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Anti-Green Fluorescent Protein Antibody

     
  • IF - Anti-Green Fluorescent Protein Antibody  ABD10546
    Published customer image:Sheep anti green fluorescent protein antibody used for the detection of GFP tagged protein by immunofluorescence.Image caption:Overexpression of Khc causes severe reduction of acetylated microtubules and apical domain. Khc-GFP overexpression causes severe reduction of the apical membrane domain in developing pupal eyes (45% pd). (A, B) Localization of Crb (apical marker, red) and E-cad (AJ marker, blue) in the sev-Gal4/+ control (A), and Khc-GFP overexpression by sev-Gal4 (B). Khc-GFP was marked by GFP (green, B'). Khc-GFP caused a loss of Crb (red, arrow, B) and mislocalization of E-cad (blue). (C, D) Localization of Acetub (stabilized microtubule marker, red) and Crb (apical marker, blue) in the sev-Gal4/+ control (C), and sev>Khc-GFP (D). Gal4 caused the reduction of Acetub (red, arrow, D) and Crb (blue). Khc-GFP was marked by GFP (green, D').From: League GP, Nam S-C Role of Kinesin Heavy Chain in Crumbs Localization along the Rhabdomere Elongation in Drosophila Photoreceptor. PLoS ONE 6: e21218.
  • IF - Anti-Green Fluorescent Protein Antibody  ABD10546
    Published customer image:Sheep anti green fluorescent protein antibody used for the detection of GFP tagged Hb9 neurons by immunofluorescence.Image caption:Gfra1-TLZ+/Hb9::GFP- motor neurons display structural and synaptic characteristics of gamma motor neurons. (A) Neurolucida tracings of P20 large Gfra1-TLZ-/Hb9::GFP+ and small strongly Gfra1-TLZ+/Hb9::GFP- MNs . (B-D) Quantitative analyses of dendritic arbors. Gfra1-TLZ-/Hb9::GFP+ MN primary dendrites are more numerous, more highly branched (B) and thicker (C), than those of Gfra1+/Hb9::GFP- MNs. Sholl analysis (D) of Gfra1-TLZ-/Hb9::GFP+ (black line) and Gfra1-TLZ+/Hb9::GFP- (gray line) MNs also reveals differences in the distribution of membrane surface at different distances from soma that are characteristic of a- vs. ?-MNs. (E) VGluT1+ contacts are present on P20 Hb9::GFP+ MNs, but absent on Gfra1-TLZ+ /Hb9::GFP- neurons. (F) VAChT+ contacts are present on both Hb9::GFP+ and Gfra1-TLZ+ /Hb9::GFP- MNs (E and F, regions in white boxes are magnified in insets). (G) Quantification of dendritic and somatic VGluT1 and VAChT positive contacts on Hb9::GFP+ (black bars) and Gfra1-TLZ+/Hb9::GFP- (gray bars) MNs (error bars indicate SEMs; triple and double asterisks indicate significance levels of P < 0.001 and P < 0.01 in t-test comparisons, respectively). (H-K) Tibialis anterior muscle in a Hb9::GFP mouse showing Cy5-bungarotoxin (Cy5-Bgtx, blue) labeled intra- and extrafusal neuromuscular junctions and PGP9.5 immunolabeled sensory and motor axons . Hb9::GFP+ motor axons are in green (H). Spindle afferent annulospiral endings (dual asterisks; also shown in the inset in a serial section) and intrafusal muscle fibers are labeled with PGP9.5. Extrafusal NMJs are innervated by PGP9.5+ and Hb9::GFP+ motor axons (K, high magnification of boxed area). Most motor end-plates on intrafusal fibers lacked GFP. Intrafusal Cy5-Bgtx NMJs (white arrowheads) are innervated by PGP9.5-IR axons that are HB9::GFP- (I, high magnification of boxed area). Yellow arrowheads indicate a few intrafusal NMJs innervated by PGP9.5+ and Hb9::GFP+ motor axons (example boxed and shown at higher magnification in J). Scale bars: (E,F) 20 µm; (H) 200 µm (100 µm in inset); (I,J) 25 µm.From: Shneider NA, Brown MN, Smith CA, Pickel J, Alvarez FJ. Gamma motor neurons express distinct genetic markers at birth and require muscle spindle-derived GDNF for postnatal survival. Neural Dev. 2009 Dec 2;4:42.
  • IF - Anti-Green Fluorescent Protein Antibody  ABD10546
    Published customer image:Sheep anti green fluorescent protein antibody used for the detection of GFP tagged protein by immunofluorescence.Image caption:Khc is dispensable for early eye pattern formation. (A) A null mutant, khc8, was utilized to generate mutant clones in developing third-instar larval eye discs. Crb (apical marker, red) and E-cad (AJ maker, blue) showed little defects in third-instar larval eye discs. (B) A magnified view of (A). Mutant clones were marked by the absence of GFP .From: League GP, Nam S-C Role of Kinesin Heavy Chain in Crumbs Localization along the Rhabdomere Elongation in Drosophila Photoreceptor. PLoS ONE 6(6): e21218.
  • IF - Anti-Green Fluorescent Protein Antibody  ABD10546
    Published customer image:Sheep anti green fluorescent protein antibody used for the detection of GFP tagged protein by immunofluorescence.Image caption:Khc is essential for acetylated microtubules and AJ localization. Stabilized microtubules were stained with Acetub , AJs with E-cad , and wild type cells with GFP . (A-A?) Distal regions of khc8 mutant photoreceptors, marked by the absence of GFP , display reduction and shrinkage of microtubules compared to wild-type cells and AJs at the same location. (B-B?) Proximal regions of khc8 mutant photoreceptors display more severe defects of acetylated microtubules and AJs .From: League GP, Nam S-C Role of Kinesin Heavy Chain in Crumbs Localization along the Rhabdomere Elongation in Drosophila Photoreceptor. PLoS ONE 6(6): e21218.
  • IF - Anti-Green Fluorescent Protein Antibody  ABD10546
    Published customer image:Sheep anti green fluorescent protein antibody used for the detection of GFP tagged protein by immunofluorescence.Image caption:Khc is essential for photoreceptor morphogenesis. khc8 mosaic clone in mid-stage (50% pd) pupal Drosophila photoreceptors. (A–E) khc8 drosophila photoreceptors stained for Crb (apical marker, red), and E-cad (AJ marker, blue). (A'–E') khc8 mutant photoreceptors were marked by the absence of GFP . As the cross sections move more proximally (A–E) both the apical Crb domain and the AJs show increasingly severe defects. Crb is misshapen at the distal section (A, B) and almost absent at the proximal section (E) from the same pupal eye. (F) Developing pupal photoreceptors elongate from distal to proximal direction. Distal (A, B) and proximal (D, E) sections are marked by dashed-lines.From: League GP, Nam S-C Role of Kinesin Heavy Chain in Crumbs Localization along the Rhabdomere Elongation in Drosophila Photoreceptor. PLoS ONE 6(6): e21218.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IF, E
Primary Accession P42212
Host Sheep
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 26886 Da
Additional Information
Purification Purified IgG prepared by affinity chromatography on Protein G.
Immunogen Green fluorescent protein from Aequorea victoria.
Shelf Life 18 months from date of despatch.
Other Names Green fluorescent protein, GFP
Target/Specificity Sheep anti-Green Fluorescent Protein antibody recognizes green fluorescent protein (GFP), a ~27 kDa protein derived from the jellyfish Aequorea victoria. GFP fluoresces green (509nm) when excited by blue light (395nm) and is commonly used as a marker of gene expression.
Preservative & Stabilisers 0.09% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Green Fluorescent Protein Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)

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References

1. Collins, R.T. et al. (2010) MAZe: a tool for mosaic analysis of gene function in zebrafish.
Nat Methods. 7: 219-23. 2. Wu, L. et al. (2011) Properties of a distinct subpopulation of GABAergic commissural interneurons thatare part of the locomotor circuitry in the neonatal spinal cord.
J Neurosci. 31: 4821-33. 3. Knipe, L. et al. (2010) A revised model for the secretion of tPA and cytokines from cultured endothelial cells.
Blood. 116: 2183-91. 4. Shneider, N.A. et al. (2009) Gamma motor neurons express distinct genetic markers at birth and require muscle spindle-derived GDNF for postnatal survival.
Neural Dev. 4: 42. 5. Soza-Ried, C. et al. (2008) Maintenance of thymic epithelial phenotype requires extrinsic signals in mouse and zebrafish.
J Immunol. 181: 5272-7. 6. Lopez, K.A. et al. (2011) Convection-enhanced delivery of topotecan into a PDGF-driven model of glioblastoma prolongs survival and ablates both tumor-initiating cells and recruited glial progenitors.
Cancer Res. 71: 3963-71. 7. League, G.P. and Nam, S.C. (2011) Role of kinesin heavy chain in Crumbs localization along the rhabdomere elongation in Drosophila photoreceptor.
PLoS One. 6:e21218. 8. Siembab, V.C. et al. (2010) Target selection of proprioceptive and motor axon synapses on neonatal V1-derived Ia inhibitory interneurons and Renshaw cells.
J Comp Neurol. 518: 4675-701. 9. Srinivasan, S. et al. (2012) The receptor tyrosine phosphatase Lar regulates adhesion between Drosophila male germline stem cells and the niche.
Development. 139: 1381-90. 10. Haberlandt, C. et al. (2011) Gray matter NG2 cells display multiple Ca2+-signaling pathways and highly motile processes.
PLoS One. 6: e17575. 11. Cheung, L.S. et al. (2013) Dynamic model for the coordination of two enhancers of broad by EGFR signaling.
Proc Natl Acad Sci U S A. 110: 17939-44. 12. Li, X. et al. (2013) Temporal patterning of Drosophila medulla neuroblasts controls neural fates.
Nature. 498: 456-62. 13. Behnia, R. et al. (2014) Processing properties of ON and OFF pathways for Drosophila motion detection.
Nature. 512: 427-30. 14. de Nooij, J.C. et al. (2015) The PDZ-domain protein Whirlin facilitates mechanosensory signaling in mammalian proprioceptors.
J Neurosci. 35 (7): 3073-84. 15. Scotti, M. et al. (2015) Hoxa13:Cre mouse strain for conditional gene manipulation in developing limb, hindgut and urogenital system
Genesis. May 13. [Epub ahead of print] 16. Sun, G.J. et al. (2015) Latent tri-lineage potential of adult hippocampal neural stem cells revealed by Nf1 inactivation.
Nat Neurosci. Nov 2. [Epub ahead of print]1. Adams, K.L. et al. (2015) Foxp1-mediated programming of limb-innervating motor neurons from mouse and human embryonic stem cells.
Nat Commun. 6: 6778.

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