|Application ||WB, IHC-P, IHC-F, IP|
|Calculated MW||54787 Da|
|Purification||Purified IgG prepared by affinity chromatography|
|Immunogen||A 12 amino acid peptide corresponding to the carboxyl terminus of the insulin regulatable glucose transporter conjugated to keyhole limpet hemocyanin.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Solute carrier family 2, facilitated glucose transporter member 4, Glucose transporter type 4, insulin-responsive, GLUT-4, SLC2A4, GLUT4|
|Target/Specificity||Rabbit anti-Glucose Transporter 4 antibody recognizes human Solute carrier family 2, facilitated glucose transporter member 4, also known as glucose transporter 4 or GLUT4. GLUT4 is a 509 amino acid ~50 kDa multi-pass transmembrane protein located primarily in the perinuclear region and is translocated to the plasma membrane where it is rapidly reinternalized. Rabbit anti-Glucose Transporter 4 antibody does not react with HEP G2 type glucose transporter in human erythrocytes, rat brain or other tissues that do not exhibit sensitivity to insulin.GLUT4 is the insulin-regulated glucose transporter found in adipose tissue and striated muscle, responsible for glucose disposal. The stimulation of glucose uptake by insulin requires translocation of the GLUT4 glucose transporter from intracellular storage sites to the cell surface. Activation of phosphatidylinositol-3-OH kinase (PI3K) is required for this trafficking event, but it is not sufficient to produce GLUT4 translocation. Human insulin resistance involves a defect in GLUT4 traffic and targeting leading to accumulation in a dense membrane compartment from which insulin is unable to recruit GLUT4 to the cell surface.|
|Preservative & Stabilisers||0.09% Sodium Azide|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Glucose Transporter 4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. James, D. E. et al. (1989) Molecular cloning and characterization of an insulin-regulatable glucose transporter.Nature 338: 83-87. 2. Durante, P.E. et al. (2002) Effects of endurance training on activity and expression of AMP-activated protein kinase isoforms in rat muscles.Am J Physiol Endocrinol Metab 283: E178-186. 3. Chung, M.J. et al. (2010) Anti-diabetic effects of lemon balm ( Melissa officinalis) essential oil on glucose- and lipid-regulating enzymes in type 2 diabetic mice.Br J Nutr. 104 (2): 180-8. 4. Ware, B. et al. (2011) Chronic heart failure selectively induces regional heterogeneity of insulin-responsive glucose transporters.Am J Physiol Regul Integr Comp Physiol. 301: R1300-6. 5. Borghouts, L.B. et al. (2000) GLUT-4 expression is not consistently higher in type-1 than in type-2 fibres of rat and human vastus lateralis muscles; an immunohistochemical study.Pflugers Arch. 441:351-8. 6. Revheim, M.E. et al. (2013) Intermittent and continuous imatinib in a human GIST xenograft model carrying KIT exon 17 resistance mutation D816H.Acta Oncol. 52 (4): 776-82. 7. Iwabe, M. et al. (2014) Increased postexercise insulin sensitivity is accompanied by increased AS160 phosphorylation in slow-twitch soleus muscle.Physiol Rep. 2 (12) pii: e12162.
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