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Anti-Human Glucagon (N-Terminal) Antibody

Goat Anti-Human Polyclonal Antibody

     
  • IF - Anti-Human Glucagon (N-Terminal) Antibody  ABD10533
    Published customer image:Goat anti Human glucagon antibody used for the demonstration of glucagon expressing cells in murine embryonic tissue sections by immunofluorescence.Image caption:Spatiotemporal expression of Sel1l during development of the mouse pancreas. Pancreatic sections from timed Sel1l+/βgeo embryos were co-immunostained with antibodies against βGal (red) and various pancreatic markers (green) as indicated on the left side of each panel. (B and H) Magnified views of the boxed area in A and G, respectively. (A-D) βGal expression at E12.5. βGal expression begins in a small number of cells within the core pancreatic epithelium at E12.5 (A). At this stage, there is no co-expression of βGal and SOX9 (B); however, βGal is co-expressed with either glucagon (C) or insulin (D). (E-F) βGal expression at E14.5. βGal is differentially expressed in the PDX1+ cells (E). While high βGal signal was detected in the epithelial branches (arrows), no or very low Gal signal was detected in the core "duct-like" epithelium (arrowhead). These core epithelial cells correspond to the subset of PDX+ cells that express SOX9 (F). (G-J) βGal expression at E15.5. βGal expression increases markedly in the core pancreatic epithelium (G and H, asterisks). βGal co-localizes with either glucagon (I, arrows) or insulin (J, arrows). (K-L) βGal expression at postnatal day 1. βGal is expressed in both islet and acinar tissues (K, asterisk and arrow, respectively). βGal co-localizes with PDX1 in the islet tissue (L). Scale bars: (A, G, I and J) 50 μm; (B-F, K-L) 100 μm.From: Li S, Francisco AB, Munroe RJ, Schimenti JC, Long Q. SEL1L deficiency impairs growth and differentiation of pancreatic epithelial cells.BMC Dev Biol. 2010 Feb 19;10:19.
  • IHC - Anti-Human Glucagon (N-Terminal) Antibody  ABD10533
    Published customer image:Goat anti Human glucagon antibody used for the demonstration of glucagon expressing cells in murine embryonic tissue sections by immunofluorescence.Image caption:Inhibited endocrine and exocrine cell differentiation in Sel1lβgeo/βgeo embryos. (A-F) Immunohistological analysis of the developing pancreas of Sel1l+/+ and Sel1lβgeo/βgeo embryos at E11.5 (A-B) and E15.5 (C-F); the genotypes of the pancreatic sections are indicated as "+/+" and "-/-", respectively. The following antibodies were used: Pdx1 (A-D, green), glucagon (A-D, red), insulin (E-F, red) and amylase (E-F, green). (A-B) At E11.5, Glu+ cells were detected in the pancreatic epithelium of wild-type and Sel1lβgeo/βgeo embryos in equal numbers. (C-D) At E15.5, the number of glucagon+ cells was reduced in Sel1lβgeo/βgeo embryos as compared to wild-type control. (E-F) No amylase+ cells were detected and the number of insulin+ cells was significantly reduced in Sel1lβgeo/βgeo embryos. (G-I) Statistical analyses of the numbers of glucagon+ and insulin+ cells in wild-type and Sel1lβgeo/βgeo embryos. Data were from three wild-type and three Sel1lβgeo/βgeo embryos. At E11.5, no significant difference in the number of glucagon+ cells in wild-type and Sel1lβgeo/βgeo embryos was detected (G). The number of glucagon+ and insulin+ cells was markedly lower in Sel1lβgeo/βgeo embryos than in wild-type control embryos (H and I, respectively). Scale bar: 100 μm.From: Li S, Francisco AB, Munroe RJ, Schimenti JC, Long Q. SEL1L deficiency impairs growth and differentiation of pancreatic epithelial cells.BMC Dev Biol. 2010 Feb 19;10:19.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
E
Primary Accession P01275
Reactivity Human
Host Goat
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 20909 Da
Additional Information
Other Species Pig
Purification Purified IgG prepared by affinity chromatography on Protein G
Immunogen Porcine glucagon, amino acids 1-12, conjugated with bovine thyroglobulin.
Shelf Life 18 months from date of despatch.
Gene ID 2641
Other Names Glucagon, Glicentin, Glicentin-related polypeptide, GRPP, Oxyntomodulin, OXM, OXY, Glucagon, Glucagon-like peptide 1, GLP-1, Incretin hormone, Glucagon-like peptide 1(7-37), GLP-1(7-37), Glucagon-like peptide 1(7-36), GLP-1(7-36), Glucagon-like peptide 2, GLP-2, GCG
Target/Specificity Goat anti-Human glucagon antibody recognizes the pancreatic polypeptide hormone glucagon, whose actions are antagonistic to insulin. Glucagon is produced by A cells within the Islets of Langerhans in the pancreas.Tumors arising from glucagon producing cells are termed glucagonomas, and this antibody may be useful in identifying such tumours.
Preservative & Stabilisers 0.09% Sodium Azide
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human Glucagon (N-Terminal) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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References

1. Li, S. et al. (2010) SEL1L deficiency impairs growth and differentiation of pancreatic epithelial cells.
BMC Dev Biol. 10: 19.

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