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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Anti-Calf Alkaline Phosphatase Antibody    

Anti-Calf Alkaline Phosphatase Antibody

Rabbit Anti-Bovine Polyclonal Antibody

  • WB - Anti-Calf Alkaline Phosphatase Antibody  ABD10275
    Published customer image:Rabbit anti intestinal alkaline phosphatase antibody used for western blottingImage caption:DRM analysis of membrane-associated FM. Total membranes (TMs) and DRMs were prepared from mucosal explants cultured for 1 h in the presence of FM (A) or from microvillar membrane vesicles treated with FM (B), as described in Methods. 5 μl (A) -or 2 μl (B) samples of serial dilutions of the membrane fractions were spotted onto Whatman filters and images captured under uv-light. FM was detected in TMs of both mucosal explants and microvillar membrane vesicles, but only in DRMs of the latter. C: SDS/PAGE of TMs and DRMs prepared as described in Methods from either total mucosal- or microvillar membranes. Samples of 25 μl were applied to each well. 1: Mucosal TMs; 2: Mucosal DRMs; 3: Microvillar TMs; 4: Microvillar DRMs. After electrophoresis and transfer onto a PVDF membrane, the lipid raft marker alkaline phosphatase (AP, 67 kDa) and lactase/phlorizin hydrolase (LPH, 160 kDa), a non-raft marker, were visualized by immunoblotting. Total protein was stained with Coomassie brilliant blue. Molecular mass values (kDa) are indicated by arrows.From: Danielsen EM, Hansen GH Generation of Stable Lipid Raft Microdomains in the Enterocyte Brush Border by Selective Endocytic Removal of Non-Raft Membrane. PLoS ONE 8: e76661.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P19111
Reactivity Bovine
Host Rabbit
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 57100 Da
Additional Information
Other Species Pig
Purification Antisera to calf alkaline phosphatase were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Immunogen Highly pure calf intestinal alkaline phosphatase.
Shelf Life 18 months from date of despatch.
Other Names Intestinal-type alkaline phosphatase, IAP, Intestinal alkaline phosphatase,, ALPI
Target/Specificity Rabbit anti-Calf alkaline phosphatase polyclonal antibody recognizes calf intestinal alkaline phosphatase- (IAP), a membrane-bound hydrolase enzyme highly expressed by intestinal mucosa (Hansenet al.2011).Alkaline phosphatase (AP) is a ubiquitously expressed enzyme which removes phosphate groups from target molecules, including DNA, RNA and alkaloids, under alkaline conditions, and is present at higher concentrations in the placenta (placental AP), intestines (intestinal AP) and liver/bone/kidney (tissue non-specific AP). In vivo studies have identified CIAP as a potential therapeutic agent for the treatment of lipopolysaccharide (LPS)-mediated inflammation and sepsis, due to the ability of IAP to neutralise and thereby detoxify LPS (Peterset al.2013).
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Calf Alkaline Phosphatase Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Braccia, A. et al. (2003) Microvillar membrane microdomains exist at physiological temperature. Role of galectin-4 as lipid raft stabilizer revealed by"superrafts".
J Biol Chem. 278 (18): 15679-84. 2. Danielsen, E.M. and Hansen, G.H. (2013) Generation of stable lipid raft microdomains in the enterocyte brush border by selective endocytic removal of non-raft membrane.
PLoS One. 8: e76661. 3. Hansen, G.H. et al. (2008) Leptin and the obesity receptor (OB-R) in the small intestine and colon: a colocalization study.
J Histochem Cytochem. 56: 677-85. 4. Danielsen, E.M. (2015) Probing endocytosis from the enterocyte brush border using fluorescent lipophilic dyes: lipid sorting at the apical cell surface.
Histochem Cell Biol. 143 (5): 545-56. 5. Hansen, G.H. et al. (2007) Intestinal alkaline phosphatase: selective endocytosis from the enterocyte brush border during fat absorption.
Am J Physiol Gastrointest Liver Physiol. 293: G1325-32. 6. Hansen, G.H. et al. (2011) Dietary free fatty acids form alkaline phosphatase-enriched microdomains in the intestinal brush border membrane.
Mol Membr Biol. 28: 136-44. 7. Hansen, G.H. et al. (2009) Endocytic trafficking from the small intestinal brush border probed with FM dye.
Am J Physiol Gastrointest Liver Physiol. 297: G708-15. 8. Hansen, G.H. et al. (2009) Lipopolysaccharide-binding protein: localization in secretory granules of Paneth cells in the mouse small intestine.
Histochem Cell Biol. 131: 727-32. 9. Danielsen, E.M. et al.(2013)Staphylococcus aureus enterotoxins A- and B: binding to the enterocyte brush border and uptake by perturbation of the apical endocytic membrane traffic.
Histochem Cell Biol. 139: 513-24. 10. Danielsen, E.M. et al. (2012) Apolipoprotein A-1 (apoA-1) deposition in, and release from, the enterocyte brush border: a possible role in transintestinal cholesterol efflux (TICE)?
Biochim Biophys Acta. 1818 (3): 530-6.1. Beumer, C. et al. (2003) Calf intestinal alkaline phosphatase, a novel therapeutic drug for lipopolysaccharide (LPS)-mediated diseases, attenuates LPS toxicity in mice and piglets.
J Pharmacol Exp Ther. 307 (2): 737-44.

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Cat# ABD10275
(40 western blots)
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