-restricted thymocyte deletion.J Immunol. 165:1965-75. 5. Grabbe, S. et al. (2002) Beta2 integrins are required for skin homing of primed T cells but not for priming naive T cells.J Clin Invest. 109: 183-92. 6. Sonoda, Y. and Sasaki, K. (2012) Hepatic extramedullary hematopoiesis and macrophages in the adult mouse: histometrical and immunohistochemical studies.Cells Tissues Organs. 196: 555-64. 7. Jacobsen, R.N. et al. (2014) Mobilization with granulocyte colony-stimulating factor blocks medullar erythropoiesis by depleting F4/80+VCAM1+CD169+ER-HR3+Ly6G+ erythroid island macrophages in the mouse.Exp Hematol. pii: S0301-472X(14)00139-8.
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