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Anti-Human CD227 Antibody, clone VU-4H5

Mouse Anti-Human Monoclonal Antibody

     
  • E - Anti-Human CD227 Antibody, clone VU-4H5  ABD10359
    Sandwich ELISA analysis of CD227 expression using Mouse anti Human CD227, clone 6A4 (BioRad Catalogue 22508250)as a capture reagent and biotinylated Mouse anti Human CD227, clone VU-4H5 as a detection reagent with purified breast cancer antigen (BioRad Catalog 22502313) as antigen for the generation of the standard curve. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Plasma and serum samples diluted 1:5 are indicated in green and orange respectively
  • E - Anti-Human CD227 Antibody, clone VU-4H5  ABD10359
    Sandwich ELISA analysis of CD227 expression using Mouse anti Human CD227, clone C595 as a capture reagent and biotinylated Mouse anti Human CD227, clone VU-4H5 as a detection reagent with purified breast cancer antigen as antigen for the generation of the standard curve. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader . Plasma and serum samples diluted 1:5 are indicated in green and orange respectively
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, E
Primary Accession P15941
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names VU-4H5
Calculated MW 122102 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein A
Immunogen 60-mer tandem repeat of VTSAPDTRPAPGSTAPPAHG, conjugated to BSA.
Shelf Life 18 months from date of despatch.
Gene ID 4582
Other Names Mucin-1, MUC-1, Breast carcinoma-associated antigen DF3, Cancer antigen 15-3, CA 15-3, Carcinoma-associated mucin, Episialin, H23AG, Krebs von den Lungen-6, KL-6, PEMT, Peanut-reactive urinary mucin, PUM, Polymorphic epithelial mucin, PEM, Tumor-associated epithelial membrane antigen, EMA, Tumor-associated mucin, CD227, Mucin-1 subunit alpha, MUC1-NT, MUC1-alpha, Mucin-1 subunit beta, MUC1-beta, MUC1-CT, MUC1, PUM
Target/Specificity Mouse anti-Human CD227 antibody, clone VU-4H5 recognizes human Mucin 1, also known as CD227, Cancer antigen 15-3, Episialin or Krebs von den Lungen-6. CD227 is a 1255 amino acid single pass type I transmembrane glycoprotein. Mucins are a family of high molecular weight, heavily glycosylated proteins (glycoconjugates) produced by many epithelial tissues in vertebrates. CD227 is a breast cancer associated mucin encoded by the Muc-1 gene. CD227 is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. This protein is overexpressed abundantly in >90% breast carcinomas and metastases.Mouse anti-Human CD227 antibody, clone VU-4H5 reacts with the APDTR peptide sequence (VTSAPDTRPAPGSTAPPAHG) of human CD227.
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human CD227 Antibody, clone VU-4H5 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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References

1. Treon, S.P. et al. (1999) Muc-1 core protein is expressed on multiple myeloma cells and is induced by dexamethasone.
Blood. 93: 1287-98. 2. Karsten, U. et al. (2004) Binding patterns of DTR-specific antibodies reveal a glycosylation-conditioned tumor-specific epitope of the epithelial mucin (MUC1).
Glycobiology. 14: 681-92. 3. Treon, S.P. et al. (2000) Elevated soluble MUC1 levels and decreased anti-MUC1 antibody levels in patients with multiple myeloma.
Blood. 96: 3147-53. 4. Singh, R. et al. (2007) Target-specific cytotoxic activity of recombinant immunotoxin scFv(MUC1)-ETA on breast carcinoma cells and primary breast tumors.
Mol Cancer Ther. 6: 562-9. 5. Vlad, A.M. et al. (2002) Complex carbohydrates are not removed during processing of glycoproteins by dendritic cells: processing of tumor antigen MUC1 glycopeptides for presentation to major histocompatibility complex class II-restricted T cells.
J Exp Med. 196: 1435-46. 6. ten Berge, R.L. et al. (2001) MUC1 (EMA) is preferentially expressed by ALK positive anaplastic large cell lymphoma, in the normally glycosylated or only partly hypoglycosylated form.
J Clin Pathol. 54: 933-9.

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Cat# ABD10359
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