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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Anti-Human CA125 Antibody, clone X75 (3C8/4)    

Anti-Human CA125 Antibody, clone X75 (3C8/4)

Mouse Anti-Human Monoclonal Antibody

     
  • E - Anti-Human CA125 Antibody, clone X75 (3C8/4)  ABD10360
    Sandwich ELISA analysis of human CA-125 expression using Mouse anti Human CA-125 antibody, clone X75 as a capture reagent and biotinylated Mouse anti Human CA-125 antibody, clone X325 as a detection reagent with purified human CA-125 (Bio-rad Catalog: 83752310) as antigen to generate the standard curve. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Plasma (green) sample at 1:2 is shown
  • E - Anti-Human CA125 Antibody, clone X75 (3C8/4)  ABD10360
    Sandwich ELISA analysis of human CA-125 expression using Mouse anti Human CA-125 antibody, clone X325 as a capture reagent and biotinylated Mouse anti Human CA-125 antibody, clone X75 as a detection reagent with purified human CA-125 (Bio-rad Catalog: 83752310) as antigen to generate the standard curve. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader . Plasma sample at 1:2 is shown
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q8WXI7
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names X75 (3C8/4)
Calculated MW 1519175 Da
Additional Information
Purification Ion Exchange chromatography
Immunogen Native CA-125 (Mucin-16).
Shelf Life 18 months from date of despatch.
Gene ID 94025
Other Names Mucin-16, MUC-16, Ovarian cancer-related tumor marker CA125, CA-125, Ovarian carcinoma antigen CA125, MUC16, CA125
Target/Specificity Mouse anti-Human CA125 antibody, clone X75 (3C8/4) recognizes ovarian cancer antigen CA-125, also known as Mucin-16. Clone X75 recognizes epitope specificity group B (ISOBM classification) similar to M-11.CA-125 is a serum marker, overexpressed in epithelial ovarian cancer cells and is routinely used for patient monitoring. CA-125 was originally identified by Bast et al. using a monoclonal antibody developed against the ovarian cancer cell line OVCA433 (Bastet al.1981).Mouse anti-Human CA125 antibody, clone X75 has been sucessfully used as both a capture reagent and a detection reagent for the development of sensitive ELISA assays for the measurement of CA125 in human plasma samples.
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human CA125 Antibody, clone X75 (3C8/4) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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References

1. Yin, B.W. and Lloyd, K.O. (2001) Molecular cloning of the CA125 ovarian cancer antigen: identification as a newmucin, MUC16.
J Biol Chem. 276: 27371-27375.2. Yin BW. et al. (2002) Ovarian cancer antigen CA125 is encoded by the MUC16 mucin gene.
Int J Cancer. 98: 737-740.

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Cat# ABD10360
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