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>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   Anti-Human Alkaline Phosphatase Antibody    

Anti-Human Alkaline Phosphatase Antibody

Sheep Anti-Human Polyclonal Antibody

     
  • WB - Anti-Human Alkaline Phosphatase Antibody  ABD10276
    Published customer image:Sheep anti Human alkaline phosphatase antibody used for the detection of alkaline phosphatase in Caco-2/TC7 cells by immunoelectron microscopy.Image caption:PPM supply induces movement of SR-BI/CLA-1 towards raft microdomains. (A) Caco-2/TC7 cells were harvested in the presence of Triton X-100 and the lysate fractionated on a 5–40% sucrose gradient. Eleven fractions were collected for immunoblots of SR-BI/CLA-1, EEA1 (early endosome antigen 1) and flottilin-1 (raft marker). (B) Caco-2/TC7 cells were cultured in the absence (control) or presence of PPM or IPM for 10 min and then harvested in the presence of Triton X-100. Cell lysates were applied to a 5–40% sucrose gradient and eleven fractions collected. Fractions 3 to 8 were analyzed by immunoblotting with antibodies against SR-BI/CLA-1 (left panel) and flottilin-1 (right panel). (C) Immunolocalization of SR-BI/CLA-1 and alkaline phosphatase (PLAP, used as raft marker) in the brush border of Caco-2/TC7 cells supplied with PPM. SR-BI/CLA-1 is labelled with anti-rabbit immunoglobulin-gold complexes (18-nm particles) and PLAP with anti-sheep immunoglobulin-gold complexes (12-nm particles). MV, microvilli; bar, 100 nm.From: Béaslas O, Cueille C, Delers F, Chateau D, Chambaz J, Rousset M, et al. Sensing of Dietary Lipids by Enterocytes: A New Role for SR-BI/CLA-1.PLoS ONE 4: e4278.
  • FC - Anti-Human Alkaline Phosphatase Antibody  ABD10276
    Published customer image:Sheep anti Human alkaline phosphatase antibody used for the evaluation of alkaline phosphatase expression in human muscle derived cells by flowcytometry.Image caption:Characterization and phenotype of human mdcs. a. Cultured human mdcs consisted of small, triangular adherent cells and small, round floating cells. Scale bar ?=?50 µm. b. Proliferation curve of one normal human mdc preparation and one DMD mdc preparation . The average mean population doubling time for these cells is 33.79±1.24 hrs. c, d, e, f. Representative images showing the expression of NG2 (c, green), CD49b (d, green), CD29 (e, green) and CD44 (f, green) by pN1 human mdcs. Nuclei were counterstained with 10 µg/ml DAPI . Scale bar ?=?50 µm. g. Representative FACS images showing the expression of PDGFRß, ALP, CD49b, CD29, CD71, CD90, Stro-1, CD56 and CD34 by human mdcs. Isotype control is shown in red in each image, and positive signal is shown in green. Note that most of these markers, apart from PDGFRβ and CD56, were expressed at similar levels among cell preparationsFrom: Meng J, Adkin CF, Xu S-w, Muntoni F, Morgan JE Contribution of Human Muscle-Derived Cells to Skeletal Muscle Regeneration in Dystrophic Host Mice.PLoS ONE 6(3): e17454.
  • FC - Anti-Human Alkaline Phosphatase Antibody  ABD10276
    Published customer image:Sheep anti Human alkaline phosphatase antibody used for the evaluation of alkaline phosphatase expression in pD2 cells by flowcytometry.Image caption:Characterization and phenotype of human mdcs. a. Cultured human mdcs consisted of small, triangular adherent cells and small, round floating cells. Scale bar ?=?50 µm. b. Proliferation curve of one normal human mdc preparation and one DMD mdc preparation . The average mean population doubling time for these cells is 33.79±1.24 hrs. c, d, e, f. Representative images showing the expression of NG2 (c, green), CD49b (d, green), CD29 (e, green) and CD44 (f, green) by pN1 human mdcs. Nuclei were counterstained with 10 µg/ml DAPI . Scale bar ?=?50 µm. g. Representative FACS images showing the expression of PDGFRß, ALP, CD49b, CD29, CD71, CD90, Stro-1, CD56 and CD34 by human mdcs. Isotype control is shown in red in each image, and positive signal is shown in green. Note that most of these markers, apart from PDGFRβ and CD56, were expressed at similar levels among cell preparationsFrom: Meng J, Adkin CF, Xu S-w, Muntoni F, Morgan JE Contribution of Human Muscle-Derived Cells to Skeletal Muscle Regeneration in Dystrophic Host Mice.PLoS ONE 6(3): e17454.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
E, EM
Primary Accession P05186
Other Accession P05187
Reactivity Human
Host Sheep
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 57305 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography
Immunogen Purified alkaline phosphatatse from human placenta.
Shelf Life 18 months from date of despatch.
Gene ID 249
Other Names Alkaline phosphatase, tissue-nonspecific isozyme, AP-TNAP, TNSALP, 3.1.3.1, Alkaline phosphatase liver/bone/kidney isozyme, ALPL
Target/Specificity Sheep anti-Human Alkaline Phosphatase antibody recognizes alkaline phosphatase, it has been found to be reactive with both placental and bone alkaline phosphatase.
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human Alkaline Phosphatase Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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References

1. Béaslas, O. et al. (2009) Sensing of dietary lipids by enterocytes: a new role for SR-BI/CLA-1.
PLoS One. 1: e4278 2. Meng, J. et al. (2011) Contribution of human muscle-derived cells to skeletal muscle regeneration in dystrophic host mice.
PLoS One. 6: e17454.

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