|Clone Names||C13 (BGN/C13)|
|Purification||Purified IgG prepared by affinity chromatography on Protein A|
|Immunogen||Cortisol conjugated to bovine serum albumin.|
|Shelf Life||18 months from date of despatch.|
|Target/Specificity||Mouse anti-cortisol monoclonal antibody, clone C13 recognizes cortisol, a glucocorticoid hormone synthesized from cholesterol by the adrenal cortex. It is released in response to pituitary adenocorticotrophic hormone (ACTH) which is, in turn, regulated by corticotrophin releasing factor (CRF). High levels of cortisol act to regulate the release of ACTH and CRF via negative feedback inhibition. The release of cortisol stimulates the conversion of proteins to carbohydrates, raises blood sugar levels and promotes glycogen storage in the liver.The following cross reactivities were observed:Cortisol100%Prednisolone30%Cortisone25%Corticosterone10%11-desoxycortisol5%No reactivity was observed with 17-a-OH-progesterone.|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3)|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Cortisol Antibody, clone C13 (BGN/C13) is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Arya, S.K. et al. (2010) Antibody functionalized interdigitated micro-electrode (IDmicroE) based impedimetric cortisol biosensor.Analyst. 135 (8): 1941-6. 2. Arya, S.K. and Bhansali, S. (2012) Anti-Prostate Specific Antigen (Anti-PSA) Modified Interdigitated Microelectrode-Based Impedimetric Biosensor for PSA DetectionBiosensors Journal 1: H110601 3. Arya, S.K. et al. (2010) Dithiobis(succinimidyl propionate) modified gold microarray electrode based electrochemical immunosensor for ultrasensitive detection of cortisol.Biosens Bioelectron. 25: 2296-301. 4. Arya, S.K. et al. (2011) Polyaniline protected gold nanoparticles based mediator and label free electrochemical cortisol biosensor.Biosens Bioelectron. 28 (1): 166-73. 5. Kaushik, A. et al. (2013) Mediator and label free estimation of stress biomarker using electrophoretically deposited [email protected] hybrid nanocomposite.Biosens Bioelectron. 50: 35-41.
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