|Application ||WB, IHC-P, IHC-F, IF, E|
|Calculated MW||137953 Da|
|Purification||Purified IgG prepared by affinity chromatography on immobilized rat collagen I|
|Immunogen||Type I Collagen from rat tail tendon.|
|Shelf Life||12 months from date of reconstitution.|
|Other Names||Collagen alpha-1(I) chain, Alpha-1 type I collagen, Col1a1|
|Target/Specificity||Rabbit anti-Rat Collagen I antibody recognizes both native and heat denatured rat collagen type I. Collagen is located in the extracellular matrix of connective tissues. It is part of the interacting network of proteoglycans and proteins that provides a structural framework for both soft and calcified connective tissues. Type I collagen (~95 kDa) is found in bone, cornea, skin and tendon.Mutations in the encoding gene are associated with osteogenesis imperfecta (Sykeset al.1990), Ehlers Danlos syndrome (Burrowset al.1996), and idiopathic osteoporosis (Pernowet al.2006).Rabbit anti-Rat Collagen I antibody has been used successfully for the quantitation of collagen I in rabbit aortic tissue by western blotting (Valero-Muñozet al.2014).|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3)|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Rat Collagen I Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Daniel, C. et al. (2009) Thrombospondin-2 therapy ameliorates experimental glomerulonephritis via inhibition of cell proliferation, inflammation, and TGF-beta activation.Am J Physiol Renal Physiol. 297: F1299-309. 2. Hartner A, et al. (2006) Angiotensin II formation in the kidney and nephrosclerosis in Ren-2 hypertensive rats.Nephrol Dial Transplant. 21:1778-85. 3. Klanke B, et al. (2008) Blood pressure versus direct mineralocorticoid effects on kidney inflammation andfibrosis in DOCA-salt hypertension.Nephrol Dial Transplant. 23: 3456-63. 4. Jones, H.B. et al. (2012) Quantitative Histopathological Assessment of Retardation of Islets of Langerhans Degeneration in Rosiglitazone-dosed Obese ZDF Rats Using Combined Insulin and Collagens (I and III) Immunohistochemistry with Automated Image Analysis and Statistical Modeling.Toxicol Pathol. 41: 425-44. 5. Valero-Muñoz, M. et al. (2014) Carob pod insoluble fiber exerts anti-atherosclerotic effects in rabbits through sirtuin-1 and peroxisome proliferator-activated receptor-γ coactivator-1α.J Nutr. 144: 1378-84. 6. Aktaş, A. et al. (2013) Ankaferd-Induced Early Soft Tissue Wound Healing in an Experimental Rat Model.Turkiye Klinikleri J Med Sci 2013;33(6): 1344-53.
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