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>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   Anti-Human Collagen IV Antibody    

Anti-Human Collagen IV Antibody

Goat Anti-Human Polyclonal Antibody

     
  • IF - Anti-Human Collagen IV Antibody  ABD10345
    Published customer image:Goat anti Human collagen IV antibody used for the demonstration of retinal vasculature in mice by immunofluorescence.Image caption:Hypoxia induced endothelial cell proliferation in control and BRVO retinas. (A and B) Representative micrographs of EdU (green)—CollIV (red) double-labeled retinal flatmounts at 7d after BRVO raised in normoxia (A) or hypoxia from d3 to d7 (B). All mice were daily injected with EdU from d3 to d7 before sacrifice (please not that retinas in Fig 2 were injected daily with EdU and are therefor not comparable in terms of numbers of EdU+cells). (C) Quantification of EdU positive ECs of the superior vein and upstream capillary bed at d7 of control retinas (without BRVO) and BRVO retinas, raised in normoxia and hypoxia. All mice were daily injected with EdU from d3 to d7 before sacrifice. Values in histograms are mean ± SEM of mRNA expression of occluded area from 9–10 retinas per group. Mann-Whitney non parametric test, ** p<0.01, normoxic BRVO versus hypoxic BRVO. Scale bars AB = 200μm.From: Dominguez E, Raoul W, Calippe B, Sahel J-A, Guillonneau X, Paques M, et al. Experimental Branch Retinal Vein Occlusion Induces Upstream Pericyte Loss and Vascular Destabilization.PLoS ONE 10: e0132644.
  • IF - Anti-Human Collagen IV Antibody  ABD10345
    Published customer image:Goat anti Human collagen IV antibody used for the demonstration of retinal vasculature in mice by immunofluorescence.Image caption:NG2-staining and pericyte counts after BRVO. (A-D) Representative micrographs of NG2 -CollIV double labeled retinal flatmounts of control (A and B) and at 7d after BRVO (C and D). DAPI is represented in blue in the insets of panel B and D. (E) Quantification of NG2 positive pericytes of control capillaries and capillaries upstream of the occluded vein at indicated time points. Values in histograms are mean ± SEM of number of NG2+ nuclei/mm2 of vascular area. n = 4–18 per group. Mann-Whitney non parametric test, * p<0.05 compared to control (0), intact retina or opposite part of the BRVO retina. Scale bar = 100μm, 25 μm for insets.From: Dominguez E, Raoul W, Calippe B, Sahel J-A, Guillonneau X, Paques M, et al. Experimental Branch Retinal Vein Occlusion Induces Upstream Pericyte Loss and Vascular Destabilization.PLoS ONE 10(7): e0132644.
  • IF - Anti-Human Collagen IV Antibody  ABD10345
    Published customer image:Goat anti Human collagen IV antibody used for the demonstration of retinal vasculature in mice by immunofluorescence.Image caption:Morphometric analysis of vascular network remodeling after BRVO. (A and B) Representative micrographs of CollIV-labeled retinal flatmounts of a control retina (A) and of the occluded vein after BRVO at 3d (B). Positions of the measurements at 800, 1200 and 1600μm from optic nerve were indicated by arrowhead. (C) Measurements of the retinal vein diameter of control retinas or of non occluded side and in the occluded vein at 1, 3 and 7d and indicated distance from the optic nerve after BRVO. Values in histograms are mean ± SEM of diameter (μm) of control or occluded vein in the retina from 4–11 eyes per group. Mann-Whitney non parametric test, * p<0.05 compared to control (0), non occluded veins of the same eye and intact eyes. (D and E) Representative micrographs of CollIV-labeled retinal flatmounts of the superficial capillary network of a control retina (D) and upstream of the occluded vein at 3d (E). (F and G) Quantification of the surface covered by CollIV-positive capillaries (F) and length (capillary length per surface) at different time points. Values in histograms are mean ± SEM of surface or length of capillaries in the retina from 4–18 eyes per group. Mann-Whitney non parametric test, * p<0.001 compared to control (0), non occluded veins of the same eye and non-occluded eyes. Scale bar = 100μm.From: Dominguez E, Raoul W, Calippe B, Sahel J-A, Guillonneau X, Paques M, et al. Experimental Branch Retinal Vein Occlusion Induces Upstream Pericyte Loss and Vascular Destabilization.PLoS ONE 10(7): e0132644.
  • IF - Anti-Human Collagen IV Antibody  ABD10345
    Published c.ustomer image:Goat anti Human collagen IV antibody used for the demonstration of rat retinal vasculature by immunofluorescence.Image caption:Intravitreal injection of STZ does not modify retinal vasculature. A. Glucose transporter GLUT-2 expression in newborn and adult retinas, as compared to adult pancreas. GLUT-2 expression was similar in P6 and adult retina but 22.6 fold lower than in adult pancreas. B–F. P1 rat pups were injected intravitreously with streptozotocin or with the citrate buffer vehicle . B. Retinal flatmounts of P6 CTL or STZ animals were stained using anti-collagen IV antibody. C Representative automated analysis of vascular density using Metamorph software. Branch points are represented by green points and tubes by solid gray lines (lower panel). D–G. Vascular area, mean tube length, branch point density and total tube length density were determined at P6 in hyperglycemic (black bars) or control (white bars) animals. Values in histograms D–G are mean ± SEM of retinas from 8 animals per group from at least 2 different experiments. Data were analyzed by unpaired t-tests. No significant differences were found in these parameters.From: Kermorvant-Duchemin E, Pinel AC, Lavalette S, Lenne D, Raoul W, Calippe B, et al. Neonatal Hyperglycemia Inhibits Angiogenesis and Induces Inflammation and Neuronal Degeneration in the Retina.PLoS ONE 8: e79545.
  • IF - Anti-Human Collagen IV Antibody  ABD10345
    Published customer image:Goat anti Human collagen IV antibody used for the demonstration of rat retinal vasculature by immunofluorescence.Image caption:Figure 4. Astrocytes and pericytes phenotype in NHIR. P1 rat pups were injected with low doses of streptozotocin or with the citrate buffer vehicle . A–D. Retinal flatmounts and sections of P6 CTL (A–B) and STZ (C–D) were co-stained using anti-collagen IV antibody and anti-GFAP antibody. E–F Retinal flatmounts of P6 CTL (E) and STZ (F) were co-stained using anti-collagen IV antibody and or anti-Ng2 antibody. G–H. STZ animals with hyperglycemia >400 mg/DL were co-stained using anti-collagen IV antibody and anti-Ng2 antibody. Nuclei were counterstained with DAPI in A and C. Scale bar 50 μm in A–G, g and g'; 5 μm in E and F insets and H.From: Kermorvant-Duchemin E, Pinel AC, Lavalette S, Lenne D, Raoul W, Calippe B, et al. Neonatal Hyperglycemia Inhibits Angiogenesis and Induces Inflammation and Neuronal Degeneration in the Retina.PLoS ONE 8: e79545.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-F, IF, E
Primary Accession P29400
Other Accession P08572, Q14031, P53420, Q01955, P02462
Reactivity Human
Host Goat
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 161044 Da
Additional Information
Other Species Rat,M
Purification Antiserum to human collagen IV was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Immunogen Human Collagen Type IV from human placenta
Shelf Life 12 months from date of reconstitution.
Gene ID 1287
Other Names Collagen alpha-5(IV) chain, COL4A5
Target/Specificity Goat anti-Human collagen IV antibody recognizes human collagen type IV. It has been cross adsorbed against collagen types I, II, III, V and VI, resulting in
Preservative & Stabilisers Preservative Free
Storage Store at -20℃ only.
PrecautionsAnti-Human Collagen IV Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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References

1. Kermorvant-Duchemin, E. et al. (2013) Neonatal hyperglycemia inhibits angiogenesis and induces inflammation and neuronal degeneration in the retina.
PLoS One. 8(11): e79545. 2. Dominguez, E. et al. (2015) Experimental Branch Retinal Vein Occlusion Induces Upstream Pericyte Loss and Vascular Destabilization.
PLoS One. 10 (7): e0132644.

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Cat# ABD10345
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