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Mouse Anti-Human Monoclonal Antibody
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Application 
| WB, IHC-P, IHC-F, E |
|---|---|
| Primary Accession | P15941 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone Names | VU-4H5 |
| Calculated MW | 122102 Da |
| Purification | Purified IgG prepared by affinity chromatography on Protein A |
|---|---|
| Immunogen | 60-mer tandem repeat of VTSAPDTRPAPGSTAPPAHG, conjugated to BSA. |
| Shelf Life | 18 months from date of despatch. |
| Gene ID | 4582 |
| Other Names | Mucin-1, MUC-1, Breast carcinoma-associated antigen DF3, Cancer antigen 15-3, CA 15-3, Carcinoma-associated mucin, Episialin, H23AG, Krebs von den Lungen-6, KL-6, PEMT, Peanut-reactive urinary mucin, PUM, Polymorphic epithelial mucin, PEM, Tumor-associated epithelial membrane antigen, EMA, Tumor-associated mucin, CD227, Mucin-1 subunit alpha, MUC1-NT, MUC1-alpha, Mucin-1 subunit beta, MUC1-beta, MUC1-CT, MUC1, PUM |
| Target/Specificity | Mouse anti-Human CD227 antibody, clone VU-4H5 recognizes human Mucin 1, also known as CD227, Cancer antigen 15-3, Episialin or Krebs von den Lungen-6. CD227 is a 1255 amino acid single pass type I transmembrane glycoprotein. Mucins are a family of high molecular weight, heavily glycosylated proteins (glycoconjugates) produced by many epithelial tissues in vertebrates. CD227 is a breast cancer associated mucin encoded by the Muc-1 gene. CD227 is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. This protein is overexpressed abundantly in >90% breast carcinomas and metastases.Mouse anti-Human CD227 antibody, clone VU-4H5 reacts with the APDTR peptide sequence (VTSAPDTRPAPGSTAPPAHG) of human CD227. |
| Preservative & Stabilisers | 0.09% Sodium Azide (NaN3) |
| Storage | Store at +4℃ or at -20 ℃. |
| Precautions | Anti-Human CD227 Antibody, clone VU-4H5 is for research use only and not for use in diagnostic or therapeutic procedures. |
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Provided below are standard protocols that you may find useful for product applications.
References
1. Treon, S.P. et al. (1999) Muc-1 core protein is expressed on multiple myeloma cells and is induced by dexamethasone.Blood. 93: 1287-98. 2. Karsten, U. et al. (2004) Binding patterns of DTR-specific antibodies reveal a glycosylation-conditioned tumor-specific epitope of the epithelial mucin (MUC1).Glycobiology. 14: 681-92. 3. Treon, S.P. et al. (2000) Elevated soluble MUC1 levels and decreased anti-MUC1 antibody levels in patients with multiple myeloma.Blood. 96: 3147-53. 4. Singh, R. et al. (2007) Target-specific cytotoxic activity of recombinant immunotoxin scFv(MUC1)-ETA on breast carcinoma cells and primary breast tumors.Mol Cancer Ther. 6: 562-9. 5. Vlad, A.M. et al. (2002) Complex carbohydrates are not removed during processing of glycoproteins by dendritic cells: processing of tumor antigen MUC1 glycopeptides for presentation to major histocompatibility complex class II-restricted T cells.J Exp Med. 196: 1435-46. 6. ten Berge, R.L. et al. (2001) MUC1 (EMA) is preferentially expressed by ALK positive anaplastic large cell lymphoma, in the normally glycosylated or only partly hypoglycosylated form.J Clin Pathol. 54: 933-9.
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