|Application ||WB, E|
|Calculated MW||57100 Da|
|Purification||Antisera to calf alkaline phosphatase were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.|
|Immunogen||Highly pure calf intestinal alkaline phosphatase.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Intestinal-type alkaline phosphatase, IAP, Intestinal alkaline phosphatase, 220.127.116.11, ALPI|
|Target/Specificity||Rabbit anti-Calf alkaline phosphatase polyclonal antibody recognizes calf intestinal alkaline phosphatase- (IAP), a membrane-bound hydrolase enzyme highly expressed by intestinal mucosa (Hansenet al.2011).Alkaline phosphatase (AP) is a ubiquitously expressed enzyme which removes phosphate groups from target molecules, including DNA, RNA and alkaloids, under alkaline conditions, and is present at higher concentrations in the placenta (placental AP), intestines (intestinal AP) and liver/bone/kidney (tissue non-specific AP). In vivo studies have identified CIAP as a potential therapeutic agent for the treatment of lipopolysaccharide (LPS)-mediated inflammation and sepsis, due to the ability of IAP to neutralise and thereby detoxify LPS (Peterset al.2013).|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Calf Alkaline Phosphatase Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Braccia, A. et al. (2003) Microvillar membrane microdomains exist at physiological temperature. Role of galectin-4 as lipid raft stabilizer revealed by"superrafts".J Biol Chem. 278 (18): 15679-84. 2. Danielsen, E.M. and Hansen, G.H. (2013) Generation of stable lipid raft microdomains in the enterocyte brush border by selective endocytic removal of non-raft membrane.PLoS One. 8: e76661. 3. Hansen, G.H. et al. (2008) Leptin and the obesity receptor (OB-R) in the small intestine and colon: a colocalization study.J Histochem Cytochem. 56: 677-85. 4. Danielsen, E.M. (2015) Probing endocytosis from the enterocyte brush border using fluorescent lipophilic dyes: lipid sorting at the apical cell surface.Histochem Cell Biol. 143 (5): 545-56. 5. Hansen, G.H. et al. (2007) Intestinal alkaline phosphatase: selective endocytosis from the enterocyte brush border during fat absorption.Am J Physiol Gastrointest Liver Physiol. 293: G1325-32. 6. Hansen, G.H. et al. (2011) Dietary free fatty acids form alkaline phosphatase-enriched microdomains in the intestinal brush border membrane.Mol Membr Biol. 28: 136-44. 7. Hansen, G.H. et al. (2009) Endocytic trafficking from the small intestinal brush border probed with FM dye.Am J Physiol Gastrointest Liver Physiol. 297: G708-15. 8. Hansen, G.H. et al. (2009) Lipopolysaccharide-binding protein: localization in secretory granules of Paneth cells in the mouse small intestine.Histochem Cell Biol. 131: 727-32. 9. Danielsen, E.M. et al.(2013)Staphylococcus aureus enterotoxins A- and B: binding to the enterocyte brush border and uptake by perturbation of the apical endocytic membrane traffic.Histochem Cell Biol. 139: 513-24. 10. Danielsen, E.M. et al. (2012) Apolipoprotein A-1 (apoA-1) deposition in, and release from, the enterocyte brush border: a possible role in transintestinal cholesterol efflux (TICE)?Biochim Biophys Acta. 1818 (3): 530-6.1. Beumer, C. et al. (2003) Calf intestinal alkaline phosphatase, a novel therapeutic drug for lipopolysaccharide (LPS)-mediated diseases, attenuates LPS toxicity in mice and piglets.J Pharmacol Exp Ther. 307 (2): 737-44.
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