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>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   Anti-Human Collagen I Antibody, clone NFI/20    

Anti-Human Collagen I Antibody, clone NFI/20

Mouse Anti-Human Monoclonal Antibody

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P08123
Other Accession P02452
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names NFI/20
Calculated MW 129314 Da
Additional Information
Purification Ig fraction prepared by ammonium sulphate precipitation and DEAE-cellulose chromatography
Immunogen Human collagen type I from placenta.
Shelf Life 12 months from date of reconstitution.
Gene ID 1278
Other Names Collagen alpha-2(I) chain, Alpha-2 type I collagen, COL1A2
Target/Specificity Mouse anti-Human Collagen I, clone NFI/20 recognizes human Collagen I composed of trimers consisting of 2 Collagen alpha-2(I) chains and a single Collagen alpha-1(I) chain forming collagen fibrils in ligaments, tendon, skin and bone where they become mineralized by calcium hydroxyapatite. Mouse anti-human Collagen I, clone NFI/20 does not cross-react with collagen types II, III and IV. Collagen is located in the extracellular matrix of connective tissues. It is part of the interacting network of proteoglycans and proteins that provides a structural framework for both soft and calcified connective tissues.Mutations in the encoding gene are associated with osteogenesis imperfecta (Penget al.2012), Ehlers Danlos syndrome (Nuytincket al.2000) and idiopathic juvenile osteoporosis (Pococket al.1995). Defective processing of collagen I by lysyl hydroxylase has also been implicated in Ehlers Danlos syndrome (Ihmeet al.1984),
Preservative & Stabilisers Contains mannitol, dextran & salts
Storage Prior to reconstitution store at +4℃. Following reconstitution store at -20℃.
PrecautionsAnti-Human Collagen I Antibody, clone NFI/20 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Zorin, V. et al. (2014) Clinical-instrumental and morphological evaluation of the effect of autologous dermal fibroblasts administration.
J Tissue Eng Regen Med. Dec 19. [Epub ahead of print] 2. Zorin, V.L. et al. (2014) Octacalcium phosphate ceramics combined with gingiva-derived stromal cells for engineered functional bone grafts.
Biomed Mater. 9(5): 055005. 3. Seedhom, B.B. et al.(2007)In-situ engineering of cartilage repair: a pre-clinical in-vivo exploration of a novel system.
Proc Inst Mech Eng H. 221: 475-88.

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Cat# ABD10411
(40 western blots)
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