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Anti-Human G-CSF Antibody, clone BVD13-3A5

Rat Anti-Human Monoclonal Antibody

  • E - Anti-Human G-CSF Antibody, clone BVD13-3A5  ABD10317
    Sandwich ELISA analysis of Human G-CSF using Rat anti human G-CSF as a capture reagent and biotinylated Rat anti Human G-CSF as a detection reagent with recombinant human G-CSF as target antigen. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Human serum (orange) and plasma (green) samples are tested undiluted
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P09919
Reactivity Human
Host Rat
Clonality Monoclonal
Isotype IgG1
Clone Names BVD13-3A5
Calculated MW 22293 Da
Additional Information
Immunogen Recombinant human granulocyte-colony stimulating factor expressed in E. coil.
Shelf Life 18 months from date of despatch.
Gene ID 1440
Other Names Granulocyte colony-stimulating factor, G-CSF, Pluripoietin, Filgrastim, Lenograstim, CSF3, C17orf33, GCSF
Target/Specificity Rat anti-Human G-CSF antibody, clone BVD13-3A5 recognizes human granulocyte colony stimulating factor (G-CSF), also known as Pluripoietin. In addition Filgrastim and Lenograstim are used to describe recombinant human G-CSF preparations. Human G-CSF is a 207 amino acid, ~20kDa O-linked glycoprotein belonging to the IL-6 superfamily. There are potentially three isoforms formed by alternative splicing, Rat anti-Human G-CSF antibody, clone BVD13-3A5 binds the canonical isoform-1, binding to other isoforms has not been assessed (UniProt: P09919).G-CSF is produced by activated macrophages, monocytes and neutrophils. In addition it can be produced by fibroblasts and endothelial cells (Zseboet al.1988). G-CSF stimulates production of cells belonging to the granulocytic lineage in the bone marrow and is an activating agent for mature neutrophilic granulocytes (Scapiniet al.2003) to produce other cytokines including CD257 (BAFF), operating through binding to the G-CSF receptor CD114.Rat anti-Human G-CSF antibody, clone BVD13-3A5 has been successfully evaluated as a capture reagent in a highly sensitive sandwich ELISA assay with biotinylated Rat anti-Human G-CSF, clone BVD11-37G10 as a detection reagent and recombinant human G-CSF as antigen.
Preservative & Stabilisers Preservative Free
Storage Store at -20℃ only.
PrecautionsAnti-Human G-CSF Antibody, clone BVD13-3A5 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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Cat# ABD10317
(40 western blots)
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