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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Anti-Human alpha Fetoprotein Antibody, clone 2I9/2    

Anti-Human alpha Fetoprotein Antibody, clone 2I9/2

Mouse Anti-Human Monoclonal Antibody

     
  • E - Anti-Human alpha Fetoprotein Antibody, clone 2I9/2  ABD10517
    Sandwich ELISA analysis of alpha fetoprotein binding using Mouse anti Human alpha fetoprotein as a capture reagent and biotinylated Mouse anti Human alpha fetoprotein as a detection reagent with human alpha fetoprotein (Bio-Rad Catalog 13752600) as antigen for the generation of a standard curve. Detection is by HRP conjugated Streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Serum (green) and plasma (orange) samples are both displayed at 1:2 dilution.
  • E - Anti-Human alpha Fetoprotein Antibody, clone 2I9/2  ABD10517
    Sandwich ELISA analysis of alpha fetoprotein binding using Mouse anti Human alpha fetoprotein as a capture reagent and biotinylated Mouse anti Human alpha fetoprotein as a detection reagent with human alpha fetoprotein as antigen for the generation of a standard curve. Detection is by HRP conjugated Streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader . Serum and plasma samples are both displayed at 1:2 dilution.
  • E - Anti-Human alpha Fetoprotein Antibody, clone 2I9/2  ABD10517
    Sandwich ELISA analysis of alpha fetoprotein binding using Mouse anti Human alpha fetoprotein as a capture reagent and biotinylated Mouse anti Human alpha fetoprotein as a detection reagent with human alpha fetoprotein as antigen for the generation of a standard curve. Detection is by HRP conjugated Streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader . Serum and plasma samples are both displayed at 1:2 dilution.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
E
Primary Accession P02771
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG
Clone Names 2I9/2
Calculated MW 68678 Da
Additional Information
Purification Ig fraction prepared by chromatography
Immunogen Human fetal cord serum
Shelf Life 18 months from date of despatch.
Gene ID 174
Other Names Alpha-fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin, AFP, HPAFP
Target/Specificity ABD10517 recognises alpha fetoprotein (AFP), a member of the albuminoid superfamily. It is a 68kD plasma protein found in fetuses over four weeks old, produced by the yolk sac and the fetal liver, with the highest concentration reached during the 12th to 16th week of gestation. It binds and transports various ligands such as bilirubin, fatty acids, steroids, heavy metals and various drugs. After birth, plasma levels drop to trace amounts in healthy adults.Elevated AFP levels are indicative of certain cancers, particularly hepatocellular carcinoma, cancer of the testes or ovaries or a malignant teratoma. During pregnancy increased levels of AFP may indicate neural tube defects such as spina bifida, while decreased levels could indicate Down’s syndrome. ABD10517 does not cross-react with human albumin and other human serum proteins.
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human alpha Fetoprotein Antibody, clone 2I9/2 is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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