|Application ||WB, IHC-P, IHC-F, E|
|Calculated MW||122102 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein A|
|Immunogen||60-mer tandem repeat of VTSAPDTRPAPGSTAPPAHG, conjugated to BSA.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Mucin-1, MUC-1, Breast carcinoma-associated antigen DF3, Cancer antigen 15-3, CA 15-3, Carcinoma-associated mucin, Episialin, H23AG, Krebs von den Lungen-6, KL-6, PEMT, Peanut-reactive urinary mucin, PUM, Polymorphic epithelial mucin, PEM, Tumor-associated epithelial membrane antigen, EMA, Tumor-associated mucin, CD227, Mucin-1 subunit alpha, MUC1-NT, MUC1-alpha, Mucin-1 subunit beta, MUC1-beta, MUC1-CT, MUC1, PUM|
|Target/Specificity||Mouse anti-Human CD227 antibody, clone VU-4H5 recognizes human Mucin 1, also known as CD227, Cancer antigen 15-3, Episialin or Krebs von den Lungen-6. CD227 is a 1255 amino acid single pass type I transmembrane glycoprotein. Mucins are a family of high molecular weight, heavily glycosylated proteins (glycoconjugates) produced by many epithelial tissues in vertebrates. CD227 is a breast cancer associated mucin encoded by the Muc-1 gene. CD227 is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. This protein is overexpressed abundantly in >90% breast carcinomas and metastases.Mouse anti-Human CD227 antibody, clone VU-4H5 reacts with the APDTR peptide sequence (VTSAPDTRPAPGSTAPPAHG) of human CD227.|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3)|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human CD227 Antibody, clone VU-4H5 is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Treon, S.P. et al. (1999) Muc-1 core protein is expressed on multiple myeloma cells and is induced by dexamethasone.Blood. 93: 1287-98. 2. Karsten, U. et al. (2004) Binding patterns of DTR-specific antibodies reveal a glycosylation-conditioned tumor-specific epitope of the epithelial mucin (MUC1).Glycobiology. 14: 681-92. 3. Treon, S.P. et al. (2000) Elevated soluble MUC1 levels and decreased anti-MUC1 antibody levels in patients with multiple myeloma.Blood. 96: 3147-53. 4. Singh, R. et al. (2007) Target-specific cytotoxic activity of recombinant immunotoxin scFv(MUC1)-ETA on breast carcinoma cells and primary breast tumors.Mol Cancer Ther. 6: 562-9. 5. Vlad, A.M. et al. (2002) Complex carbohydrates are not removed during processing of glycoproteins by dendritic cells: processing of tumor antigen MUC1 glycopeptides for presentation to major histocompatibility complex class II-restricted T cells.J Exp Med. 196: 1435-46. 6. ten Berge, R.L. et al. (2001) MUC1 (EMA) is preferentially expressed by ALK positive anaplastic large cell lymphoma, in the normally glycosylated or only partly hypoglycosylated form.J Clin Pathol. 54: 933-9.
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