|Calculated MW||21307 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Immunogen||A 26 amino acid synthetic peptide corresponding to the N-terminal region of human erythropoietin with the sequence APPRLICDSRVLERYLLEAKEAENIT.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Erythropoietin, Epoetin, EPO|
|Target/Specificity||Mouse anti-Human Erythropoietin antibody, clone AE7A5 recognizes human erythropoietin (EPO) and binds to an epitope within the first 26 amino acids of the N-terminus of human urinary and human recombinant EPO.EPO is a 166 amino acid ~30 kDa glycoprotein hormone secreted primarily by the kidney that stimulates bone marrow erythropoiesis in response to hypoxia or anemia. The cDNAs for Epo have been cloned from many species and the mature proteins from the various species are highly conserved, exhibiting greater than 80% sequence identity at the amino acid level.Mouse anti-Human Erythropoietin antibody, clone AE7A5 is the primary antibody sanctioned by the World anti-Doping Agency for the identification and evaluation of natural and recombinant erythropoietin by western blotting in biological samples (WADA doc: TD2014EPO). In addition to recognizing both recombinant and native human erythropoietin, clone AE7A5 cross reacts with zinc-alpha-2-glycoprotein, this reactivity is well separated from that of erythropoietin and does not interfere with the EPO testing assay (Reichel 2008).|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3)|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human Erythropoietin Antibody, clone AE7A5 is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Sytkowski, A.J. and Fisher, J.W. (1985) Isolation and characterization of an anti-peptide monoclonal antibody to human erythropoietin.J Biol Chem. 260: 14727-31. 2. Lasne, F. et al. (2009) Detection of continuous erythropoietin receptor activator in blood and urine in anti-doping control.Haematologica. 94: 888-90. 2. Lasne, F. et al. (2006) No doubt about the validity of the urine test for detection of recombinant human erythropoietin.Blood. 2006 108:1778-9; author reply 1779-80.1. Sue, J. M. & Sytkowski, A. J. (1983) Site-specific antibodies to human erythropoietin directed toward the NH2-terminal region.PNAS 80(12): 3651-3655.
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