|Application ||IHC-F, E|
|Calculated MW||129314 Da|
|Purification||Ig fraction prepared by ammonium sulphate precipitation and DEAE-cellulose chromatography|
|Immunogen||Human collagen type I from placenta.|
|Shelf Life||12 months from date of reconstitution.|
|Other Names||Collagen alpha-2(I) chain, Alpha-2 type I collagen, COL1A2|
|Target/Specificity||Mouse anti-Human Collagen I, clone NFI/20 recognizes human Collagen I composed of trimers consisting of 2 Collagen alpha-2(I) chains and a single Collagen alpha-1(I) chain forming collagen fibrils in ligaments, tendon, skin and bone where they become mineralized by calcium hydroxyapatite. Mouse anti-human Collagen I, clone NFI/20 does not cross-react with collagen types II, III and IV. Collagen is located in the extracellular matrix of connective tissues. It is part of the interacting network of proteoglycans and proteins that provides a structural framework for both soft and calcified connective tissues.Mutations in the encoding gene are associated with osteogenesis imperfecta (Penget al.2012), Ehlers Danlos syndrome (Nuytincket al.2000) and idiopathic juvenile osteoporosis (Pococket al.1995). Defective processing of collagen I by lysyl hydroxylase has also been implicated in Ehlers Danlos syndrome (Ihmeet al.1984),|
|Preservative & Stabilisers||Contains mannitol, dextran & salts|
|Storage||Prior to reconstitution store at +4℃. Following reconstitution store at -20℃.|
|Precautions||Anti-Human Collagen I Antibody, clone NFI/20 is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Zorin, V. et al. (2014) Clinical-instrumental and morphological evaluation of the effect of autologous dermal fibroblasts administration.J Tissue Eng Regen Med. Dec 19. [Epub ahead of print] 2. Zorin, V.L. et al. (2014) Octacalcium phosphate ceramics combined with gingiva-derived stromal cells for engineered functional bone grafts.Biomed Mater. 9(5): 055005. 3. Seedhom, B.B. et al.(2007)In-situ engineering of cartilage repair: a pre-clinical in-vivo exploration of a novel system.Proc Inst Mech Eng H. 221: 475-88.
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