|Application ||WB, IHC-P, E|
|Clone Names||35248 (57/20)|
|Calculated MW||122670 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G|
|Immunogen||Purified recombinant human platelet-derived growth factor (PDGF) receptor alpha (extracellular domain), derived from the insect cell line Sf21.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Platelet-derived growth factor receptor alpha, PDGF-R-alpha, PDGFR-alpha, 184.108.40.206, Alpha platelet-derived growth factor receptor, Alpha-type platelet-derived growth factor receptor, CD140 antigen-like family member A, CD140a antigen, Platelet-derived growth factor alpha receptor, Platelet-derived growth factor receptor 2, PDGFR-2, CD140a, PDGFRA, PDGFR2, RHEPDGFRA|
|Target/Specificity||Mouse anti-Human CD140a antibody, clone 35248 recognizes the human platelet-derived growth factor receptor alpha, a 1089 amino acid, ~180 kDa single pass type I transmembrane glycoprotein.PDGF is a major serum mitogen that can exist as a homo- or heterodimeric protein consisting of disulphide-linked PDGF-A and PDGF-B chains (Hartet al.1990). The PDGF-AA, PDGF-BB and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Whereas PDGF receptor alpha binds all three isoforms with high affinity, PDGF R beta binds PDGF-BB and AB but not PDGF-AA (Hartet al.1988). Both PDGF receptor alpha and beta are members of the class III subfamily of receptor tyrosine kinases (Westermark 1995).Mouse anti-Human CD140a antibody, clone 35248 has been reported for use in functional studies. The ND50 was determined as 0.2-0.6 µg/ml for blocking 10 ng/ml of PDGF-AA biological activity on a quiescent confluent cell culture of human WS1 cells.|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3)|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human CD140a Antibody, clone 35248 (57/20) is for research use only and not for use in diagnostic or therapeutic procedures.|
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1. De Paula, M.L. et al. (2014) The PTEN inhibitor bisperoxovanadium enhances myelination by amplifying IGF-1 signaling in rat and human oligodendrocyte progenitors.Glia. 62 (1): 64-77. 2. Preisner, A. et al. (2015) Non-steroidal anti-inflammatory drug indometacin enhances endogenous remyelination.Acta Neuropathol. 130 (2): 247-61.
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