|Application ||IHC-F, FC|
|Calculated MW||109651 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||Balb/c bone marrow derived macrophages.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Aminopeptidase N, AP-N, mAPN, 188.8.131.52, Alanyl aminopeptidase, Aminopeptidase M, AP-M, Membrane protein p161, Microsomal aminopeptidase, CD13, Anpep, Lap-1, Lap1|
|Target/Specificity||Rat anti-Mouse CD13 antibody, clone ER-BMDM1 recognizes the murine homologue of human CD13, a ~160 kDa cell surface antigen with aminopeptidase N activity.In steady state mice, the CD13 antigen is expressed primarily by dendritic cell and mature macrophage subpopulations. The level of CD13 antigen expression increases with maturation, after the monocytic stage. Bone marrow cells and peripheral blood monocytes are reported to be CD13 negative while thioglycollate-elicited macrophages express high levels of CD13.Dendritic cells, interdigitating dendritic cells, veiled cells and Langerhans cells all express CD13, which is reported to be involved in the development of immature dendritic cells from monocytes.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Mouse CD13 Antibody, clone ER-BMDM1 is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Leenen, P.J. et al. (1992) The monoclonal antibody ER-BMDM1 recognizes a macrophage and dendritic cell differentiation antigen with aminopeptidase activity.Eur J Immunol. 22 (6): 1567-72. 2. Leenen, P.J. et al. (1998) Heterogeneity of mouse spleen dendritic cells: in vivo phagocytic activity, expression of macrophage markers, and subpopulation turnover.J Immunol. 160 (5): 2166-73. 3. Sen, A. et al. (2012) Innate immune response to homologous rotavirus infection in the small intestinal villous epithelium at single-cell resolution.Proc Natl Acad Sci U S A. 109: 20667-72. 1. van der Velden, V.H. et al. (2001) CD13/aminopeptidase N involvement in dendritic cell maturation.Leukemia. 15 (1): 190-1.
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