|Application ||WB, IF, E|
|Calculated MW||141785 Da|
|Immunogen||Human (cartilage specific) CNBr-cleaved collagen 2.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Collagen alpha-1(II) chain, Alpha-1 type II collagen, Collagen alpha-1(II) chain, Chondrocalcin, COL2A1|
|Target/Specificity||Mouse anti-Collagen II antibody, clone COLL-II recognizes human collagen type II, the main collagen in cartilaginous tissue. Mouse anti-Collagen II antibody, clone COLL-II reacts with both pepsin solubilised and CNBr-cleaved human and bovine collagen type II. No-cross reactivity is seen with types I, III, V or IX.|
|Preservative & Stabilisers||Preservative Free|
|Storage||Store at -20℃ only.|
|Precautions||Anti-Human Collagen II Antibody, clone COLL-II (Ascites) is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Wilkins, B. S. & Jones, D. B. (1995) Immunohistochemical characterization of intact stromal layers in long-term cultures of human bone marrowBr. J. Haematol. 90: 757 - 766 2. Herrero-Mendez A et al. (2015) HR007: a family of biomaterials based on glycosaminoglycans for tissue repair.J Tissue Eng Regen Med. Mar 2. [Epub ahead of print] 3. Glant, T. T. et al. (1985) Appearance and persistence of fibronectin in cartilage. Specific interaction of fibronectin with collagen type IIHistochemistry 82: 149 - 158. 4. Hashemi Beni, B. et al. (2008) Induction of Chondrogenic Differentiation of Human Adipose-Derived Stem Cells with TGF-ß3 in Pellet Culture System Iranian J basic Med Res. 11: 10-171. Miller, E. J. (1972) Structural studies on cartilage collagen employing limited cleavage and solubilization with pepsinBiochemistry 11, p.4903-4909
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