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Anti-Rat Calcitonin Gene-Related Peptide Antibody

Goat Anti-Rat Polyclonal Antibody

     
  • IF - Anti-Rat Calcitonin Gene-Related Peptide Antibody  ABD10364
    Published customer image:Goat anti Rat calcitonin gene related paptide antibody used for the detection of CRGP expressing axons in rat spinal cord by immunofluorescence.Image caption:Axon fiber composition in paravertebral ganglia and related labeling in spinal cord. A. Lower power image of two caudal sympathetic ganglia and connecting nerve bridge showing nerve contains a mixture of at least 3 neurochemically distinct fiber populations: HB9-GFP+ sympathetic preganglionic neurons (SPNs), CGRP+ visceral afferents, and TH+ sympathetic postganglionics. Boxed regions are enlarged in B and C. B. Enlargement showing SPNs (GFP+), CGRP and TH immunolabeled axons in axon bundle between two ganglia. Image represents composite of 73 consecutive confocal images taken at 0.38 µm optical section thickness (27.74 µm total thickness). C. Enlargement of SPNs (GFP+), CGRP and TH immunolabeling in the left sympathetic ganglion. Section in central region of ganglia shows that, while SPNs appear to form basket-like synapses around postganglionics, CGRP+ afferents do not project through this region. Image is a collapsed stack of 11 consecutive confocal images (4.18 µm total). D. CGRP (red) and HB9-GFP labeling (green) in a transverse section of thoracic spinal cord. Note predominant CGRP labeling in the dorsal horn. HB9-GFP labeling of SPNs in the intermediolateral nucleus and motoneurons (MNs) in the ventral horn are identified on the right side. Middle panel shows CGRP labeling extending ventrally including into the intermediolateral nucleus (at arrow) while right panel provides a higher magnification image showing CGRP labeling intermingled with SPNs in the intermediolateral nucleus as well as along its medially oriented SPN projections. Scale bar is 100 µm in A, 20 µm in B and C, and 50 µm in D.From: Zimmerman AL, Sawchuk M, Hochman S Monoaminergic Modulation of Spinal Viscero-Sympathetic Function in the Neonatal Mouse Thoracic Spinal Cord.PLoS ONE 7: e47213.
  • IF - Anti-Rat Calcitonin Gene-Related Peptide Antibody  ABD10364
    Published customer image:Goat anti calcitonin gene-related peptide antibody used for the detection of CGRP expressing cells by immunofluorescence.Image caption:Localization of PKCd and markers of peptidergic and non-peptidergic dorsal root ganglion neurons. A. Representative images of CGRP+, PKCd+, and co-expressing DRG neurons. B. Graphical representation of total neurons counted and degree of overlap (n = number of neurons). C, D. Representative images of IB4+, PKCd+, and IB4+/PKCd?+?neurons and illustration of overlap. E, F. Representative images of TRPV1+, PKCd+, and TRPV+/PKCd?+?neurons and illustration of overlap. Inset demonstrates TRPV1 antibody stain in TRPV1-KO mice. (Scale bar?=?50 µm; Arrowheads indicate example cells that express both markers).From: Valtcheva MV, Davidson S, Zhao C, Leitges M, Gereau RW 4th. Protein kinase Cδ mediates histamine-evoked itch and responses in pruriceptors.Mol Pain. 2015 Jan 6;11:1.
  • IF - Anti-Rat Calcitonin Gene-Related Peptide Antibody  ABD10364
    Published customer image:Goat anti Rat Calcitonin Gene-Related Peptide antibody used for the detection of CGRP expressing cells in rat plantar hindpaw skin by immunofluorescence.Image caption:Exercise training did not alter hindpaw epidermal innervation or thickness in uninjured or SNI animals. (A, B) 30 μm sections of plantar hindpaw skin were stained with ßIII tubulin and CGRP to quantify total and peptidergic IENF density, respectively. Arrowheads indicate IENF. DAPI staining was used to visualize the granular cell layer of the epidermis and measure epidermal thickness. (C, D) Both total (βIII tubulin+) and peptidergic (CGRP+) IENF density were unchanged by 1–4 weeks of exercise training in uninjured mice, n = 4–6. Student's t-Test. (E) 25 days after injury, a significant reduction in total (βIII tubulin+) IENF density was observed in SNI, control mice compared to uninjured controls (p<0.0001, n = 4). Exercise training for either 2 or 12 hr per night for 2 weeks did not improve hindpaw epidermal innervation following SNI. One-way ANOVA. (F) Epidermal thickness was unchanged by 1–4 weeks of wheel running in uninjured mice, n = 4–6. Student's t-Test. (G) Compared to uninjured controls, SNI, control hindpaw skin trended towards epidermal thinning that was unaffected by 2 or 12 hr/night of wheel running for 2 weeks, n = 4. One-way ANOVA. In panels E and G, mean and SEM of 2 week, 2 hr/night uninjured, controls are represented by sold and dashed lines, respectively. Data are presented as mean ± SEM. In all statistical analyses, a Bonferroni test was performed to correct for multiple comparisons.From: Sheahan TD, Copits BA, Golden JP, Gereau RW IV Voluntary Exercise Training: Analysis of Mice in Uninjured, Inflammatory, and Nerve-Injured Pain States.PLoS ONE 10(7): e0133191.
  • IF - Anti-Rat Calcitonin Gene-Related Peptide Antibody  ABD10364
    Published customer image:Goat anti Rat Calcitonin Gene-Related Peptide antibody used for the detection of CGRP expressing axons in rat spinal cord sections by immunofluorescence.Image caption:Effects of DFO on scarring and regeneration. Tissue preservation, lesion size and axon regeneration at 19 dpl. (A) Exemplary mosaic compositions of PBS- (A) and DFO- (B) treated spinal cord stained with anti-GFAP to visualize the GFAP-negative scar area. Lesion area marked with dotted lines, Asterisks indicate a cystic area in both treatment groups. S3 Fig provides pictures A and B with higher intensity to show that the lesion site is filled with tissue. Straight lines outline the region of interest used for quantification of lesion site (2.5 mm). DFO animals had significantly smaller lesion areas (corrected for the total area) than PBS controls (C) and significantly more tissue sparing (D) and. (E) Quantification of the number of CST axons per mm2 of lesion area revealed a significant increase. (F) the CST tracing was equal in both groups. (G) The number of CGRP axons in the scar was significantly increased in DFO-treated rats. (H, I) Representative pictures of CGRP axon profiles in the lesion area in DFO- and PBS-treated animals. Note that the red spots in I originate from aberrantly stained macrophages. Statistics: unpaired T-test * p < 0.05, ** p < 0.01. Scale bar (G, H) = 50 &mu;m.From: Vogelaar CF, König B, Krafft S, Estrada V, Brazda N, Ziegler B, et al. Pharmacological Suppression of CNS Scarring by Deferoxamine Reduces Lesion Volume and Increases Regeneration in an In Vitro Model for Astroglial-Fibrotic Scarring and in Rat Spinal Cord Injury In Vivo. PLoS ONE 10(7): e0134371.
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, IHC-F, IF, E
Primary Accession P01256
Reactivity Rat
Host Goat
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 13948 Da
Additional Information
Other Species M,Gp,Emu
Purification Purified Ig prepared by affinity chromatography on Protein G
Immunogen Synthetic rat Tyr-CGRP (23-37) conjugated to gamma globulin.
Shelf Life 18 months from date of despatch.
Gene ID 24241
Other Names Calcitonin gene-related peptide 1, Alpha-type CGRP, Calcitonin gene-related peptide I, CGRP-I, Calca, Calc
Target/Specificity Goat anti-Rat Calcitonin Gene-Related Peptide antibody recognizes Calcitonin gene-related peptide, also known as CGRP, a neuropeptide that acts as a vasodilator and may play a role in migraine. Goat anti-Rat Calcitonin Gene-Related Peptide antibody reacts with both the whole molecule (amino acids 1-37) and the C-terminal fragment (23-37).
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Rat Calcitonin Gene-Related Peptide Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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References

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J Physiol. 581: 665-78. 7. Gnanamanickam, G.J. and Llewellyn-Smith, I.J. (2011) Innervation of the rat uterus at estrus: a study in full-thickness, immunoperoxidase-stained whole-mount preparations.
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Proc Natl Acad Sci U S A. 106: 13475-80. 11. Iliff, J.J. et al. (2010) Epoxyeicosatrienoic acids are endogenous regulators of vasoactive neuropeptide release from trigeminal ganglion neurons.
J Neurochem. 115: 1530-42. 12. Iliff, J.J. et al. (2009) Epoxyeicosanoids as mediators of neurogenic vasodilation in cerebral vessels.
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J Chem Neuroanat. 41: 1-12. 14. Zou, M. et al. (2012) Brn3a/Pou4f1 regulates dorsal root ganglion sensory neuron specification and axonal projection into the spinal cord.
Dev Biol. 364: 114-27. 14. Chucair-Elliott, A.J. et al. (2015) Degeneration and Regeneration of Corneal Nerves in Response to HSV-1 Infection.
Invest Ophthalmol Vis Sci. pii: IOVS-14-15596. 15. Yu, W.M. et al. (2009) Disruption of laminin in the peripheral nervous system impedes nonmyelinating Schwann cell development and impairs nociceptive sensory function.
Glia. 57: 850-9. 16. Zimmerman, A.L. et al. (2012) Monoaminergic Modulation of Spinal Viscero-Sympathetic Function in the Neonatal Mouse Thoracic Spinal Cord
PLoS One. 7: e47213. 17. Drummond, E.S. et al. (2013) Increased expression of cutaneous α1-adrenoceptors after chronic constriction injury in rats.
J Pain. pii: S1526-5900(13)01351-5. 18. Li, Z. et al. (2014) Activation of MrgC receptor inhibits N-type calcium channels in small-diameter primary sensory neurons in mice
Pain. pii: S0304-3959(14)00228-0. 19. Drummond, P.D. et al. (2014) Upregulation of α1-adrenoceptors on cutaneous nerve fibres after partial sciatic nerve ligation and in complex regional pain syndrome type II.
Pain. 155: 606-16. 20. Marvaldi L et al. (2015) Enhanced axon outgrowth and improved long-distance axon regeneration in sprouty2 deficient mice.
Dev Neurobiol. 75 (3): 217-31. 21. Pitzer, C. et al. (2008) Granulocyte-colony stimulating factor improves outcome in a mouse model of amyotrophic lateral sclerosis.
Brain. 131: 3335-47. 22. Chucair-Elliott, A.J. et al. (2015) Degeneration and Regeneration of Corneal Nerves in Response to HSV-1 Infection.
Invest Ophthalmol Vis Sci. pii: IOVS-14-15596. 23. Weir, K.A. and Lunam, C.A. (2006) Immunohistochemical study of cutaneous nerves in the emu.
Cell Tissue Res. 326: 697-705. 24. Valtcheva, M.V. et al. (2015) Enhanced non-peptidergic intraepidermal fiber density and an expanded subset of chloroquine-responsive trigeminal neurons in a mouse model of dry skin itch.
J Pain. pii: S1526-5900(15)00043-7. 25. Payne, S.C. et al. (2015) Regeneration of sensory but not motor axons following visceral nerve injury.
Exp Neurol. 266C: 127-142. 26. Van Steenwinckel, J. et al. (2015) Stromal cell-derived CCL2 drives neuropathic pain states through myeloid cell infiltration in injured nerve.
Brain Behav Immun. 45: 198-210. 27. Wong, A.W. et al. (2015) Neurite outgrowth in normal and injured primary sensory neurons reveals different regulation by nerve growth factor (NGF) and artemin.
Mol Cell Neurosci. 65: 125-134. 28. Watanabe, M. et al. (2015) Expression and Regulation of Cav3.2 T-Type Calcium Channels during Inflammatory Hyperalgesia in Mouse Dorsal Root Ganglion Neurons.
PLoS One. 10 (5): e0127572. 29. O'Brien, D.E. et al. (2015) ERK2 Alone Drives Inflammatory Pain But Cooperates with ERK1 in Sensory Neuron Survival.
J Neurosci. 35 (25): 9491-507. 31. Sheahan, T.D. et al. (2015) Voluntary Exercise Training: Analysis of Mice in Uninjured, Inflammatory, and Nerve-Injured Pain States.
PLoS One. 10 (7): e0133191. 32. Park, S.I. et al. (2015) Soft, stretchable, fully implantable miniaturized optoelectronic systems for wireless optogenetics.
Nat Biotechnol. Nov 9. [Epub ahead of print] 33. Vogelaar CF et al. (2015) Pharmacological Suppression of CNS Scarring by Deferoxamine Reduces Lesion Volume and Increases Regeneration in an In Vitro Model for Astroglial-Fibrotic Scarring and in Rat Spinal Cord Injury In Vivo.
PLoS One. 10 (7): e0134371. 34. Chetty, R. et al. (2006) Pancreatic endocrine tumour with ductules: further observations of an unusual histological subtype.
Pathology. 38 (1): 5-9.

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