|Application ||IF, E|
|Calculated MW||26886 Da|
|Purification||Purified IgG prepared by affinity chromatography on Protein G.|
|Immunogen||Green fluorescent protein from Aequorea victoria.|
|Shelf Life||18 months from date of despatch.|
|Other Names||Green fluorescent protein, GFP|
|Target/Specificity||Sheep anti-Green Fluorescent Protein antibody recognizes green fluorescent protein (GFP), a ~27 kDa protein derived from the jellyfish Aequorea victoria. GFP fluoresces green (509nm) when excited by blue light (395nm) and is commonly used as a marker of gene expression.|
|Preservative & Stabilisers||0.09% Sodium Azide (NaN3)|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Green Fluorescent Protein Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Collins, R.T. et al. (2010) MAZe: a tool for mosaic analysis of gene function in zebrafish. Nat Methods. 7: 219-23. 2. Wu, L. et al. (2011) Properties of a distinct subpopulation of GABAergic commissural interneurons thatare part of the locomotor circuitry in the neonatal spinal cord.J Neurosci. 31: 4821-33. 3. Knipe, L. et al. (2010) A revised model for the secretion of tPA and cytokines from cultured endothelial cells.Blood. 116: 2183-91. 4. Shneider, N.A. et al. (2009) Gamma motor neurons express distinct genetic markers at birth and require muscle spindle-derived GDNF for postnatal survival.Neural Dev. 4: 42. 5. Soza-Ried, C. et al. (2008) Maintenance of thymic epithelial phenotype requires extrinsic signals in mouse and zebrafish.J Immunol. 181: 5272-7. 6. Lopez, K.A. et al. (2011) Convection-enhanced delivery of topotecan into a PDGF-driven model of glioblastoma prolongs survival and ablates both tumor-initiating cells and recruited glial progenitors.Cancer Res. 71: 3963-71. 7. League, G.P. and Nam, S.C. (2011) Role of kinesin heavy chain in Crumbs localization along the rhabdomere elongation in Drosophila photoreceptor.PLoS One. 6:e21218. 8. Siembab, V.C. et al. (2010) Target selection of proprioceptive and motor axon synapses on neonatal V1-derived Ia inhibitory interneurons and Renshaw cells.J Comp Neurol. 518: 4675-701. 9. Srinivasan, S. et al. (2012) The receptor tyrosine phosphatase Lar regulates adhesion between Drosophila male germline stem cells and the niche.Development. 139: 1381-90. 10. Haberlandt, C. et al. (2011) Gray matter NG2 cells display multiple Ca2+-signaling pathways and highly motile processes.PLoS One. 6: e17575. 11. Cheung, L.S. et al. (2013) Dynamic model for the coordination of two enhancers of broad by EGFR signaling.Proc Natl Acad Sci U S A. 110: 17939-44. 12. Li, X. et al. (2013) Temporal patterning of Drosophila medulla neuroblasts controls neural fates.Nature. 498: 456-62. 13. Behnia, R. et al. (2014) Processing properties of ON and OFF pathways for Drosophila motion detection.Nature. 512: 427-30. 14. de Nooij, J.C. et al. (2015) The PDZ-domain protein Whirlin facilitates mechanosensory signaling in mammalian proprioceptors.J Neurosci. 35 (7): 3073-84. 15. Scotti, M. et al. (2015) Hoxa13:Cre mouse strain for conditional gene manipulation in developing limb, hindgut and urogenital systemGenesis. May 13. [Epub ahead of print] 16. Sun, G.J. et al. (2015) Latent tri-lineage potential of adult hippocampal neural stem cells revealed by Nf1 inactivation.Nat Neurosci. Nov 2. [Epub ahead of print]1. Adams, K.L. et al. (2015) Foxp1-mediated programming of limb-innervating motor neurons from mouse and human embryonic stem cells.Nat Commun. 6: 6778.
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