|Application ||IHC-P, IHC-F, IF, E|
|Calculated MW||21422 Da|
|Purification||Purified Ig prepared by affinity chromatography on Protein G|
|Immunogen||Corticotrophin Releasing Factor conjugated to Bovine Serum Albumin.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Corticoliberin, Corticotropin-releasing factor, CRF, Corticotropin-releasing hormone, CRH|
|Target/Specificity||Mouse anti-Human Corticotrophin Releasing Factor antibody, clone 4H9 recognizes human corticotrophin releasing factor (CRF), a hormone found mainly in the hypothalamus that regulates the release of corticotrophin from the pituitary gland.The unique lysine residue (Lys-36) is likely to be involved in the antigenic determinant as lysine modification by CA blocks binding completely. Human serum, containing CRF-binding protein, also inhibits the binding of this antibody to CRF.|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3)|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human Corticotrophin Releasing Factor Antibody, clone 4H9 is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Kravchenko, I. V. & Furalev, V. A. (1994) Monoclonal antibodies directed against two different corticotropin-releasing factor determinants.Hybridoma. 13(1): 59-64. 2. De Bellis, A. et al. (2012) Involvement of Hypothalamus Autoimmunity in Patients with Autoimmune Hypopituitarism: Role of Antibodies to Hypothalamic Cells.J Clin Endocrinol Metab. 97: 3684-3690.
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